Direction of microtubule movement is an intrinsic property of the motor domains of kinesin heavy chain and Drosophila ncd protein.

Stewart, R J; Thaler, J P; Goldstein, L S

doi:10.1073/pnas.90.11.5209

Cited by 145Microtubule and mitosis dynamicsGenetic and Kidney Cyst DiseasesProtein Structure and Dynamics

Direction of microtubule movement is an intrinsic property of the motor domains of kinesin heavy chain and Drosophila ncd protein.

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Stewart, R J, Thaler, J P, Goldstein, L S·1993·Proceedings of the National Academy of Sciences

DOI 10.1073/pnas.90.11.5209PMC PMC46685

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Abstract

The kinesin heavy chain and the ncd (non-claret disjunctional) gene product of Drosophila are microtubule-associated motor proteins related by sequence similarity within an approximately 340-aa domain. Despite the sequence similarity, the kinesin heavy chain and ncd protein move in opposite directions on microtubules. To investigate the molecular basis for direction of movement, we created a series of truncated kinesin heavy chain and ncd proteins. We found that the conserved domain of both proteins has microtubule motor activity, although the efficiency with which ATP hydrolysis is coupled to microtubule movement declines dramatically with increasing truncation. Further, the direction of movement is intrinsic to the conserved motor domains, rather than being a consequence of domain organization or adjacent sequences.

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Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase
Smith, D. B., & Johnson, K. S. (1988) · Gene · doi:10.1016/0378-1119(88)90005-4 · Cited by 5,974 · see citing works
Eukaryotic proteins expressed in Escherichia coli: an improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase.
Guan, K., & Dixon, J. (1991) · Analytical Biochemistry · doi:10.1016/0003-2697(91)90534-z · Cited by 1,735 · see citing works
Mechanochemical coupling in actomyosin energy transduction studied by in vitro movement assay
Harada, Y., Sakurada, K., Aoki, T., Thomas, D. D., & Yanagida, T. (1990) · Journal of Molecular Biology · doi:10.1016/s0022-2836(05)80060-9 · Cited by 522 · see citing works
A three-domain structure of kinesin heavy chain revealed by DNA sequence and microtubule binding analyses
Yang, J. T., Laymon, R. A., & Goldstein, L. S. B. (1989) · Cell · doi:10.1016/0092-8674(89)90692-2 · Cited by 395 · see citing works
Self-citation
The Drosophila claret segregation protein is a minus-end directed motor molecule
Walker, R. A., Salmon, E. D., & Endow, S. A. (1990) · Nature · doi:10.1038/347780a0 · Cited by 347 · see citing works
The kinesin-like ncd protein of Drosophila is a minus end-directed microtubule motor
McDonald, H. B., Stewart, R. J., & Goldstein, L. S. B. (1990) · Cell · doi:10.1016/0092-8674(90)90412-8 · Cited by 343 · see citing works
Self-citation
The Initial Phosphate Burst in ATP Hydrolysis by Myosin and Subfragment-1 as Studied by a Modified Malachite Green Method for Determination of Inorganic Phosphate
Kodama, T., Fukui, K., & Kometani, K. (1986) · The Journal of Biochemistry · doi:10.1093/oxfordjournals.jbchem.a135616 · Cited by 299 · see citing works
Mediation of meiotic and early mitotic chromosome segregation in Drosophila by a protein related to kinesin
Endow, S. A., Henikoff, S., & Soler-Niedziela, L. (1990) · Nature · doi:10.1038/345081a0 · Cited by 227 · see citing works
Evidence that the Head of Kinesin Is Sufficient for Force Generation and Motility in Vitro
Yang, J. T., Saxton, W. M., Stewart, R. J., Raff, E. C., & Goldstein, L. S. B. (1990) · Science · doi:10.1126/science.2142332 · Cited by 225 · see citing works
Self-citation
Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains.
