The Way Things Move: Looking Under the Hood of Molecular Motor Proteins
Abstract
The microtubule-based kinesin motors and actin-based myosin motors generate motions associated with intracellular trafficking, cell division, and muscle contraction. Early studies suggested that these molecular motors work by very different mechanisms. Recently, however, it has become clear that kinesin and myosin share a common core structure and convert energy from adenosine triphosphate into protein motion using a similar conformational change strategy. Many different types of mechanical amplifiers have evolved that operate in conjunction with the conserved core. This modular design has given rise to a remarkable diversity of kinesin and myosin motors whose motile properties are optimized for performing distinct biological functions.
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Members of the kinesin family of molecular motors are capable of taking over 100 steps on their microtubule track without dissociating.
“Members of the kinesin family of molecular motors are capable of taking over 100 steps on their microtubule track without dissociating”
From Stepping and Stretching (2003) · cites this paper as [3]
Cites
Direct observation of kinesin stepping by optical trapping interferometry
Svoboda, K., Schmidt, C. F., Schnapp, B. J., & Block, S. M. (1993) · Nature · doi:10.1038/365721a0 · Cited by 1,910 · see citing works
Evidence for alternating head catalysis by kinesin during microtubule-stimulated ATP hydrolysis.
Hackney, D. D. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.15.6865 · Cited by 351 · see citing works
Alternating Site Mechanism of the Kinesin ATPase
Gilbert, S. P., Moyer, M. L., & Johnson, K. A. (1998) · Biochemistry · doi:10.1021/bi971117b · Cited by 172 · see citing works
The kinetic cycles of myosin, kinesin, and dynein
Hackney, D. D. (1996) · Annual Review of Physiology · doi:10.1146/annurev.ph.58.030196.003503 · Cited by 118 · see citing works
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The Molecular Motor Toolbox for Intracellular Transport
Vale, R. D. (2003) · Cell · doi:10.1016/s0092-8674(03)00111-9 · Cited by 1,982 · see citing works
Self-citationYildiz, A., Tomishige, M., Vale, R. D., & Selvin, P. R. (2004) · Science · doi:10.1126/science.1093753 · Cited by 965 · see citing works
Self-citationCargo of kinesin identified as JIP scaffolding proteins and associated signaling molecules
Verhey, K. J., Meyer, D., Deehan, R., Blenis, J., Schnapp, B. J., Rapoport, T. A., & Margolis, B. (2001) · The Journal of Cell Biology · doi:10.1083/jcb.152.5.959 · Cited by 609 · see citing works
Chemomechanical coupling of the forward and backward steps of single kinesin molecules
Nishiyama, M., Higuchi, H., & Yanagida, T. (2002) · Nature Cell Biology · doi:10.1038/ncb857 · Cited by 327 · see citing works
Single-molecule investigation of the interference between kinesin, tau and MAP2c
Seitz, A., Kojima, H., Oiwa, K., Mandelkow, E.-M., Song, Y.-H., & Mandelkow, E. (2002) · The EMBO Journal · doi:10.1093/emboj/cdf503 · Cited by 257 · see citing works
Rosenfeld, S. S., Fordyce, P. M., Jefferson, G. M., King, P. H., & Block, S. M. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m300849200 · Cited by 188 · see citing works
Alternate fast and slow stepping of a heterodimeric kinesin molecule
Kaseda, K., Higuchi, H., & Hirose, K. (2003) · Nature Cell Biology · doi:10.1038/ncb1067 · Cited by 179 · see citing works
How kinesin waits between steps
Mori, T., Vale, R. D., & Tomishige, M. (2007) · Nature · doi:10.1038/nature06346 · Cited by 171 · see citing works
Self-citationForce Generation in Kinesin Hinges on Cover-Neck Bundle Formation
