Cited by 410Plant Stress Responses and ToleranceHeavy metals in environmentHeavy Metal Exposure and Toxicity

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AtPCS1, a phytochelatin synthase from Arabidopsis : Isolation and in vitro reconstitution

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Vatamaniuk, Olena K., Mari, Stéphane, Lu, Yu-Ping, Rea, Philip A.·1999·Proceedings of the National Academy of Sciences

DOI 10.1073/pnas.96.12.7110PMC PMC22073

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Abstract

Phytochelatins, a class of posttranslationally synthesized peptides, play a pivotal role in heavy metal, primarily Cd2+, tolerance in plants and fungi by chelating these substances and decreasing their free concentrations. Derived from glutathione and related thiols by the action of gamma-glutamylcysteine dipeptidyl transpeptidases (phytochelatin synthases; EC 2.3.2.15), phytochelatins consist of repeating units of gamma-glutamylcysteine followed by a C-terminal Gly, Ser, or beta-Ala residue [poly-(gamma-Glu-Cys)n-Xaa]. Here we report the suppression cloning of a cDNA (AtPCS1) from Arabidopsis thaliana encoding a 55-kDa soluble protein that enhances heavy-metal tolerance and elicits Cd2+-activated phytochelatin accumulation when expressed in Saccharomyces cerevisiae. On the basis of these properties and the sufficiency of immunoaffinity-purified epitope-tagged AtPCS1 polypeptide for high rates of Cd2+-activated phytochelatin synthesis from glutathione in vitro, AtPCS1 is concluded to encode the enzyme phytochelatin synthase.

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Authors: Vatamaniuk, O. K., Mari, S., Lu, Y.-P., & Rea, P. A.
Affiliations: University of Pennsylvania (US)
ORCID: Vatamaniuk, Olena K.
Mari, Stéphane
Venue: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 1999-06-08
Volume: 96
Issue: 12
Pages: 7110-7115
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.96.12.7110
PMC: PMC22073
OpenAlex: W2096818569
Cited by: 410
References: 34
Citations by year: 2026: 6 · 2025: 4 · 2024: 6 · 2023: 8 · 2022: 13 · 2021: 9 · 2020: 11 · 2019: 11 · 2018: 11 · 2017: 8 · 2016: 10 · 2015: 22 · 2014: 8 · 2013: 12 · 2012: 19
FWCI: 29.02
Type: journal article
Language: en
Open access: green
europepmc.org
Locations: Proceedings of the National Academy of Sciences (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
Physical Sciences › Environmental Science › Pollution › Heavy metals in environment
Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Heavy Metal Exposure and Toxicity
Keywords: phytochelatin synthase, phytochelatins, cadmium tolerance, heavy metal tolerance, glutathione, Arabidopsis thaliana, Saccharomyces cerevisiae expression, metal chelation
MeSH terms: Amino Acid Sequence, Molecular Sequence Data, Plant Proteins, Saccharomyces cerevisiae, Sequence Alignment, Arabidopsis, Aminoacyltransferases

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AtPCS1, a phytochelatin synthase from Arabidopsis : Isolation and in vitro reconstitution

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