Ingold, A. L., Cohn, S. A., & Scholey, J. M. (1988) · The Journal of Cell Biology · doi:10.1083/jcb.107.6.2657 · Cited by 175 · see citing works
Identification and characterization of a gene encoding a kinesin-like protein in Drosophila
McDonald, H. B., & Goldstein, L. S. B. (1990) · Cell · doi:10.1016/0092-8674(90)90064-l · Cited by 174 · see citing works
Self-citation
Preparation of marked microtubules for the assay of the polarity of microtubuie-based motors by fluorescence
Hyman, A. A. (1991) · Journal of Cell Science · doi:10.1242/jcs.1991.supplement_14.25 · Cited by 153 · see citing works
The kinesin superfamily: tails of functional redundancy
Goldstein, L. S. B. (1991) · Trends in Cell Biology · doi:10.1016/0962-8924(91)90036-9 · Cited by 105 · see citing works
Self-citation
Isolation of a 45-kDa fragment from the kinesin heavy chain with enhanced ATPase and microtubule-binding activities
Kuznetsov, S. A., Vaisberg, Y. A., Rothwell, S. W., Murphy, D. B., & Gelfand, V. I. (1989) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)31301-7 · Cited by 95 · see citing works
The complete sequence of Drosophila alpha-spectrin: conservation of structural domains between alpha-spectrins and alpha-actinin.
Dubreuil, R. R., Byers, T. J., Sillman, A. L., Bar‐Zvi, D., Goldstein, L. S. B., & Branton, D. (1989) · The Journal of Cell Biology · doi:10.1083/jcb.109.5.2197 · Cited by 93 · see citing works
Self-citation
The primary structure and analysis of the squid kinesin heavy chain.
Kosik, K. S., Orecchio, L. D., Schnapp, B. J., Inouye, H., & Neve, R. L. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)39764-9 · Cited by 91 · see citing works
The emerging kinesin family of microtubule motor proteins
Endow, S. A. (1991) · Trends in Biochemical Sciences · doi:10.1016/0968-0004(91)90089-e · Cited by 61 · see citing works

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Toward Intelligent Molecular Machines: Directed Motions of Biological and Artificial Molecules and Assemblies
Kinbara, K., & Aida, T. (2005) · Chemical Reviews · doi:10.1021/cr030071r · Cited by 869 · see citing works
Direct observation of single kinesin molecules moving along microtubules
Vale, R. D., Funatsu, T., Pierce, D. W., Romberg, L., Harada, Y., & Yanagida, T. (1996) · Nature · doi:10.1038/380451a0 · Cited by 711 · see citing works
The neuron-specific kinesin superfamily protein KIF1A is a uniqye monomeric motor for anterograde axonal transport of synaptic vesicle precursors
Okada, Y., Yamazaki, H., Sekine‐Aizawa, Y., & Hirokawa, N. (1995) · Cell · doi:10.1016/0092-8674(95)90538-3 · Cited by 679 · see citing works
Physical Properties Determining Self-Organization of Motors and Microtubules
Surrey, T., Nédélec, F., Leibler, S., & Karsenti, E. (2001) · Science · doi:10.1126/science.1059758 · Cited by 610 · see citing works
Force Generation by Microtubule Assembly/Disassembly in Mitosis and Related Movements
Inoué, S., & Salmon, E. D. (1995) · Molecular Biology of the Cell · doi:10.1091/mbc.6.12.1619 · Cited by 526 · see citing works
Molecular motors: structural adaptations to cellular functions
Howard, J. (1997) · Nature · doi:10.1038/39247 · Cited by 489 · see citing works
The design plan of kinesin motors
Vale, R. D., & Fletterick, R. J. (1997) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.13.1.745 · Cited by 474 · see citing works
Kinesin Takes One 8-nm Step for Each ATP That It Hydrolyzes
Coy, D. L., Wagenbach, M., & Howard, J. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.6.3667 · Cited by 390 · see citing works
Microtubules and microtubule-associated proteins
Mandelkow, E., & Mandelkow, E.-M. (1995) · Current Opinion in Cell Biology · doi:10.1016/0955-0674(95)80047-6 · Cited by 458 · see citing works
Identification and partial characterization of mitotic centromere-associated kinesin, a kinesin-related protein that associates with centromeres during mitosis.
