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Cited by 153Vector-borne infectious diseasesInsect symbiosis and bacterial influencesBartonella species infections research

Genetic characterization and transovarial transmission of a typhus-like rickettsia found in cat fleas.

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Azad, A F, Sacci, J B, Nelson, W M, Dasch, G A, Schmidtmann, E T, Carl, M·1992·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.89.1.43PMC PMC48171
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Abstract

The identification of apparently fastidious microorganisms is often problematic. DNA from a rickettsia-like agent (called the ELB agent) present in cat fleas could be amplified by PCR with conserved primers derived from rickettsial 17-kDa common protein antigen and citrate synthase genes but not spotted fever group 190-kDa antigen gene. Alu I sites in both the 17-kDa and citrate synthase PCR products obtained with the rickettsia-like agent and Rickettsia typhi were different even though both agents reacted with monoclonal antibodies previously thought specific for R. typhi. The DNA sequence of a portion of the 17-kDa PCR product of the rickettsia-like agent differed significantly from all known rickettsial sequences and resembled the 17-kDa sequences of typhus more than spotted fever group rickettsiae. The rare stable transovarial maintenance of this rickettsia in cat fleas has important implications for the disease potential of cat fleas.

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Authors
Azad, A. F., Sacci, J. B., Nelson, W. M., Dasch, G. A., Schmidtmann, E. T., & Carl, M.
Affiliations
University of Maryland, Baltimore (US)
ORCID
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
1992-01
Volume
89
Issue
1
Pages
43-46
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.89.1.43
PMC
PMC48171
OpenAlex
W2078526251
Cited by
153
References
21
Citations by year
2026: 1 · 2025: 4 · 2024: 1 · 2023: 1 · 2022: 4 · 2021: 2 · 2020: 5 · 2019: 2 · 2018: 4 · 2017: 3 · 2016: 5 · 2015: 4 · 2014: 3 · 2013: 4 · 2012: 3
FWCI
3.97
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health
  • National Institute of Allergy and Infectious Diseases (AI 17828)
  • U.S. Army Medical Research and Development Command
Topics
  • Life Sciences › Immunology and Microbiology › Parasitology › Vector-borne infectious diseases
  • Life Sciences › Agricultural and Biological Sciences › Insect Science › Insect symbiosis and bacterial influences
  • Life Sciences › Immunology and Microbiology › Parasitology › Bartonella species infections research
Keywords
Ctenocephalides felis, transovarial transmission, Rickettsia typhi, citrate synthase, spotted fever group rickettsiae, flea-borne pathogens, genetic characterization
MeSH terms
Animals, Antibodies, Bacterial, Base Sequence, Cats, DNA, Bacterial, Siphonaptera, Molecular Sequence Data, Rickettsia, Rickettsia Infections, Restriction Mapping, Polymerase Chain Reaction

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