Autocrine production of extracellular catalase prevents apoptosis of the human CEM T-cell line in serum-free medium.
Open accessAbstract
CCRF-CEM is a human T-cell line originally isolated from a child with acute lymphoblastic leukemia. At cell densities > 2 x 10(5) cells per ml, CEM cells grow in serum-free medium, but at lower cell densities the cultures rapidly undergo apoptosis, or programmed cell death. The viability of low-density CEM cells could be preserved by supplementing the serum-free medium with "conditioned" medium from high-density CEM cultures, but a variety of known growth factors and lymphokines were ineffective. Fractionation of conditioned medium by sequential chromatography on DEAE-cellulose, propyl agarose, chromatofocusing, and hydrophobic-interaction HPLC resulted in the isolation of a 60-kDa protein capable of sustaining CEM growth in the absence of serum. The active protein was identified as human catalase based on its amino acid sequence and composition and was subsequently shown to exhibit catalase activity and to be replaceable by human erythrocyte catalase or bovine liver catalase. Comparison of the level of intracellular catalase activity with the amount released into the culture medium demonstrated that the latter accounted for < 3% of the total catalase activity present in the cell culture. These findings show that, despite its low amount, the catalase released by CEM cells, and perhaps by T cells in general, provides a critical first line of defense against hydrogen peroxide (H2O2) present in the extracellular milieu.
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- Authors
- Sandstrom, P. A., & Buttke, T. M.
- Affiliations
- East Carolina University (US)
- ORCID
- Sandstrom, P A
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1993-05-15
- Volume
- 90
- Issue
- 10
- Pages
- 4708-4712
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC46582
- OpenAlex
- W2092642041
- Cited by
- 186
- References
- 36
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 3 · 2023: 1 · 2022: 1 · 2021: 4 · 2020: 2 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 1 · 2015: 4 · 2014: 4 · 2013: 4 · 2012: 3
- FWCI
- 4.64
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- The Scholarship East Carolina University's Institutional Repository (East Carolina University) (submittedVersion)
- Funders
- East Carolina University
- National Institutes of Health (1s10rr005554-01)
- National Center for Research Resources (1S10RR05554-01)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cancer and biochemical research
- Keywords
- catalase, autocrine growth factor, apoptosis, serum-free medium, conditioned medium, hydrogen peroxide, reactive oxygen species, T cell survival, acute lymphoblastic leukemia, protein extraction
- MeSH terms
- Amino Acid Sequence, Catalase, Cell Division, Extracellular Space, Growth Substances, Humans, Molecular Sequence Data, T-Lymphocytes, Tumor Cells, Cultured, Apoptosis, In Vitro Techniques
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