Wordeman, L. G., & Mitchison, T. J. (1995) · The Journal of Cell Biology · doi:10.1083/jcb.128.1.95 · Cited by 381 · see citing works
Kinesin and dynein superfamily proteins in organelle transport and cell division
Hirokawa, N., Noda, Y., & Okada, Y. (1998) · Current Opinion in Cell Biology · doi:10.1016/s0955-0674(98)80087-2 · Cited by 364 · see citing works
Coupling of kinesin steps to ATP hydrolysis
Hua, W., Young, E. C., Fleming, M. L., & Gelles, J. (1997) · Nature · doi:10.1038/41118 · Cited by 358 · see citing works
Crystal structure of the motor domain of the kinesin-related motor ncd
Sablin, E. P., Kull, F. J., Cooke, R., Vale, R. D., & Fletterick, R. J. (1996) · Nature · doi:10.1038/380555a0 · Cited by 353 · see citing works
Evidence for alternating head catalysis by kinesin during microtubule-stimulated ATP hydrolysis.
Hackney, D. D. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.15.6865 · Cited by 351 · see citing works
The Directional Preference of Kinesin Motors Is Specified by an Element outside of the Motor Catalytic Domain
Case, R. B., Pierce, D. W., Hom-Booher, N., Hart, C. L., & Vale, R. D. (1997) · Cell · doi:10.1016/s0092-8674(00)80360-8 · Cited by 348 · see citing works
Light-Controlled Molecular Shuttles Made from Motor Proteins Carrying Cargo on Engineered Surfaces
Hess, H., Clemmens, J., Qin, D., Howard, J., & Viola Vogel, B. (2001) · Nano Letters · doi:10.1021/nl015521e · Cited by 334 · see citing works
Kinesin׳s tail domain is an inhibitory regulator of the motor domain
Coy, D. L., Hancock, W. O., Wagenbach, M., & Howard, J. (1999) · Nature Cell Biology · doi:10.1038/13001 · Cited by 329 · see citing works
Processivity of the Motor Protein Kinesin Requires Two Heads
Hancock, W. O., & Howard, J. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.140.6.1395 · Cited by 296 · see citing works
The Road Less Traveled: Emerging Principles of Kinesin Motor Utilization
Goldstein, L. S. B., & Philp, A. V. (1999) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.15.1.141 · Cited by 269 · see citing works
Self-citation
Light Chain– dependent Regulation of Kinesin's Interaction with Microtubules
Verhey, K. J., Lizotte, D. L., Abramson, T., Barenboim, L., Schnapp, B. J., & Rapoport, T. A. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.143.4.1053 · Cited by 266 · see citing works
“Inhibition of motor activity by the HC tail is in agreement with previous reports that the motor domain alone has higher MT-stimulated ATPase activity than intact kinesin (Kuznetsov et al., 1989; Gilbert and Johnson, 1993; Stewart et al., 1993; Huang and Hackney, 1994; discussed in Hackney, 1996).”
BackgroundResult
Yeast Kar3 is a minus‐end microtubule motor protein that destabilizes microtubules preferentially at the minus ends.
Endow, S. A., KANG, S., Satterwhite, L. L., Rose, M. D., Skeen, V. P., & Salmon, E. D. (1994) · The EMBO Journal · doi:10.1002/j.1460-2075.1994.tb06561.x · Cited by 263 · see citing works
Switches, latches, and amplifiers: common themes of G proteins and molecular motors.
Vale, R. D. (1996) · The Journal of Cell Biology · doi:10.1083/jcb.135.2.291 · Cited by 258 · see citing works
Pathway of processive ATP hydrolysis by kinesin
Gilbert, S. P., Webb, M. R., Brune, M., & Johnson, K. A. (1995) · Nature · doi:10.1038/373671a0 · Cited by 250 · see citing works
Anastral meiotic spindle morphogenesis: role of the non-claret disjunctional kinesin-like protein.
Matthies, H. J. G., McDonald, H. B., Goldstein, L. S. B., & Theurkauf, W. E. (1996) · The Journal of Cell Biology · doi:10.1083/jcb.134.2.455 · Cited by 247 · see citing works
Self-citation
The Movement of Kinesin Along Microtubules
Howard, J. (1996) · Annual Review of Physiology · doi:10.1146/annurev.ph.58.030196.003415 · Cited by 244 · see citing works
Kinesin’s processivity results from mechanical and chemical coordination between the ATP hydrolysis cycles of the two motor domains
Hancock, W. O., & Howard, J. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.23.13147 · Cited by 243 · see citing works
Two-Dimensional Tracking of ncd Motility by Back Focal Plane Interferometry
Allersma, M. W., Gittes, F., deCastro, M. J., Stewart, R. J., & Schmidt, C. F. (1998) · Biophysical Journal · doi:10.1016/s0006-3495(98)74031-7 · Cited by 235 · see citing works
Self-citation
Reciprocal localization of Nod and kinesin fusion proteins indicates microtubule polarity in the Drosophila oocyte, epithelium, neuron and muscle
Clark, I. E., Jan, L. Y., & Jan, Y. N. (1997) · Development · doi:10.1242/dev.124.2.461 · Cited by 223 · see citing works
The force generated by a single kinesin molecule against an elastic load.
