Cited by 410Plant Stress Responses and ToleranceHeavy metals in environmentHeavy Metal Exposure and Toxicity

The DOI matches its Crossref record; 33 of the 34 references it cites were found in a registry or an index.

AtPCS1, a phytochelatin synthase from Arabidopsis : Isolation and in vitro reconstitution

Open access

Vatamaniuk, Olena K., Mari, Stéphane, Lu, Yu-Ping, Rea, Philip A.·1999·Proceedings of the National Academy of Sciences

DOI 10.1073/pnas.96.12.7110PMC PMC22073

Open this paper in CoChat — free account

Abstract

Phytochelatins, a class of posttranslationally synthesized peptides, play a pivotal role in heavy metal, primarily Cd2+, tolerance in plants and fungi by chelating these substances and decreasing their free concentrations. Derived from glutathione and related thiols by the action of gamma-glutamylcysteine dipeptidyl transpeptidases (phytochelatin synthases; EC 2.3.2.15), phytochelatins consist of repeating units of gamma-glutamylcysteine followed by a C-terminal Gly, Ser, or beta-Ala residue [poly-(gamma-Glu-Cys)n-Xaa]. Here we report the suppression cloning of a cDNA (AtPCS1) from Arabidopsis thaliana encoding a 55-kDa soluble protein that enhances heavy-metal tolerance and elicits Cd2+-activated phytochelatin accumulation when expressed in Saccharomyces cerevisiae. On the basis of these properties and the sufficiency of immunoaffinity-purified epitope-tagged AtPCS1 polypeptide for high rates of Cd2+-activated phytochelatin synthesis from glutathione in vitro, AtPCS1 is concluded to encode the enzyme phytochelatin synthase.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1073/pnas.96.12.7110

Cites

33 of 33 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302
An Algorithm for Least-Squares Estimation of Nonlinear Parameters
Marquardt, D. W. (1963) · Journal of the Society for Industrial and Applied Mathematics · doi:10.1137/0111030 · Cited by 30,728
Tissue sulfhydryl groups
Ellman, G. L. (1959) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(59)90090-6 · Cited by 26,824
CLUSTAL: a package for performing multiple sequence alignment on a microcomputer
Higgins, D. G., & Sharp, P. M. (1988) · Gene · doi:10.1016/0378-1119(88)90330-7 · Cited by 3,394
Fluorescamine: A Reagent for Assay of Amino Acids, Peptides, Proteins, and Primary Amines in the Picomole Range
Udenfriend, S., Stein, S., Böhlen, P., Dairman, W. M., Leimgruber, W., & Weigele, M. (1972) · Science · doi:10.1126/science.178.4063.871 · Cited by 2,639
METALLOTHIONEIN
Hamer, D. H. (1986) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.55.070186.004405 · Cited by 1,655
Metal ion-catalyzed oxidation of proteins: Biochemical mechanism and biological consequences
Stadtman, E. R. (1990) · Free Radical Biology and Medicine · doi:10.1016/0891-5849(90)90006-5 · Cited by 1,160
Heavy metal detoxification in higher plants - a review
Zenk, M. H. (1996) · Gene · doi:10.1016/s0378-1119(96)00422-2 · Cited by 1,003
Phytochelatins, the heavy-metal-binding peptides of plants, are synthesized from glutathione by a specific γ-glutamylcysteine dipeptidyl transpeptidase (phytochelatin synthase)
Grill, E., Löffler, S., Winnacker, E., & Zenk, M. H. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.18.6838 · Cited by 916
Tolerance to toxic metals by a gene family of phytochelatin synthases from plants and yeast
Clemens, S., Kim, E. J., Neumann, D., & Schroeder, J. I. (1999) · The EMBO Journal · doi:10.1093/emboj/18.12.3325 · Cited by 660

Show all 34 references

Research works printed but not linked: 5

D Voet, J G Voet Biochemistry (Wiley, 2nd Ed., New York, 1995).
J Zhou, P B Goldsbrough Plant Cell 6, 875–884 (1994).
M Minet, M Dufour, F Lacronte Plant J 2, 417–422 (1992). · DOI
Ha S.-B Smith A. P. Howden R. Dietrich W. M. Bugg S. O’Connell M. J. Goldsbrough P. B. & Cobbett C. S. (1999) Plant Cell in press.
Clemens S. Kim E. J. Neumann D. & Schroeder J. I. (1999) EMBO J. in press.

