The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
Mitogen-regulated Ca2+ current of T lymphocytes is activated by depletion of intracellular Ca2+ stores.
Open accessAbstract
Stimulated influx of Ca2+ across the plasma membrane of T lymphocytes is an essential triggering signal for T-cell activation by antigen. Regulation of the T-cell Ca2+ conductance is not understood; conflicting evidence supports direct activation by inositol 1,4,5-trisphosphate (IP3) or by a signal generated by the depletion of intracellular Ca2+ stores. We have used the perforated-patch recording technique to compare the biophysical properties of Ca2+ currents activated by T-cell receptor stimulation and by thapsigargin, a Ca(2+)-ATPase inhibitor that depletes intracellular stores without generating IP3. Both currents are blocked by Ni2+, are inwardly rectifying, are highly Ca(2+)-selective, and exhibit voltage-independent gating with a unitary chord conductance of approximately 24 fS in isotonic Ca2+. Fluctuation analysis suggests that the underlying Ca2+ transporter is a channel rather than an iron carrier. Thus, in terms of ion permeation, gating, and unitary conductance, the Ca2+ current activated by thapsigargin is indistinguishable from the elicited by crosslinking of T-cell receptors. Moreover, the unitary Ca2+ conductance is > 100-fold smaller than that of previously described IP3-gated, Ca(2+)-permeable channels in T cells [Kuno, M. & Gardner, P. (1987) Nature (London) 326, 301-304]. These results demonstrate that mitogen-activated Ca2+ influx is controlled by the state of intracellular Ca2+ stores rather than by the direct action of IP3 on Ca2+ channels in the plasma membrane.
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Down-Regulation of Na+/K+ATPase Activity by Human Parvovirus B19 Capsid Protein VP1
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Regulation of the Voltage Gated K+ Channel Kv1.3 by Recombinant Human Klotho Protein
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Inositol phosphates and cell signalling
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Depletion of intracellular calcium stores activates a calcium current in mast cells
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Capacitative calcium entry revisited
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Thapsigargin inhibits the sarcoplasmic or endoplasmic reticulum Ca-ATPase family of calcium pumps.
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Contingent Genetic Regulatory Events in T Lymphocyte Activation
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Cited by
Store-Operated Calcium Channels
Parekh, A. B., & Putney, J. W. (2005) · Physiological Reviews · doi:10.1152/physrev.00057.2003 · Cited by 2,089
Calcium signalling in lymphocyte activation and disease
Feske, S. (2007) · Nature Reviews Immunology · doi:10.1038/nri2152 · Cited by 1,043
Calcium signaling mechanisms in T lymphocytes.
Lewis, R. S. (2001) · Annual Review of Immunology · doi:10.1146/annurev.immunol.19.1.497 · Cited by 860
Self-citationMolecular Basis of Calcium Signaling in Lymphocytes: STIM and ORAI
Hogan, P. G., Lewis, R. S., & Rao, A. (2010) · Annual Review of Immunology · doi:10.1146/annurev.immunol.021908.132550 · Cited by 760
Self-citationTu, H., Nelson, O., Bezprozvanny, A., Wang, Z., Lee, S.-F., Hao, Y.-H., Serneels, L., De Strooper, B., Yu, G., & Bezprozvanny, I. (2006) · Cell · doi:10.1016/j.cell.2006.06.059 · Cited by 616
Metadata
- Authors
- Zweifach, A., & Lewis, R. S.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1993-07
- Volume
- 90
- Issue
- 13
- Pages
- 6295-6299
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2076882136
- Cited by
- 760
- References
- 31
- FWCI
- 23.23
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Zweifach, A., & Lewis, R. S. (1993). Mitogen-regulated Ca2+ current of T lymphocytes is activated by depletion of intracellular Ca2+ stores. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.90.13.6295
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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