Meyhöfer, E., & Howard, J. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.2.574 · Cited by 213 · see citing works
Antibodies to the kinesin motor domain and CENP-E inhibit microtubule depolymerization-dependent motion of chromosomes in vitro.
Lombillo, V. A., Nislow, C., Yen, T. J., Gelfand, V. I., & McIntosh, J. R. (1995) · The Journal of Cell Biology · doi:10.1083/jcb.128.1.107 · Cited by 213 · see citing works
Reversal in the direction of movement of a molecular motor
Henningsen, U., & Schliwa, M. (1997) · Nature · doi:10.1038/38022 · Cited by 204 · see citing works
Bidirectional cooperative motion of molecular motors
Badoual, M., Jülicher, F., & Prost, J. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.102692399 · Cited by 186 · see citing works
Kinesin light chain -independent function of the Kinesin heavy chain in cytoplasmic streaming and posterior localisation in the Drosophila oocyte
Palacios, I. M., & Johnston, D. S. (2002) · Development · doi:10.1242/dev.00119 · Cited by 184 · see citing works
Minus-end-directed motion of kinesin–coated microspheres driven by microtubule depolymerization
Lombillo, V. A., Stewart, R. J., & McIntosh, J. R. (1995) · Nature · doi:10.1038/373161a0 · Cited by 178 · see citing works
Self-citation
Drosophila kinesin minimal motor domain expressed in Escherichia coli. Purification and kinetic characterization
Huang, T. G., & Hackney, D. D. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)34033-4 · Cited by 174 · see citing works
Failure of a single-headed kinesin to track parallel to microtubule protofilaments
Berliner, E., Young, E. C., Anderson, K., Mahtani, H. K., & Gelles, J. (1995) · Nature · doi:10.1038/373718a0 · Cited by 174 · see citing works
Kinesin proteins: A phylum of motors for microtubule‐based motility
Moore, J. D., & Endow, S. A. (1996) · BioEssays · doi:10.1002/bies.950180308 · Cited by 168 · see citing works
Interaction of Kinesin Motor Domains with α- and β-Tubulin Subunits at a Tau-independent Binding Site
Larcher, J.-C., Boucher, D., Lazereg, S., Gros, F., & Denoulet, P. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.36.22117 · Cited by 163 · see citing works
Formation of the Compact Confomer of Kinesin Requires a COOH-terminal Heavy Chain Domain and Inhibits Microtubule-stimulated ATPase Activity
Stock, M. F., Guerrero, J., Cobb, B. A., Eggers, C. T., Huang, T.-G., Li, X., & Hackney, D. D. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.21.14617 · Cited by 159 · see citing works
Spontaneous X chromosome MI and MII nondisjunction events in Drosophila melanogaster oocytes have different recombinational histories
Koehler, K. E., Boulton, C. L., Collins, H. E., French, R. L., Herman, K. C., Lacefield, S., Madden, L. D., Schuetz, C. D., & Hawley, R. S. (1996) · Nature Genetics · doi:10.1038/ng1296-406 · Cited by 158 · see citing works
A Model for the Microtubule-Ncd Motor Protein Complex Obtained by Cryo-Electron Microscopy and Image Analysis
Sosa, H., Dias, D. P., Hoenger, A., Whittaker, M., Kubalek, E. W., Sablin, E. P., Fletterick, R. J., Vale, R. D., & Milligan, R. A. (1997) · Cell · doi:10.1016/s0092-8674(00)80330-x · Cited by 157 · see citing works
Role of the Kinesin Neck Region in Processive Microtubule-based Motility
Romberg, L., Pierce, D. W., & Vale, R. D. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.140.6.1407 · Cited by 154 · see citing works
Molecular analysis of the γ heavy chain of Chlamydomonas flagellar outerarm dynein
Wilkerson, C. G., King, S. M., & Witman, G. B. (1994) · Journal of Cell Science · doi:10.1242/jcs.107.3.497 · Cited by 144 · see citing works
A Kinesin-Like Protein, KatAp, in the Cells of Arabidopsis and Other Plants
Liu, B., Cyr, R. J., & Palevitz, B. A. (1996) · The Plant Cell · doi:10.1105/tpc.8.1.119 · Cited by 141 · see citing works
Molecular shuttles: directed motion of microtubules along nanoscale kinesin tracks
Dennis, J. R., Howard, J., & Viola Vogel, B. (1999) · Nanotechnology · doi:10.1088/0957-4484/10/3/302 · Cited by 140 · see citing works
The Nature of Nanotechnology
Schmid, G. (2010) · Nanotechnology · doi:10.1002/9783527628155.nanotech002 · Cited by 139 · see citing works
Three-dimensional cryoelectron microscopy of dimeric kinesin and ncd motor domains on microtubules.