Sorted by citations

Items 201–300 of 406

Ectomycorrhizas: their role in forest ecosystems under the impact of acidifying pollutants
Brunner, I. L. (2001) · Perspectives in Plant Ecology Evolution and Systematics · doi:10.1078/1433-8319-00012 · Cited by 42
Phytoremediation to phytochelatin – plant trace metal homeostasis
Krämer, U. (2003) · New Phytologist · doi:10.1046/j.1469-8137.2003.00730.x · Cited by 42
Systematic engineering of phytochelatin synthesis and arsenic transport for enhanced arsenic accumulation in E. coli
Singh, S., Kang, S. H., Lee, W., Mulchandani, A., & Chen, W. (2009) · Biotechnology and Bioengineering · doi:10.1002/bit.22585 · Cited by 42
Phytoremediation Using Algae and Macrophytes: I
Mahmood, Q., Mirza, N., & Shaheen, S. (2015) · Springer eBooks · doi:10.1007/978-3-319-10969-5_22 · Cited by 41
Cloning and Characterization of a Phragmites australis Phytochelatin Synthase (PaPCS) and Achieving Cd Tolerance in Tall Fescue
Zhao, C., Xu, J., Li, Q., Li, S., Wang, P., & Xiang, F. (2014) · PLoS ONE · doi:10.1371/journal.pone.0103771 · Cited by 41
Biotechnological Approaches to Improve Phytoremediation Efficiency for Environment Contaminants
Singh, R. P., Dhania, G., Sharma, A., & Jaiwal, P. K. (2007) · Springer eBooks · doi:10.1007/978-3-540-34793-4_10 · Cited by 41
Phytochelatin and Oxidative Stress Under Heavy Metal Stress Tolerance in Plants
Liu, W., Zhang, X. Z., Liang, L., Chen, C., Wei, S., & Zhou, Q. (2015) · Springer eBooks · doi:10.1007/978-3-319-20421-5_8 · Cited by 43
Phytochelatins are synthesized by two vacuolar serine carboxypeptidases in Saccharomyces cerevisiae
Wünschmann, J., Beck, A., Meyer, L., Letzel, T., Grill, E., & Lendzian, K. J. (2007) · FEBS Letters · doi:10.1016/j.febslet.2007.03.039 · Cited by 39
Accumulation of metal‐binding peptides in fission yeast requires hmt2+
Weghe, J. G. V., & Ow, D. W. (2001) · Molecular Microbiology · doi:10.1046/j.1365-2958.2001.02624.x · Cited by 39
Determination and characterization of cysteine, glutathione and phytochelatins (PC2–6) in Lolium perenne L. exposed to Cd stress under ambient and elevated carbon dioxide using HPLC with fluorescence detection
Ju, X. H., Tang, S., Jia, Y., Guo, J., Ding, Y., Song, Z., & Zhao, Y. (2011) · Journal of Chromatography B · doi:10.1016/j.jchromb.2011.04.016 · Cited by 39
Characterization of the Phytochelatin Synthase of Schistosoma mansoni
Ray, D., & Williams, D. L. (2011) · PLoS neglected tropical diseases · doi:10.1371/journal.pntd.0001168 · Cited by 39
Tobacco phytochelatin synthase (NtPCS1) plays important roles in cadmium and arsenic tolerance and in early plant development in tobacco
Lee, B., & Hwang, S. (2015) · Plant Biotechnology Reports · doi:10.1007/s11816-015-0348-5 · Cited by 38
Ancestral function of the phytochelatin synthase C-terminal domain in inhibition of heavy metal-mediated enzyme overactivation
Li, M., Barbaro, E., Bellini, E., Saba, A., di Toppi, L. S., & Varotto, C. (2020) · Journal of Experimental Botany · doi:10.1093/jxb/eraa386 · Cited by 37
Adaptive Engineering of Phytochelatin-based Heavy Metal Tolerance
Cahoon, R. E., Lutke, W. K., Cameron, J. C., Chen, S., Lee, S. G., Rivard, R. S., Rea, P. A., & Jez, J. M. (2015) · Journal of Biological Chemistry · doi:10.1074/jbc.m115.652123 · Cited by 37
Self-citation
Metal Chelating Peptides and Proteins in Plants
di Toppi, L. S., Prasad, M. N. V., & Ottonello, S. (2002) · Springer eBooks · doi:10.1007/978-94-017-2660-3_3 · Cited by 37
Maize factors ZmUBP15, ZmUBP16 and ZmUBP19 play important roles for plants to tolerance the cadmium stress and salt stress
Kong, J., Jin, J., Dong, Q., Qiu, J., Li, Y., Yang, Y., Shi, Y., Si, W., Gu, L., Yang, F., et al. (2018) · Plant Science · doi:10.1016/j.plantsci.2018.11.014 · Cited by 36
Gene expression in cadmium-tolerant Datura innoxia: detection and characterization of cDNAs induced in response to Cd2+
Louie, M. C., Kondor, N., & DeWitt, J. G. (2003) · Plant Molecular Biology · doi:10.1023/a:1023926225931 · Cited by 36
