The DOI matches its Crossref record; 17 of the 17 references it cites were found in a registry or an index.
Human immunodeficiency virus protease expressed in Escherichia coli exhibits autoprocessing and specific maturation of the gag precursor.
Open accessAbstract
The mature gag and pol proteins of human immunodeficiency virus (HIV) and all retroviruses derive from large gag and gag-pol polyprotein precursors by posttranslational cleavage. A highly specific, virally encoded protease is required for this essential proteolytic processing. In this study, the HIV protease gene product was expressed in Escherichia coli and shown to autocatalyze its maturation from a larger precursor. In addition, this bacterially produced HIV protease specifically processed an HIV p55 gag polyprotein precursor when coexpressed in E. coli. This system will allow detailed structure-function analysis of the HIV protease and provides a simple assay for the development of potential therapeutic agents directed against this critical viral enzyme.
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Cites
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Cited by
Active human immunodeficiency virus protease is required for viral infectivity
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Three-dimensional structure of aspartyl protease from human immunodeficiency virus HIV-1
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INHIBITORS OF HIV-1 PROTEASE: A Major Success of Structure-Assisted Drug Design
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Structural biology of HIV 1 1Edited by P. E. Wright
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Metadata
- Authors
- Debouck, C., Gorniak, J. G., Strickler, J. E., Meek, T. D., Metcalf, B. W., & Rosenberg, M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1987-12
- Volume
- 84
- Issue
- 24
- Pages
- 8903-8906
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2028124297
- Cited by
- 278
- References
- 17
- FWCI
- 6.32
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Human immunodeficiency virus protease expressed in Escherichia coli exhibits autoprocessing and specific maturation of the gag precursor. https://app.cochat.ai/papers/10.1073/pnas.84.24.8903
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- Debouck, C., Gorniak, J. G., Strickler, J. E., Meek, T. D., Metcalf, B. W., & Rosenberg, M. (1987). Human immunodeficiency virus protease expressed in Escherichia coli exhibits autoprocessing and specific maturation of the gag precursor. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.84.24.8903
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