Hirose, K., Lockhart, A., Cross, R. A., & Amos, L. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.18.9539 · Cited by 131 · see citing works
Conformational changes during kinesin motility
Schief, W. R., & Howard, J. (2001) · Current Opinion in Cell Biology · doi:10.1016/s0955-0674(00)00169-1 · Cited by 131 · see citing works
Mitochondrial Association of a Plus End–Directed Microtubule Motor Expressed during Mitosis in Drosophila
Pereira, A., Dalby, B., Stewart, R. J., Doxsey, S., & Goldstein, L. S. B. (1997) · The Journal of Cell Biology · doi:10.1083/jcb.136.5.1081 · Cited by 121 · see citing works
Self-citation
Localization of the Kar3 kinesin heavy chain-related protein requires the Cik1 interacting protein
Page, B. D., Satterwhite, L. L., Rose, M. D., & Snyder, M. P. (1994) · The Journal of Cell Biology · doi:10.1083/jcb.124.4.507 · Cited by 118 · see citing works
Hybrid Hydrogels Cross-Linked by Genetically Engineered Coiled-Coil Block Proteins
Wang, C., Kopec̆ek, J., & Stewart, R. J. (2001) · Biomacromolecules · doi:10.1021/bm0155322 · Cited by 110 · see citing works
Self-citation
A lever-arm rotation drives motility of the minus-end-directed kinesin Ncd
Endres, N., Yoshioka, C., Milligan, R. A., & Vale, R. D. (2005) · Nature · doi:10.1038/nature04320 · Cited by 110 · see citing works
Mutations in the SUP-PF-1 locus of Chlamydomonas reinhardtii identify a regulatory domain in the beta-dynein heavy chain.
Porter, M. E., Knott, J. A., Gardner, L. C., Mitchell, D. R., & Dutcher, S. K. (1994) · The Journal of Cell Biology · doi:10.1083/jcb.126.6.1495 · Cited by 109 · see citing works
Molecular phylogeny of the kinesin family of microtubule motor proteins
Goodson, H. V., KANG, S., & Endow, S. A. (1994) · Journal of Cell Science · doi:10.1242/jcs.107.7.1875 · Cited by 106 · see citing works
A Marine Natural Product Inhibitor of Kinesin Motors
Sakowicz, R., Berdelis, M. S., Ray, K., Blackburn, C. L., Hopmann, C., Faulkner, D. J., & Goldstein, L. S. B. (1998) · Science · doi:10.1126/science.280.5361.292 · Cited by 99 · see citing works
Self-citation
A plant kinesin heavy chain‐like protein is a calmodulin‐binding protein
Reddy, A. S. N., Narasimhulu, S. B., Safadi, F., & Golovkin, M. (1996) · The Plant Journal · doi:10.1046/j.1365-313x.1996.10010009.x · Cited by 93 · see citing works
Force generation by kinesin and myosin cytoskeletal motor proteins
Kull, F. J., & Endow, S. A. (2012) · Journal of Cell Science · doi:10.1242/jcs.103911 · Cited by 92 · see citing works
Determinants of molecular motor directionality
Endow, S. A. (1999) · Nature Cell Biology · doi:10.1038/14113 · Cited by 92 · see citing works
Rotation of the stalk/neck and one head in a new crystal structure of the kinesin motor protein, Ncd
Yun, M. K., Bronner, C. E., Park, C., Cha, S., Park, H., & Endow, S. A. (2003) · The EMBO Journal · doi:10.1093/emboj/cdg531 · Cited by 90 · see citing works
Drosophila kinesin motor domain extending to amino acid position 392 is dimeric when expressed in Escherichia coli.