Response to Heavy Metals in Plants: A Molecular Approach
di Toppi, L. S., Gremigni, P., Pawlik‐Skowrońska, B., Prasad, M. N. V., & Cobbett, C. S. (2003) · Springer eBooks · doi:10.1007/978-94-017-0255-3_6 · Cited by 34
Possible roles of phytochelatins and glutathione metabolism in cadmium tolerance in chickpea roots
Gupta, D. K., Tohoyama, H., Joho, M., & Inouhe, M. (2002) · Journal of Plant Research · doi:10.1007/s10265-002-0055-5 · Cited by 34
Role of Phytochelatins in Phytoremediation of Heavy Metals
Grill, E., Mishra, S., Srivastava, S., & Tripathi, R. D. (2007) · Springer eBooks · doi:10.1007/978-3-540-34793-4_5 · Cited by 34
Cadmium sensitivity, uptake, subcellular distribution and thiol induction in a marine diatom: Exposure to cadmium
Wang, M., & Wang, W. (2010) · Aquatic Toxicology · doi:10.1016/j.aquatox.2010.11.014 · Cited by 33
Detoxification of Heavy Metals: State of Art
Agrawal, J., Sherameti, I., & Varma, A. K. (2011) · Soil biology · doi:10.1007/978-3-642-21408-0_1 · Cited by 33
Transgenic Plants for Enhanced Phytoremediation – Physiological Studies
Mello-Farias, P., Chaves, A. L. S., & Lencina, C. L. (2011) · InTech eBooks · doi:10.5772/24355 · Cited by 33
New insights into the regulation of phytochelatin biosynthesis in A. thaliana cells from metabolite profiling analyses
Ducruix, C., Junot, C., Fiévet, J., Villiers, F., Ezan, É., & Bourguignon, J. (2006) · Biochimie · doi:10.1016/j.biochi.2006.08.005 · Cited by 32
Expression of Arabidopsis Phytochelatin Synthase 2 Is Too Low to Complement an AtPCS1-defective Cad1-3 Mutant
Lee, S., & Kang, B. S. (2005) · Molecules and Cells · doi:10.1016/s1016-8478(23)13140-2 · Cited by 32
Gene Functional Analysis Using Protoplast Transient Assays
Jung, H., Yan, J., Zhai, Z., & Vatamaniuk, O. K. (2015) · Methods in molecular biology · doi:10.1007/978-1-4939-2444-8_22 · Cited by 31
Self-citation
Functional characterisation of two phytochelatin synthases in rice (Oryza sativa cv. Milyang 117) that respond to cadmium stress
Park, H. C., Hwang, J.-W., Jiang, Y., Kim, Y. J., Kim, S. H., Nguyen, X. C., Kim, C. Y., & Chung, W. S. (2019) · Plant Biology · doi:10.1111/plb.12991 · Cited by 31
Evolution and functional differentiation of recently diverged phytochelatin synthase genes from Arundo donax L.
Li, M., Stragliati, L., Bellini, E., Ricci, A., Saba, A., di Toppi, L. S., & Varotto, C. (2019) · Journal of Experimental Botany · doi:10.1093/jxb/erz266 · Cited by 31
Phytochelatin is not a primary factor in determining copper tolerance
Lee, S., & Kang, B. S. (2005) · Journal of Plant Biology · doi:10.1007/bf03030562 · Cited by 30
Relief of Arsenate Toxicity by Cd-Stimulated Phytochelatin Synthesis in the Green Alga Chlamydomonas reinhardtii
Kobayashi, I., Fujiwara, S., Saegusa, H., Inouhe, M., Matsumoto, H., & Tsuzuki, M. (2005) · Marine Biotechnology · doi:10.1007/s10126-005-5092-3 · Cited by 30
Oxidative stress responses induced by uranium exposure at low pH in leaves of Arabidopsis thaliana plants
Saenen, E., Horemans, N., Vanhoudt, N., Vandenhove, H., Biermans, G., Hees, M. V., Wannijn, J., Vangronsveld, J., & Cuypers, A. (2015) · Journal of Environmental Radioactivity · doi:10.1016/j.jenvrad.2015.07.021 · Cited by 30
Enhanced tolerance to and accumulation of mercury, but not arsenic, in plants overexpressing two enzymes required for thiol peptide synthesis
Li, Y., Heaton, A. C. P., Carreira, L. H., & Meagher, R. B. (2006) · Physiologia Plantarum · doi:10.1111/j.1399-3054.2006.00732.x · Cited by 29
Identification of an arsenic tolerant double mutant with a thiol‐mediated component and increased arsenic tolerance in phyA mutants
Sung, D., Lee, D., Harris, H. H., Raab, A., Feldmann, J., Meharg, A. A., Kumabe, B., Komives, E. A., & Schroeder, J. I. (2007) · The Plant Journal · doi:10.1111/j.1365-313x.2006.03018.x · Cited by 29
The First Cadmium-Specific Enzyme
Strasdeit, H. (2001) · Angewandte Chemie International Edition · doi:10.1002/1521-3773(20010216)40:4 · Cited by 29
