Oxidative stress in cell culture: an under‐appreciated problem?

Halliwell, Barry

doi:10.1016/s0014-5793(03)00235-7

Cited by 534Redox biology and oxidative stressAntioxidant Activity and Oxidative StressCancer and biochemical research

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Oxidative stress in cell culture: an under‐appreciated problem?

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Halliwell, Barry·2003·FEBS Letters

DOI 10.1016/s0014-5793(03)00235-7

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Abstract

Cell culture studies have given much valuable information about mechanisms of metabolism and signal transduction and of regulation of gene expression, proliferation, senescence, and death. However, cells in culture may behave differently from cells in vivo in many ways. One of these is that cell culture imposes a state of oxidative stress on cells. I argue that cells that survive and grow in culture might use ROS‐dependent signal transduction pathways that rarely or never operate in vivo. A further problem is that cell culture media can catalyse the oxidation of compounds added to them, resulting in apparent cellular effects that are in fact due to oxidation products such as ROS. Such artefacts may have affected many studies on the effects of ascorbate, thiols, flavonoids and other polyphenolic compounds on cells in culture.

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Cell culture studies are usually carried out under abnormal conditions known as "culture shock," where cells are exposed to high oxygen tension and free metal ions in the medium.
“It is noteworthy that cell culture studies are usually carried out under abnormal conditions known as “culture shock,” where cells are exposed to high oxygen tension and to free metal ions in the medium [27, 55].”
From The Effect of Lycopene Preexposure on UV‐B‐Irradiated Human Keratinocytes (2016) · cites this paper as [55]

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Authors: Halliwell, B.
Affiliations: National University of Singapore (SG)
Corresponding author: Halliwell, Barry
ORCID: Halliwell, Barry
Venue: FEBS Letters
Publisher: Wiley
Published: 2003-03-20
Volume: 540
Issue: 1-3
Pages: 3-6
ISSN: 0014-5793 (ISSN-L)
1873-3468
DOI: 10.1016/s0014-5793(03)00235-7
OpenAlex: W2016860528
Cited by: 534
References: 71
Citations by year: 2026: 12 · 2025: 18 · 2024: 11 · 2023: 15 · 2022: 9 · 2021: 24 · 2020: 20 · 2019: 30 · 2018: 29 · 2017: 27 · 2016: 25 · 2015: 31 · 2014: 21 · 2013: 26 · 2012: 25
FWCI: 9.88
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations: FEBS Letters (publishedVersion)
PubMed (publishedVersion)
National University of Singapore (submittedVersion)
Funders: National University of Singapore
Medical Research Council
National Medical Research Council
Biomedical Research Council
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
Health Sciences › Medicine › Biochemistry (medical) › Antioxidant Activity and Oxidative Stress
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cancer and biochemical research
Keywords: oxidative stress, cell culture, reactive oxygen species, vitamin C, thiols, flavonoids, polyphenols, in vitro-in vivo discrepancy
MeSH terms: Animals, Cells, Cultured, Culture Media, Reactive Oxygen Species, Oxidative Stress

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Oxidative stress in cell culture: an under‐appreciated problem?

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