Cited by 410Plant Stress Responses and ToleranceHeavy metals in environmentHeavy Metal Exposure and Toxicity

The DOI matches its Crossref record; 33 of the 34 references it cites were found in a registry or an index.

AtPCS1, a phytochelatin synthase from Arabidopsis : Isolation and in vitro reconstitution

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Vatamaniuk, Olena K., Mari, Stéphane, Lu, Yu-Ping, Rea, Philip A.·1999·Proceedings of the National Academy of Sciences

Abstract

Phytochelatins, a class of posttranslationally synthesized peptides, play a pivotal role in heavy metal, primarily Cd2+, tolerance in plants and fungi by chelating these substances and decreasing their free concentrations. Derived from glutathione and related thiols by the action of gamma-glutamylcysteine dipeptidyl transpeptidases (phytochelatin synthases; EC 2.3.2.15), phytochelatins consist of repeating units of gamma-glutamylcysteine followed by a C-terminal Gly, Ser, or beta-Ala residue [poly-(gamma-Glu-Cys)n-Xaa]. Here we report the suppression cloning of a cDNA (AtPCS1) from Arabidopsis thaliana encoding a 55-kDa soluble protein that enhances heavy-metal tolerance and elicits Cd2+-activated phytochelatin accumulation when expressed in Saccharomyces cerevisiae. On the basis of these properties and the sufficiency of immunoaffinity-purified epitope-tagged AtPCS1 polypeptide for high rates of Cd2+-activated phytochelatin synthesis from glutathione in vitro, AtPCS1 is concluded to encode the enzyme phytochelatin synthase.

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  1. D Voet, J G Voet Biochemistry (Wiley, 2nd Ed., New York, 1995).
  2. J Zhou, P B Goldsbrough Plant Cell 6, 875–884 (1994).
  3. M Minet, M Dufour, F Lacronte Plant J 2, 417–422 (1992). · DOI
  4. Ha S.-B Smith A. P. Howden R. Dietrich W. M. Bugg S. O’Connell M. J. Goldsbrough P. B. & Cobbett C. S. (1999) Plant Cell in press.
  5. Clemens S. Kim E. J. Neumann D. & Schroeder J. I. (1999) EMBO J. in press.

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Metadata

Authors
Vatamaniuk, O. K., Mari, S., Lu, Y.-P., & Rea, P. A.
Affiliations
University of Pennsylvania (US)
Venue
Proceedings of the National Academy of Sciences
Published
1999-06-08
Volume
96
Issue
12
Pages
7110-7115
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2096818569
Cited by
410
References
34
Citations by year
2026: 6 · 2025: 4 · 2024: 6 · 2023: 8 · 2022: 13 · 2021: 9 · 2020: 11 · 2019: 11 · 2018: 11 · 2017: 8 · 2016: 10 · 2015: 22 · 2014: 8 · 2013: 12 · 2012: 19
FWCI
29.02
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
  • Physical Sciences › Environmental Science › Pollution › Heavy metals in environment
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Heavy Metal Exposure and Toxicity
Keywords
phytochelatin synthase, phytochelatins, cadmium tolerance, heavy metal tolerance, glutathione, Arabidopsis thaliana, Saccharomyces cerevisiae expression, metal chelation
MeSH terms
Amino Acid Sequence, Molecular Sequence Data, Plant Proteins, Saccharomyces cerevisiae, Sequence Alignment, Arabidopsis, Aminoacyltransferases

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