AtTIP2;2 facilitates resistance to zinc toxicity via promoting zinc immobilization in the root and limiting root-to-shoot zinc translocation in Arabidopsis thaliana
Wang, Y., Kang, Y., Yu, W., Lyi, S. M., Choi, H. W., Xiao, E., Li, L., Klessig, D. F., & Liu, J. (2022) · Ecotoxicology and Environmental Safety · doi:10.1016/j.ecoenv.2022.113333 · Cited by 28
Pseudomonas syringae enhances herbivory by suppressing the reactive oxygen burst in Arabidopsis
Groen, S. C., Humphrey, P. T., Chevasco, D., Ausubel, F. M., Pierce, N. E., & Whiteman, N. K. (2015) · Journal of Insect Physiology · doi:10.1016/j.jinsphys.2015.07.011 · Cited by 28
Overexpression of phytochelatin synthase AtPCS2 enhances salt tolerance in Arabidopsis thaliana
Kim, Y., Kang, H., & Ahn, S. (2019) · Journal of Plant Physiology · doi:10.1016/j.jplph.2019.153011 · Cited by 28
Leaf-targeted phytochelatin synthase in Arabidopsis thaliana
Peterson, A. G., & Oliver, D. J. (2006) · Plant Physiology and Biochemistry · doi:10.1016/j.plaphy.2006.10.021 · Cited by 27
Role of Phytochelatins in Phytoremediation of Heavy Metals Contaminated Soils
Srivastava, N. (2016) · Springer eBooks · doi:10.1007/978-3-319-40148-5_13 · Cited by 26
Phytochelatin synthase de-regulation in Marchantia polymorpha indicates cadmium detoxification as its primary ancestral function in land plants and provides a novel visual bioindicator for detection of this metal
Li, M., Leso, M., Buti, M., Bellini, E., Bertoldi, D., Saba, A., Larcher, R., di Toppi, L. S., & Varotto, C. (2022) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2022.129844 · Cited by 26
Metallothioneins and Phytochelatins: Role and Perspectives in Heavy Metal(loid)s Stress Tolerance in Crop Plants
Shukla, D., Trivedi, P. K., Nath, P., & Tuteja, N. K. (2016) · Wiley eBooks · doi:10.1002/9783527694570.ch12 · Cited by 26
Metabolomic investigation of the response of the model plant Arabidopsis thaliana to cadmium exposure: Evaluation of data pretreatment methods for further statistical analyses
Ducruix, C., Vailhen, D., Werner, E., Fiévet, J., Bourguignon, J., Tabet, J., Ezan, É., & Junot, C. (2007) · Chemometrics and Intelligent Laboratory Systems · doi:10.1016/j.chemolab.2007.08.002 · Cited by 25
Arsenite Elicits Anomalous Sulfur Starvation Responses in Barley
Reid, R. J., Gridley, K., Kawamata, Y., & Zhu, Y. (2013) · PLANT PHYSIOLOGY · doi:10.1104/pp.113.216937 · Cited by 25
BjHO-1 is involved in the detoxification of heavy metal in India mustard (Brassica juncea)
Li, H., Jiang, M., Li, L., Nie, L., & Yang, Z. M. (2012) · BioMetals · doi:10.1007/s10534-012-9588-9 · Cited by 23
Metal Ion-Binding Properties of Phytochelatins and Related Ligands
Devez, A., Achterberg, E. P., & Gledhill, M. (2009) · Royal Society of Chemistry eBooks · doi:10.1039/9781847559531-00441 · Cited by 25
The Role of Glutathione in Plant Reaction and Adaptation to Excess Metals
Rauser, W. E. (2001) · Plant ecophysiology · doi:10.1007/0-306-47644-4_6 · Cited by 23
Overexpression of phytochelatin synthase (pcs) enhances abiotic stress tolerance by altering the proteome of transformed Anabaena sp. PCC 7120
Chaurasia, N., Mishra, Y., Chatterjee, A., Rai, R., Yadav, S., & Rai, L. C. (2016) · PROTOPLASMA · doi:10.1007/s00709-016-1059-7 · Cited by 23
Sulfur Assimilation and Cadmium Tolerance in Plants
Anjum, N. A., Umar, S., Singh, S., Nazar, R., & Khan, N. A. (2008) · Springer eBooks · doi:10.1007/978-3-540-76326-0_13 · Cited by 24
Cadmium response of the hairy root culture of the endangered species Adenophora lobophylla
Wu, S., Zu, Y., & Wu, M. C. S. (2001) · Plant Science · doi:10.1016/s0168-9452(00)00429-5 · Cited by 22
Phytoremediation of Trace Element–Contaminated Environments and the Potential of Endophytic Bacteria for Improving this Process
Cherian, S., Weyens, N., Lindberg, S. M., & Vangronsveld, J. (2012) · Critical Reviews in Environmental Science and Technology · doi:10.1080/10643389.2011.574106 · Cited by 22
Characterization of differentially expressed genes to Cu stress in Brassica nigra by Arabidopsis genome arrays