Huang, T. G., Suhan, J. P., & Hackney, D. D. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)34034-6 · Cited by 90 · see citing works
Self-assembly driven by molecular motors
Hess, H. (2006) · Soft Matter · doi:10.1039/b518281f · Cited by 88 · see citing works
Tubulin depolymerization may be an ancient biological motor
McIntosh, J. R., Volkov, V. A., Ataullakhanov, F. I., & Grishchuk, E. L. (2010) · Journal of Cell Science · doi:10.1242/jcs.067611 · Cited by 87 · see citing works
Demonstration of Coiled-Coil Interactions within the Kinesin Neck Region Using Synthetic Peptides
Tripet, B., Vale, R. D., & Hodges, R. S. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.14.8946 · Cited by 87 · see citing works
Effect of temperature on kinesin‐driven microtubule gliding and kinesin ATPase activity
Böhm, K. J., Stracke, R., Baum, M., Zieren, M., & Unger, E. (2000) · FEBS Letters · doi:10.1016/s0014-5793(99)01757-3 · Cited by 84 · see citing works
Pre-Steady-State Kinetics of the Microtubule.cntdot.Kinesin ATPase
Gilbert, S. P., & Johnson, K. A. (1994) · Biochemistry · doi:10.1021/bi00173a044 · Cited by 84 · see citing works
Three-dimensional structure of functional motor proteins on microtubules
Arnal, I., Metoz, F., DeBonis, S., & Wade, R. H. (1996) · Current Biology · doi:10.1016/s0960-9822(02)70712-4 · Cited by 84 · see citing works
The Sliding Filament Model
Cooke, R. (2004) · The Journal of General Physiology · doi:10.1085/jgp.200409089 · Cited by 80 · see citing works
SPEEDING UP KINESIN‐DRIVEN MICROTUBULE GLIDING IN VITRO BY VARIATION OF COFACTOR COMPOSITION AND PHYSICOCHEMICAL PARAMETERS*
Böhm, K., Stracke, R., & Unger, E. (2000) · Cell Biology International · doi:10.1006/cbir.1999.0515 · Cited by 77 · see citing works
Influence of the kinesin neck domain on dimerization and ATPase kinetics
Jiang, W., Stock, M. F., Li, X., & Hackney, D. D. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.12.7626 · Cited by 75 · see citing works
Structures of kinesin and kinesin–microtubule interactions
Mandelkow�, E., & Hoenger, A. (1999) · Current Opinion in Cell Biology · doi:10.1016/s0955-0674(99)80005-2 · Cited by 74 · see citing works
The mitotic kinesin CENP-E is a processive transport motor
Yardimci, H., van Duffelen, M., Mao, Y., Rosenfeld, S. S., & Selvin, P. R. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0711314105 · Cited by 72 · see citing works
Microtubule movement by a biotinated kinesin bound to streptavidin-coated surface.
Berliner, E., Mahtani, H. K., Karki, S., Chu, L. F., Cronan, J. E., & Gelles, J. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)37238-1 · Cited by 72 · see citing works
The rate-limiting step in microtubule-stimulated ATP hydrolysis by dimeric kinesin head domains occurs while bound to the microtubule
Hackney, D. D. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)34035-8 · Cited by 68 · see citing works
Characterization of the KLP68D kinesin-like protein in Drosophila: possible roles in axonal transport.
Pesavento, P. A., Stewart, R. J., & Goldstein, L. S. B. (1994) · The Journal of Cell Biology · doi:10.1083/jcb.127.4.1041 · Cited by 66 · see citing works
Self-citation
Two modes of microtubule sliding driven by cytoplasmic dynein
Shima, T., Kon, T., Imamula, K., Ohkura, R., & Sutoh, K. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0606794103 · Cited by 64 · see citing works
Origins of reversed directionality in the ncd molecular motor.
