The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Elevation of intracellular calcium in smooth muscle causes endothelial cell generation of NO in arterioles
Open accessAbstract
It is well known that vascular smooth muscle tone can be modulated by signals arising in the endothelium (e.g., endothelium-derived relaxing factor, endothelium-derived hyperpolarizing factor, and prostaglandins). Here we show that during vasoconstriction a signal can originate in smooth muscle cells and act on the endothelium to cause synthesis of endothelium-derived relaxing factor. We studied responses to two vasoconstrictors (phenylephrine and KCl) that act by initiating a rise in smooth muscle cell intracellular Ca 2+ concentration ([Ca 2+ ] i ) and exert little or no direct effect on the endothelium. Fluo-3 was used as a Ca 2+ indicator in either smooth muscle or endothelial cells of arterioles from the hamster cheek pouch. Phenylephrine and KCl caused the expected rise in smooth muscle cell [Ca 2+ ] i that was accompanied by an elevation in endothelial cell [Ca 2+ ] i . The rise in endothelial cell [Ca 2+ ] i was followed by increased synthesis of NO, as evidenced by an enhancement of the vasoconstriction induced by both agents after blockade of NO synthesis. The molecule involved in signal transmission from smooth muscle to endothelium is as yet unknown. However, given that myoendothelial cell junctions are frequent in these vessels, we hypothesize that the rise in smooth muscle cell Ca 2+ generates a diffusion gradient that drives Ca 2+ through myoendothelial cell junctions and into the endothelial cells, thereby initiating the synthesis of NO.
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A new generation of Ca2+ indicators with greatly improved fluorescence properties.
Grynkiewicz, G., Poenie, M., & Tsien, R. Y. (1985) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)83641-4 · Cited by 21,607
Polazzi, E., Mengoni, I., Caprini, M., Peña-Altamira, E., Kurtys, E., & Monti, B. (2013) · Neurosignals · doi:10.1159/000337115 · Cited by 8,994
Identification of Epoxyeicosatrienoic Acids as Endothelium-Derived Hyperpolarizing Factors
Campbell, W. B., Gebremedhin, D., Pratt, P. F., & Harder, D. R. (1996) · Circulation Research · doi:10.1161/01.res.78.3.415 · Cited by 1,221
Range of Messenger Action of Calcium Ion and Inositol 1,4,5-Trisphosphate
Allbritton, N. L., Meyer, T., & Stryer, L. (1992) · Science · doi:10.1126/science.1465619 · Cited by 1,148
Mechanisms and function of intercellular calcium signaling
Sanderson, M. J., Charles, A. C., Boitano, S., & Dirksen, E. R. (1994) · Molecular and Cellular Endocrinology · doi:10.1016/0303-7207(94)90136-8 · Cited by 305
Little, T. L., Beyer, E. C., & Duling, B. R. (1995) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.1995.268.2.h729 · Cited by 292
Self-citationAyajiki, K., Kindermann, M., Hecker, M., Fleming, I., & Busse, R. (1996) · Circulation Research · doi:10.1161/01.res.78.5.750 · Cited by 285
Little, T. L., Xia, J., & Duling, B. R. (1995) · Circulation Research · doi:10.1161/01.res.76.3.498 · Cited by 261
Self-citationJones, C. J., DeFily, D. V., Patterson, J. L., & Chilian, W. M. (1993) · Circulation · doi:10.1161/01.cir.87.4.1264 · Cited by 181
Calcium measurement in isolated arterioles during myogenic and agonist stimulation
Meininger, G. A., Zawieja, D. C., Falcone, J. C., Hill, M. A., & Davey, J. P. (1991) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.1991.261.3.h950 · Cited by 175
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Davis, M. J., & Hill, M. A. (1999) · Physiological Reviews · doi:10.1152/physrev.1999.79.2.387 · Cited by 992
Impaired conduction of vasodilation along arterioles in connexin40-deficient mice
de Wit, C., Roos, F., Bolz, S.-S., Kirchhoff, S., Krüger, O., Willecke, K., & Pohl, U. (2000) · Circulation Research · doi:10.1161/01.res.86.6.649 · Cited by 338
Endothelial cell expression of haemoglobin α regulates nitric oxide signalling
Straub, A. C., Lohman, A. W., Billaud, M., Johnstone, S. R., Dwyer, S. T., Lee, M. Y., Bortz, P. S., Best, A. K., Columbus, L., Gaston, B., et al. (2012) · Nature · doi:10.1038/nature11626 · Cited by 308
Emerson, G. G., & Segal, S. S. (2000) · Circulation Research · doi:10.1161/01.res.87.6.474 · Cited by 299
Davis, M. J., Earley, S., Li, Y.-S., & Chien, S. (2023) · Physiological Reviews · doi:10.1152/physrev.00053.2021 · Cited by 273
Metadata
- Authors
- Dora, K. A., Doyle, M. P., & Duling, B. R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1997-06-10
- Volume
- 94
- Issue
- 12
- Pages
- 6529-6534
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1994931711
- Cited by
- 332
- References
- 25
- FWCI
- 7.27
- Type
- journal article
- Language
- en
- Open access
- green
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- Dora, K. A., Doyle, M. P., & Duling, B. R. (1997). Elevation of intracellular calcium in smooth muscle causes endothelial cell generation of NO in arterioles. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.94.12.6529
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