Keskin, B. C., Yıldızhan, Y., Yüksel, B., Dalyan, E., & Memon, A. R. (2018) · Environmental Science and Pollution Research · doi:10.1007/s11356-018-3577-7 · Cited by 22
Oppia nitens C.L. Koch, 1836 (Acari: Oribatida): Current Status of Its Bionomics and Relevance as a Model Invertebrate in Soil Ecotoxicology
Fajana, H. O., Gainer, A., Jegede, O., Awuah, K. F., Princz, J., Owojori, O. J., & Siciliano, S. D. (2019) · Environmental Toxicology and Chemistry · doi:10.1002/etc.4574 · Cited by 22
Recent Trends and Approaches in Phytoremediation
Malik, B., Pirzadah, T. B., Tahir, I., Dar, T. U. H., & Rehman, R. U. (2014) · Elsevier eBooks · doi:10.1016/b978-0-12-799937-1.00006-1 · Cited by 22
Iris lactea var. chinensis (Fisch.) cysteine-rich gene llCDT1 enhances cadmium tolerance in yeast cells and Arabidopsis thaliana
Gu, C., Liu, L., Song, A., Liu, Z., Zhang, Y., & Huang, S. (2018) · Ecotoxicology and Environmental Safety · doi:10.1016/j.ecoenv.2018.03.059 · Cited by 21
Overexpression of the maize phytochelatin synthase gene (ZmPCS1) enhances Cd tolerance in plants
Jin, D., Zhang, Q., Liu, Y., Liang, M., Li, A., & Wu, J. (2022) · Acta Physiologiae Plantarum · doi:10.1007/s11738-022-03451-1 · Cited by 21
Bioremediation Technologies for the Removal of Pollutants
Dhir, B. (2017) · Advances in Environmental Biotechnology · doi:10.1007/978-981-10-4041-2_5 · Cited by 20
Histidine-Rich Defensins from the Solanaceae and Brasicaceae Are Antifungal and Metal Binding Proteins
Bleackley, M. R., Vasa, S., Harvey, P. J., Shafee, T. M. A., Kerenga, B. K., da Costa, T. P. S., Craik, D. J., Lowe, R. G. T., & Anderson, M. A. (2020) · Journal of Fungi · doi:10.3390/jof6030145 · Cited by 20
Characterization and Expression of a Phytochelatin Synthase Gene in Birch-leaf Pear (Pyrus betulaefolia Bunge)
Chang, Y. H., Li, H., Cong, Y., Lin, J., & SHENG, B.-L. (2012) · Plant Molecular Biology Reporter · doi:10.1007/s11105-012-0447-1 · Cited by 20
The phytochelatin synthase gene in date palm ( Phoenix dactylifera L.): Phylogeny, evolution and expression
Chaâbene, Z., Rekik, I., Kriaâ, W., Grubb, C. D., Khemakhem, B., Vandenbulcke, F., Mejdoub, H., & Elleuch, A. (2017) · Ecotoxicology and Environmental Safety · doi:10.1016/j.ecoenv.2017.02.020 · Cited by 20
Phytochelatin Synthase in Heavy Metal Detoxification and Xenobiotic Metabolism
Chia, J. (2021) · IntechOpen eBooks · doi:10.5772/intechopen.99077 · Cited by 19
Co‐expression of Arabidopsis thaliana phytochelatin synthase and Treponema denticola cysteine desulfhydrase for enhanced arsenic accumulation
Tsai, S., Singh, S., DaSilva, N. A., & Chen, W. (2011) · Biotechnology and Bioengineering · doi:10.1002/bit.23325 · Cited by 19
Green Syntheis Of Silver Nanoparticles And Its Activity On SiHa Cervical Cancer Cell Line
Jha, A. K., & Prasad, K. (2014) · Advanced Materials Letters · doi:10.5185/amlett.2014.4563 · Cited by 19
Potential Implications of Interactions between Fe and S on Cereal Fe Biofortification
Kawakami, Y., & Bhullar, N. K. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21082827 · Cited by 19
Metal Hyperaccumulation and Tolerance in Alyssum, Arabidopsis and Thlaspi: An Overview
Gill, S. S., Anjum, N. A., Ahmad, I., Thangavel, P., Sridevi, G., Pacheco, M., Duarte, A. C., Umar, S., Khan, N. A., & Pereira, E. (2012) · Environmental pollution · doi:10.1007/978-94-007-3913-0_4 · Cited by 19
Expression of tobacco cDNA encoding phytochelatin synthase promotes tolerance to and accumulation of Cd and As inSaccharomyces cerevisiae
Kim, Y. J., Chang, K. S., Lee, M. R., Kim, J., Lee, C. E., Jeon, Y. J., Choi, J. S., Shin, H. S., & Hwang, S. (2005) · Journal of Plant Biology · doi:10.1007/bf03030586 · Cited by 19
Identifying sugarcane expressed sequences associated with nutrient transporters and peptide metal chelators
Figueira, A., Kido, É. A., & Almeida, R. S. (2001) · Genetics and Molecular Biology · doi:10.1590/s1415-47572001000100028 · Cited by 19
Rapid Isolation of Monoclonal Antibodies. Monitoring Enzymes in the Phytochelatin Synthesis Pathway
