The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
The kinetic mechanism of myosin V
Open accessAbstract
Myosin V is an unconventional myosin proposed to be processive on actin filaments, analogous to kinesin on a microtubule [Mehta, A. D., et al . (1999) Nature (London) 400, 590–593]. To ascertain the unique properties of myosin V that permit processivity, we undertook a detailed kinetic analysis of the myosin V motor. We expressed a truncated, single-headed myosin V construct that bound a single light chain to study its innate kinetics, free from constraints imposed by other regions of the molecule. The data demonstrate that unlike any previously characterized myosin a single-headed myosin V spends most of its kinetic cycle (>70%) strongly bound to actin in the presence of ATP. This kinetic tuning is accomplished by increasing several of the rates preceding strong binding to actin and concomitantly prolonging the duration of the strongly bound state by slowing the rate of ADP release. The net result is a myosin unlike any previously characterized, in that ADP release is the rate-limiting step for the actin-activated ATPase cycle. Thus, because of a number of kinetic adaptations, myosin V is tuned for processive movement on actin and will be capable of transporting cargo at lower motor densities than any other characterized myosin.
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Cites
Direct observation of single kinesin molecules moving along microtubules
Vale, R. D., Funatsu, T., Pierce, D. W., Romberg, L., Harada, Y., & Yanagida, T. (1996) · Nature · doi:10.1038/380451a0 · Cited by 711
Cited by
The Way Things Move: Looking Under the Hood of Molecular Motor Proteins
Vale, R. D., & Milligan, R. A. (2000) · Science · doi:10.1126/science.288.5463.88 · Cited by 1,503
Myosin-V stepping kinetics: A molecular model for processivity
Rief, M., Rock, R. S., Mehta, A. D., Mooseker, M. S., Cheney, R. E., & Spudich, J. A. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.17.9482 · Cited by 402
The gated gait of the processive molecular motor, myosin V
Veigel, C., Wang, F., Bartoo, M. L., Sellers, J. R., & Molloy, J. E. (2002) · Nature Cell Biology · doi:10.1038/ncb732 · Cited by 371
Kinetic Mechanism and Regulation of Myosin VI
De La Cruz, E. M., Ostap, E. M., & Sweeney, H. L. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m104136200 · Cited by 234
Self-citationConformational changes during kinesin motility
Schief, W. R., & Howard, J. (2001) · Current Opinion in Cell Biology · doi:10.1016/s0955-0674(00)00169-1 · Cited by 131
Functional diversity among a family of human skeletal muscle myosin motors
Resnicow, D. I., Deacon, J. C., Warrick, H. M., Spudich, J. A., & Leinwand, L. A. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0913527107 · Cited by 116
Myosin-V makes two brownian 90° rotations per 36-nm step
Komori, Y., Iwane, A. H., & Yanagida, T. (2007) · Nature Structural & Molecular Biology · doi:10.1038/nsmb1298 · Cited by 31
Porter, J. R., Meller, A., Zimmerman, M. I., Greenberg, M. J., & Bowman, G. R. (2020) · eLife · doi:10.7554/elife.55132 · Cited by 31
Pires, I., Hung, Y.-F., Bergmann, U., Molloy, J. E., & Kursula, I. (2022) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2022.102634 · Cited by 1
Porter, J. R., Meller, A., Zimmerman, M. I., Greenberg, M. J., & Bowman, G. R. (2019) · doi:10.1101/2019.12.16.878264
Metadata
- Authors
- De La Cruz, E. M., Wells, A. L., Rosenfeld, S. S., Ostap, E. M., & Sweeney, H. L.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1999-11-23
- Volume
- 96
- Issue
- 24
- Pages
- 13726-13731
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2001758775
- Cited by
- 418
- References
- 1
- FWCI
- 3.89
- Type
- journal article
- Language
- en
- Open access
- green
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