Lockhart, A., & Cross, R. A. (1994) · The EMBO Journal · doi:10.1002/j.1460-2075.1994.tb06317.x · Cited by 64 · see citing works
Mitotic HeLa cells contain a CENP‐E‐associated minus end‐directed microtubule motor.
Thrower, D., Jordan, M. A., Schaar, B. T., Yen, T. J., & Wilson, L. (1995) · The EMBO Journal · doi:10.1002/j.1460-2075.1995.tb07073.x · Cited by 64 · see citing works
A torque component in the kinesin-1 power stroke
Yajima, J., & Cross, R. A. (2005) · Nature Chemical Biology · doi:10.1038/nchembio740 · Cited by 61 · see citing works
Can polymer brushes induce motion of nano-objects?
Prokhorova, S. A., Kopyshev, A., Ramakrishnan, A., Zhang, H., & Rühe, J. (2003) · Nanotechnology · doi:10.1088/0957-4484/14/10/306 · Cited by 61 · see citing works
Movements of truncated kinesin fragments with a short or an artificial flexible neck
Inoue, Y., Toyoshima, Y. Y., Iwane, A. H., Morimoto, S., Higuchi, H., & Yanagida, T. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.14.7275 · Cited by 61 · see citing works
Motor domains of kinesin and ncd interact with microtubule protofilaments with the same binding geometry
Hoenger, A., & Milligan, R. A. (1997) · Journal of Molecular Biology · doi:10.1006/jmbi.1996.0757 · Cited by 60 · see citing works
Probing the Kinesin-Microtubule Interaction
Tucker, C., & Goldstein, L. S. B. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.14.9481 · Cited by 57 · see citing works
Self-citation
Equilibrium studies of kinesin-nucleotide intermediates
Rosenfeld, S. S., Rener, B., Correia, J. J., Mayo, M. S., & Cheung, H. C. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.16.9473 · Cited by 56 · see citing works
Microtubules and Microtubule Motors: Mechanisms of Regulation
Thaler, C. D., & Haimo, L. T. (1996) · International review of cytology · doi:10.1016/s0074-7696(08)62388-8 · Cited by 56 · see citing works
Orphan Kinesin NOD Lacks Motile Properties But Does Possess a Microtubule-stimulated ATPase Activity
Matthies, H. J. G., Baskin, R. J., & Hawley, R. S. (2001) · Molecular Biology of the Cell · doi:10.1091/mbc.12.12.4000 · Cited by 54 · see citing works
Equilibrium Binding Studies of Non-claret Disjunctional Protein (Ncd) Reveal Cooperative Interactions between the Motor Domains
Foster, K. A., Correia, J. J., & Gilbert, S. P. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.52.35307 · Cited by 53 · see citing works
Nucleotide-dependent structural changes in dimeric NCD molecules complexed to microtubules 1 1Edited by R. Huber
Hirose, K., Cross, R. A., & Amos, L. (1998) · Journal of Molecular Biology · doi:10.1006/jmbi.1998.1709 · Cited by 53 · see citing works
Microtubule Dynamics and Chromosome Movement
McIntosh, J. R., Lombillo, V. A., Nislow, C., & Vaisberg, E. A. (1995) · Springer eBooks · doi:10.1007/978-3-642-79482-7_1 · Cited by 52 · see citing works
A Minimal Motor Domain from Chicken Skeletal Muscle Myosin
Waller, G. S., Ouyang, G., Swafford, J., Vibert, P. J., & Lowey, S. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.25.15348 · Cited by 50 · see citing works
Importance of a flexible hinge near the motor domain in kinesin‐driven motility
Grummt, M., Woehlke, G., Henningsen, U., Fuchs, S., Schleicher, M., & Schliwa, M. (1998) · The EMBO Journal · doi:10.1093/emboj/17.19.5536 · Cited by 48 · see citing works
Motor activity and mitotic spindle localization of the Drosophila kinesin-like protein KLP61F.
Barton, N. R., Pereira, A., & Goldstein, L. S. B. (1995) · Molecular Biology of the Cell · doi:10.1091/mbc.6.11.1563 · Cited by 45 · see citing works
Self-citation
Nucleotide-dependent conformations of the kinesin dimer interacting with microtubules
Arnal, I., & Wade, R. H. (1998) · Structure · doi:10.1016/s0969-2126(98)00005-7 · Cited by 45 · see citing works
Sedimentation studies on the kinesin motor domain constructs K401, K366, and K341
Correia, J. J., Gilbert, S. P., Moyer, M. L., & Johnson, K. A. (1995) · Biochemistry · doi:10.1021/bi00014a047 · Cited by 44 · see citing works
ncd and kinesin motor domains interact with both alpha- and beta-tubulin.