Li, Y., Kandasamy, M. K., & Meagher, R. B. (2001) · PLANT PHYSIOLOGY · doi:10.1104/pp.010413 · Cited by 19
Differences in the Binding Modes of Phytochelatin to Cadmium(II) and Zinc(II) Ions
Kobayashi, R., & Yoshimura, E. (2006) · Biological Trace Element Research · doi:10.1385/bter:114:1:313 · Cited by 18
Homologous cloning, characterization and expression of a new halophyte phytochelatin synthase gene in Suaeda salsa
Cong, M., Zhao, J., Lü, J., Ren, Z., & Wu, H. (2016) · Chinese Journal of Oceanology and Limnology · doi:10.1007/s00343-016-4382-0 · Cited by 18
Glutathione regulates transcriptional activation of iron transporters via S ‐nitrosylation of bHLH factors to modulate subcellular iron homoeostasis
Shee, R., Ghosh, S., Khan, P., Sahid, S., Roy, C., Shee, D., Paul, S., & Datta, R. (2022) · Plant Cell & Environment · doi:10.1111/pce.14331 · Cited by 18
Breeding Oil Crops for Sustainable Production: Heavy Metal Tolerance
Farooq, M. A., Ali, B., Gill, R. A., Islam, F., Cui, P., & Zhou, W. (2015) · Elsevier eBooks · doi:10.1016/b978-0-12-801309-0.00002-1 · Cited by 18
Expression of OsHARBI1-1 enhances the tolerance of Arabidopsis thaliana to cadmium
Jiang, N., Shi, Y., Li, M., Du, Z., Chen, J., Jiang, W., Huang, Y., Zhong, M., Yang, J., Hu, B., et al. (2023) · BMC Plant Biology · doi:10.1186/s12870-023-04540-0 · Cited by 17
Improving biosynthesis of Au Pd core-shell nanoparticles through Escherichia coli with the assistance of phytochelatin for catalytic enhanced chemiluminescence and benzyl alcohol oxidation
Zhang, D., Tang, D., Yamamoto, T., Kato, Y., Horiuchi, S., Ogawa, S., Yoshimura, E., & Suzuki, M. (2019) · Journal of Inorganic Biochemistry · doi:10.1016/j.jinorgbio.2019.110795 · Cited by 17
Deletion of Phytochelatin Synthase Modulates the Metal Accumulation Pattern of Cadmium Exposed C. elegans
Essig, Y. J., Webb, S. M., & Stürzenbaum, S. R. (2016) · International Journal of Molecular Sciences · doi:10.3390/ijms17020257 · Cited by 17
Characterization of the Sesbania rostrata Phytochelatin Synthase Gene: Alternative Splicing and Function of Four Isoforms
Li, A., Yu, B.-Y., Chen, F., Gan, H., Yuan, J., Qiu, R., Huang, J.-C., Yang, Z., & Xu, Z. (2009) · International Journal of Molecular Sciences · doi:10.3390/ijms10083269 · Cited by 17
Metal immobilization: where and how
Mari, S., & Lebrun, M. (2005) · Topics in Current Genetics · doi:10.1007/4735_103 · Cited by 15
Self-citation
Potential Hazardous Elements Fluxes from Soil to Plants and the Food Chain
Abreu, M. M., Bech, J., Carvalho, L. C., & Santos, E. S. (2014) · Springer eBooks · doi:10.1007/978-94-017-8965-3_8 · Cited by 15
The N-Terminal Extension Domain of the C. elegans Half-Molecule ABC Transporter, HMT-1, Is Required for Protein-Protein Interactions and Function
Kim, S., Selote, D. S., & Vatamaniuk, O. K. (2010) · PLoS ONE · doi:10.1371/journal.pone.0012938 · Cited by 15
Self-citation
Genome-wide identification of PHYTOCHELATIN and PHYTOCH_SYNTH domain-containing phytochelatin family from rice
Shen, G., Zhu, C., & Du, Q. (2010) · Electronic journal of biology · Cited by 15
Impact of proteomics on anti-Mycobacterium tuberculosis (MTB) vaccine development.
Jagusztyn-Krynicka, E. K., Roszczenko, P., & Grabowska, A. D. (2009) · PubMed · Cited by 15
Metallothioneins and Phytochelatins: Molecular Aspects
Cobbett, C. S. (2003) · Springer eBooks · doi:10.1007/978-94-017-0289-8_10 · Cited by 15
Cadmium Metal Detoxification and Hyperaccumulators
Shah, K. (2011) · Soil biology · doi:10.1007/978-3-642-21408-0_10 · Cited by 14
Cloning and characterization of phytochelatin synthase from a Nickel hyperaccumulatorThlaspi japonicumand its expression in yeast
Mizuno, T., Sonoda, T., Horie, K., Senoo, K., Tanaka, A., Mizuno, N., & Obata, H. (2003) · Soil Science & Plant Nutrition · doi:10.1080/00380768.2003.10410009 · Cited by 14
Enhancing the tolerance of zebrafish (Danio rerio) to heavy metal toxicity by the expression of plant phytochelatin synthase
