The DOI matches its Crossref record; 18 of the 18 references it cites were found in a registry or an index.
Green fluorescent protein as a vital marker and reporter of gene expression in Drosophila.
Open accessAbstract
We have used the green fluorescent protein (GFP) from the jellyfish Aequorea victoria as a vital marker/reporter in Drosophila melanogaster. Transgenic flies were generated in which GFP was expressed under the transcriptional control of the yeast upstream activating sequence that is recognized by GAL4. These flies were crossed to several GAL4 enhancer trap lines, and expression of GFP was monitored in a variety of tissues during development using confocal microscopy. Here, we show that GFP could be detected in freshly dissected ovaries, imaginal discs, and the larval nervous system without prior fixation or the addition of substrates or antibodies. We also show that expression of GFP could be monitored in intact living embryos and larvae and in cultured egg chambers, allowing us to visualize dynamic changes in gene expression during real time.
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Cites
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Tauopathy in Drosophila : Neurodegeneration Without Neurofibrillary Tangles
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Verstreken, P., Ly, C. V., Venken, K. J. T., Koh, T.-W., Zhou, Y., & Bellen, H. J. (2005) · Neuron · doi:10.1016/j.neuron.2005.06.018 · Cited by 841
ATAXIN-1 Interacts with the Repressor Capicua in Its Native Complex to Cause SCA1 Neuropathology
Lam, Y. C., Bowman, A. B., Jafar-Nejad, P., Lim, J., Richman, R., Fryer, J. D., Hyun, E. D., Duvick, L. A., Orr, H. T., Botas, J., et al. (2006) · Cell · doi:10.1016/j.cell.2006.11.038 · Cited by 326
Morrow, G., Samson, M., Michaud, S., & M. Tanguay, R. (2004) · The FASEB Journal · doi:10.1096/fj.03-0860fje · Cited by 288
Role for VGLUT2 in selective vulnerability of midbrain dopamine neurons
Steinkellner, T., Zell, V., Farino, Z. J., Sonders, M. S., Villeneuve, M., Freyberg, R. J., Przedborski, S., Lu, W., Freyberg, Z., & Hnasko, T. S. (2018) · Journal of Clinical Investigation · doi:10.1172/jci95795 · Cited by 102
Metadata
- Authors
- Yeh, E., Gustafson, K., & Boulianne, G. L.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1995-07-18
- Volume
- 92
- Issue
- 15
- Pages
- 7036-7040
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2080458091
- Cited by
- 286
- References
- 18
- FWCI
- 15.20
- Type
- journal article
- Language
- en
- Open access
- green
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- Yeh, E., Gustafson, K., & Boulianne, G. L. (1995). Green fluorescent protein as a vital marker and reporter of gene expression in Drosophila. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.92.15.7036
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