Walker, R. A. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.13.5960 · Cited by 40 · see citing works
Mutational Analysis of Motor Proteins
Sweeney, H. L., & Holzbaur, E. L. F. (1996) · Annual Review of Physiology · doi:10.1146/annurev.ph.58.030196.003535 · Cited by 40 · see citing works
Kinesin-Related Proteins in Plant Cytokinesis
Liu, B., & Lee, Y. J. (2001) · Journal of Plant Growth Regulation · doi:10.1007/s003440010012 · Cited by 39 · see citing works
Searching for kinesin's mechanical amplifier
Vale, R. D., Case, R., Sablin, E. P., Hart, C., & Fletterick, R. J. (2000) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.2000.0586 · Cited by 36 · see citing works
The Correlation Ratchet: A Novel Mechanism for Generating Directed Motion by ATP Hydrolysis
Peskin, C. S., Ermentrout, B., & OSTER, G. F. (1994) · Springer eBooks · doi:10.1007/978-1-4613-8425-0_26 · Cited by 35 · see citing works
Nucleotide-Dependent Movements of the Kinesin Motor Domain Predicted by Simulated Annealing
Wriggers, W. R., & Schulten, K. J. (1998) · Biophysical Journal · doi:10.1016/s0006-3495(98)77555-1 · Cited by 35 · see citing works
Structure and dynamics of molecular motors
Amos, L., & Cross, R. A. (1997) · Current Opinion in Structural Biology · doi:10.1016/s0959-440x(97)80032-2 · Cited by 31 · see citing works

An N-terminal truncation of the ncd motor protein supports diffusional movement of microtubules in motility assays
Chandra, R., Endow, S. A., & Salmon, E. D. (1993) · Journal of Cell Science · doi:10.1242/jcs.104.3.899 · Cited by 37 · see citing works
The kinesin-like ncd protein of Drosophila is a minus end-directed microtubule motor
McDonald, H. B., Stewart, R. J., & Goldstein, L. S. B. (1990) · Cell · doi:10.1016/0092-8674(90)90412-8 · Cited by 343 · see citing works
Self-citation
Structural and functional domains of the Drosophila ncd microtubule motor protein.
Chandra, R., Salmon, E. D., Erickson, H., Lockhart, A., & Endow, S. A. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)52971-9 · Cited by 157 · see citing works
Motor domains of kinesin and ncd interact with microtubule protofilaments with the same binding geometry
Hoenger, A., & Milligan, R. A. (1997) · Journal of Molecular Biology · doi:10.1006/jmbi.1996.0757 · Cited by 60 · see citing works
Reversal in the direction of movement of a molecular motor
Henningsen, U., & Schliwa, M. (1997) · Nature · doi:10.1038/38022 · Cited by 204 · see citing works

Authors: Stewart, R. J., Thaler, J. P., & Goldstein, L. S.
Affiliations: Harvard University (US)
ORCID: Goldstein, L S
Venue: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 1993-06
Volume: 90
Issue: 11
Pages: 5209-5213
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.90.11.5209
PMC: PMC46685
OpenAlex: W2056695965
Cited by: 145
References: 17
Citations by year: 2026: 2 · 2025: 3 · 2024: 2 · 2021: 1 · 2020: 1 · 2019: 2 · 2016: 1 · 2012: 2
FWCI: 7.40
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Locations: Proceedings of the National Academy of Sciences (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Kidney Cyst Diseases
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
Keywords: kinesin heavy chain, microtubule motor proteins, motor domain, ATP hydrolysis, truncated protein, Drosophila, sequence similarity
MeSH terms: Animals, Drosophila, Glutathione Transferase, Microtubule Proteins, Microtubules, Plasmids, Recombinant Fusion Proteins, Spectrin, Restriction Mapping, Kinesins, Drosophila Proteins, Macromolecular Substances

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Direction of microtubule movement is an intrinsic property of the motor domains of kinesin heavy chain and Drosophila ncd protein.

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