DNA damage and repair in telomeres: relation to aging
Open accessAbstract
We have established a method for the detection of DNA damage and its repair in human telomeres, the natural ends of chromosomes which are necessary for replication and critical for chromosomal stability. We find that ultraviolet light-induced pyrimidine dimers in telomeric DNA are repaired less efficiently than endogenous genes but more efficiently than inactive, noncoding regions. We have also measured telomeric length, telomeric DNA damage, and its repair in relation to the progression of aging. Telomeres are shorter in fibroblasts from an old donor compared to fibroblasts from a young donor, shortest in cells from a patient with the progeroid disorder Werner syndrome, and relatively long in fibroblasts from a patient with Alzheimer disease. Telomeric DNA repair efficiency is lower in cells from an old donor than in cells from a young donor, normal in Alzheimer cells, and slightly lower in Werner cells. It is possible that this decline in telomeric repair with aging is of functional significance to an age-related decline in genomic stability.
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- Authors
- Kruk, P. A., Rampino, N. J., & Bohr, V. A.
- Affiliations
- National Institutes of Health (US)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1995-01-03
- Volume
- 92
- Issue
- 1
- Pages
- 258-262
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC42857
- OpenAlex
- W2072908461
- Cited by
- 333
- References
- 35
- Citations by year
- 2026: 4 · 2025: 5 · 2024: 9 · 2023: 9 · 2022: 17 · 2021: 11 · 2020: 5 · 2019: 10 · 2018: 3 · 2017: 13 · 2016: 8 · 2015: 8 · 2014: 10 · 2013: 3 · 2012: 12
- FWCI
- 8.39
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Telomeres, Telomerase, and Senescence
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Keywords
- telomeres, DNA damage, DNA repair, aging, pyrimidine dimers, ultraviolet radiation, telomere length, Werner syndrome, progeroid disorder, Alzheimer's disease, fibroblasts, genome stability, chromosome stability
- MeSH terms
- Aged, Alzheimer Disease, Base Sequence, Cell Line, Child, DNA, DNA Damage, DNA Repair, Fibroblasts, Humans, Infant, Newborn, Kinetics, Molecular Sequence Data, Pyrimidine Dimers, Ultraviolet Rays, Werner Syndrome, Blotting, Southern, Restriction Mapping, Telomere
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