Konishi, T., Matsumoto, S., Tsuruwaka, Y., Shiraki, K., Hirata, K., Tamaru, Y., & Takagi, M. (2006) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2005.12.013 · Cited by 14
Heterologous expression of cyanobacterial PCS confers augmented arsenic and cadmium stress tolerance and higher artemisinin in Artemisia annua hairy roots
Pandey, N., Rai, K. K., Rai, S. K., & Rai, S. P. (2021) · Plant Biotechnology Reports · doi:10.1007/s11816-021-00682-5 · Cited by 14
Das erste cadmiumspezifische Enzym
Strasdeit, H. (2001) · Angewandte Chemie · doi:10.1002/1521-3757(20010216)113:4 · Cited by 14
Isolation of Three Metallothionein Genes and Their Roles in Mediating Cadmium Resistance
Zhang, P., Zhang, X.-J., Tang, T.-W., Hu, H.-L., Bai, N.-N., Zhang, D., Meng, S., & Peng, J. (2022) · Agronomy · doi:10.3390/agronomy12122971 · Cited by 13
Characterization of a Nicotiana tabacum phytochelatin synthase 1 and its response to cadmium stress
Wu, C., Zhang, J., Chen, M., Liu, J., & Tang, Y. (2024) · Frontiers in Plant Science · doi:10.3389/fpls.2024.1418762 · Cited by 14
Effects of SpGSH1 and SpPCS1 overexpression or co-overexpression on cadmium accumulation in yeast and Spirodela polyrhiza
Chen, Y., Yang, J., Zhao, X., Sun, Z., Li, G., Hussain, S., Li, X., Zhang, L., Wang, Z., Gong, H., et al. (2024) · Plant Physiology and Biochemistry · doi:10.1016/j.plaphy.2024.109097 · Cited by 13
Heavy Metal Resistance and Phytoremediation with Transgenic Trees
Peuke, A. D., & Rennenberg, H. (2006) · Springer eBooks · doi:10.1007/3-540-32199-3_7 · Cited by 13
Dequenching of Cu(I)−Bathocuproinedisulfonate Complexes for High-Performance Liquid Chromatographic Determination of Phytochelatins, Heavy-Metal-Binding Peptides Produced by the Primitive Red Alga Cyanidioschyzon merolae
Shirabe, T., Ito, K., & Yoshimura, E. (2008) · Analytical Chemistry · doi:10.1021/ac801741b · Cited by 13
Transcriptional landscape illustrates the diversified adaptation of medicinal plants to multifactorial stress combinations linked with high altitude
Kumari, M., Kumar, P. P., Saini, V., Joshi, R., Shankar, P. R., & Kumar, R. (2025) · Planta · doi:10.1007/s00425-025-04686-1 · Cited by 12
In silico prediction, phylogenetic and bioinformatic analysis of SoPCS gene, survey of its protein characterization and gene expression in response to cadmium in Saccharum officinarum
Kolahi, M., Yazdi, M., Goldson-Barnaby, A., & Tabandeh, M. R. (2018) · Ecotoxicology and Environmental Safety · doi:10.1016/j.ecoenv.2018.07.032 · Cited by 12
Detection of Phytochelatin in the Xylem Sap of Brassica napus
Saathoff, A. J., Ahner, B. A., Spanswick, R. M., & Walker, L. P. (2010) · Environmental Engineering Science · doi:10.1089/ees.2010.0183 · Cited by 12
General Roles of Phytochelatins and Other Peptides in Plant Defense Mechanisms Against Oxidative Stress/Primary and Secondary Damages Induced by Heavy Metals
Inouhe, M., Sakuma, Y., Chatterjee, S., Datta, S., Jagetiya, B., Voronina, A. V., Walther, C., & Gupta, D. K. (2015) · Springer eBooks · doi:10.1007/978-3-319-20421-5_9 · Cited by 12
Combating plant diseases through transition metal allocation
De, A., Hoang, C. V., Escudero, V., Armas, A. M., Echávarri‐Erasun, C., González‐Guerrero, M., & Jordá, L. (2024) · New Phytologist · doi:10.1111/nph.20366 · Cited by 12
Functional characterization of Helianthus annuus phytochelatin synthase (HaPCS): Gene expression and protein profiles of HaPCS responding to arsenic and evaluation of arsenic accumulation in engineered bacteria expressing HaPCS
Azizi, N., & Shahpiri, A. (2021) · Environmental and Experimental Botany · doi:10.1016/j.envexpbot.2021.104470 · Cited by 11
Importance of sulfur and its roles in Plants physiology: A Review
(2020) · Current Science International · doi:10.36632/csi/2020.9.2.18 · Cited by 11
Responses and Adaptations of Plants to Metal Stress
Salt, D. E. (2001) · Springer eBooks · doi:10.1007/978-94-015-9783-8_8 · Cited by 11
Genetic Engineering for Metal and Metalloid Detoxification
Weerakoon, S. R. (2019) · Elsevier eBooks · doi:10.1016/b978-0-12-814389-6.00002-x · Cited by 11

