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Cited by 286

The DOI matches its Crossref record; 18 of the 18 references it cites were found in a registry or an index.

Green fluorescent protein as a vital marker and reporter of gene expression in Drosophila.

Open access
Yeh, E, Gustafson, K, Boulianne, G L·1995·Proceedings of the National Academy of Sciences

Abstract

We have used the green fluorescent protein (GFP) from the jellyfish Aequorea victoria as a vital marker/reporter in Drosophila melanogaster. Transgenic flies were generated in which GFP was expressed under the transcriptional control of the yeast upstream activating sequence that is recognized by GAL4. These flies were crossed to several GAL4 enhancer trap lines, and expression of GFP was monitored in a variety of tissues during development using confocal microscopy. Here, we show that GFP could be detected in freshly dissected ovaries, imaginal discs, and the larval nervous system without prior fixation or the addition of substrates or antibodies. We also show that expression of GFP could be monitored in intact living embryos and larvae and in cultured egg chambers, allowing us to visualize dynamic changes in gene expression during real time.

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Crossref · 10.1073/pnas.92.15.7036

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Metadata

Authors
Yeh, E., Gustafson, K., & Boulianne, G. L.
Venue
Proceedings of the National Academy of Sciences
Published
1995-07-18
Volume
92
Issue
15
Pages
7036-7040
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2080458091
Cited by
286
References
18
FWCI
15.20
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Green fluorescent protein as a vital marker and reporter of gene expression in Drosophila. https://app.cochat.ai/papers/10.1073/pnas.92.15.7036
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Yeh, E., Gustafson, K., & Boulianne, G. L. (1995). Green fluorescent protein as a vital marker and reporter of gene expression in Drosophila. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.92.15.7036

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.