Cited by 211DNA Repair MechanismsCarcinogens and Genotoxicity AssessmentDNA and Nucleic Acid Chemistry

The specificity of UV-induced mutations at an endogenous locus in mammalian cells.

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Drobetsky, E A, Grosovsky, A J, Glickman, B W·1987·Proceedings of the National Academy of Sciences

Abstract

We have used a rapid in vivo recombinational method to clone and completely sequence 34 UV-induced mutants at the adenine phosphoribosyltransferase (APRT) locus of Chinese hamster ovary cells. Among the mutants recovered, 26 were single base substitutions including 17 G.C----A.T transitions and a single A.T----G.C transition. Three of the 4 possible transversions accounted for the remaining 8 mutations. The G.C----T.A transversion was not recovered. Six tandem double or closely neighboring double-base substitutions, one double mutation consisting of a G.C----T.A transversion and an adjacent frameshift, as well as one single frameshift mutation were also recovered. UV-induced mutation appears to be targeted to dipyrimidine sites with only two exceptions. These include two double mutations where only one of the base substitutions occurred at a dipyrimidine site. The observed specificity of UV-light-induced mutations at the APRT locus is consistent with the argument that G.C----A.T transitions result primarily from the (6-4) pyrimidine pyrimidone lesion. A striking resemblance in the distribution of UV-induced mutants and a collection of 30 spontaneous mutants identified recently in our laboratory was noted. Both share a common strong site of multiple occurrence and a considerable degree of overlap with respect to site specificity. We speculate therefore that DNA context plays a significant role in mutation fixation in mammalian cells.

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Crossref · 10.1073/pnas.84.24.9103

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Authors
Drobetsky, E. A., Grosovsky, A. J., & Glickman, B. W.
Affiliations
York University (CA)
Venue
Proceedings of the National Academy of Sciences
Published
1987-12
Volume
84
Issue
24
Pages
9103-9107
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2020657751
Cited by
211
References
38
Citations by year
2026: 1 · 2025: 1 · 2024: 3 · 2022: 3 · 2021: 19 · 2018: 1 · 2016: 1 · 2015: 2 · 2014: 7 · 2013: 2 · 2012: 2
FWCI
4.27
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
Keywords
UV-induced mutations, site specificity, adenine phosphoribosyltransferase, CHO cells, base substitutions, transversions, frameshift mutation, spontaneous mutation, mutation fixation
MeSH terms
Adenine Phosphoribosyltransferase, Animals, Base Sequence, Cell Line, Cell Survival, Cloning, Molecular, DNA Damage, Cricetinae, Mutation, Pentosyltransferases

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