Authors: Vatamaniuk, O. K., Mari, S., Lu, Y.-P., & Rea, P. A.
Affiliations: University of Pennsylvania (US)
ORCID: Vatamaniuk, Olena K.
Mari, Stéphane
Venue: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 1999-06-08
Volume: 96
Issue: 12
Pages: 7110-7115
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.96.12.7110
PMC: PMC22073
OpenAlex: W2096818569
Cited by: 410
References: 34
Citations by year: 2026: 6 · 2025: 4 · 2024: 6 · 2023: 8 · 2022: 13 · 2021: 9 · 2020: 11 · 2019: 11 · 2018: 11 · 2017: 8 · 2016: 10 · 2015: 22 · 2014: 8 · 2013: 12 · 2012: 19
FWCI: 29.02
Type: journal article
Language: en
Open access: green
europepmc.org
Locations: Proceedings of the National Academy of Sciences (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
Physical Sciences › Environmental Science › Pollution › Heavy metals in environment
Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Heavy Metal Exposure and Toxicity
Keywords: phytochelatin synthase, phytochelatins, cadmium tolerance, heavy metal tolerance, glutathione, Arabidopsis thaliana, Saccharomyces cerevisiae expression, metal chelation
MeSH terms: Amino Acid Sequence, Molecular Sequence Data, Plant Proteins, Saccharomyces cerevisiae, Sequence Alignment, Arabidopsis, Aminoacyltransferases

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

AtPCS1, a phytochelatin synthase from Arabidopsis : Isolation and in vitro reconstitution

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Self-check

Citations

Cites

Cited by

Related

Metadata

Record

About these checks