Hwang, W., Lang, M. J., & Karplus, M. (2008) · Structure · doi:10.1016/j.str.2007.11.008 · Cited by 161 · see citing works
Analysis of the migration behaviour of single microtubules in electric fields
Stracke, R., Böhm, K. J., Wollweber, L., Tuszynski, J. A., & Unger, E. (2002) · Biochemical and Biophysical Research Communications · doi:10.1016/s0006-291x(02)00251-6 · Cited by 147 · see citing works
Nucleotide-Dependent Single- to Double-Headed Binding of Kinesin
Kawaguchi, K., & Ishiwata, S. (2001) · Science · doi:10.1126/science.291.5504.667 · Cited by 132 · see citing works
Kikkawa, M., & Hirokawa, N. (2006) · The EMBO Journal · doi:10.1038/sj.emboj.7601299 · Cited by 125 · see citing works
Inhibition of kinesin motility by ADP and phosphate supports a hand-over-hand mechanism
Schief, W. R., Clark, R. H., Crevenna, A. H., & Howard, J. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0304369101 · Cited by 119 · see citing works
Entropy rectifies the Brownian steps of kinesin
Taniguchi, Y., Nishiyama, M., Ishii, Y., & Yanagida, T. (2005) · Nature Chemical Biology · doi:10.1038/nchembio741 · Cited by 116 · see citing works
A genomic toolkit to investigate kinesin and myosin motor function in cells
Maliga, Z., Junqueira, M., Toyoda, Y., Ettinger, A., Mora-Bermúdez, F., Klemm, R. W., Vasilj, A., Guhr, E., Ibarlucea-Benitez, I., Poser, I., et al. (2013) · Nature Cell Biology · doi:10.1038/ncb2689 · Cited by 103 · see citing works
Klumpp, L. M., Hoenger, A., & Gilbert, S. P. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0307691101 · Cited by 107 · see citing works
Lucky 13-microtubule depolymerisation by kinesin-13 motors
Moores, C. A., & Milligan, R. A. (2006) · Journal of Cell Science · doi:10.1242/jcs.03224 · Cited by 92 · see citing works
Self-citationATP Reorients the Neck Linker of Kinesin in Two Sequential Steps
Rosenfeld, S. S., Jefferson, G. M., & King, P. H. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m103899200 · Cited by 84 · see citing works
KIF1D is a fast non‐processive kinesin that demonstrates novel K‐loop‐dependent mechanochemistry
Rogers, K. R., Weiss, S., Crevel, I., Brophy, P. J., Geeves, M., & Cross, R. (2001) · The EMBO Journal · doi:10.1093/emboj/20.18.5101 · Cited by 73 · see citing works
Measuring Kinesin's First Step
Rosenfeld, S. S., Xing, J., Jefferson, G. M., Cheung, H. C., & King, P. H. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m205261200 · Cited by 54 · see citing works
All Wired Up: An Exploration of the Electrical Properties of Microtubules and Tubulin
Kalra, A. P., Eakins, B. B., Patel, S. D., Ciniero, G., Rezania, V., Shankar, K., & Tuszynski, J. A. (2020) · ACS Nano · doi:10.1021/acsnano.0c06945 · Cited by 49 · see citing works
Cohen, C., Mouterde, T., Quéré, D., & Clanet, C. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1419315112 · Cited by 40 · see citing works
Origin and Evolution of the Self-Organizing Cytoskeleton in the Network of Eukaryotic Organelles
Jekely, G. (2014) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a016030 · Cited by 38 · see citing works
Coordination between Motor Domains in Processive Kinesins
Sablin, E. P., & Fletterick, R. J. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.r300036200 · Cited by 32 · see citing works
Origin of Friction Derived from Rupture Dynamics
Suda, H. (2001) · Langmuir · doi:10.1021/la0106384 · Cited by 25 · see citing works
- Authors
- Vale, R. D., & Milligan, R. A.
- Affiliations
- Howard Hughes Medical Institute (US)
- Scripps Research Institute (US)
- Corresponding author
- Vale, Ronald D.
- ORCID
- Vale, Ronald D.
- Venue
- Science
- Published
- 2000-04-07
- Volume
- 288
- Issue
- 5463
- Pages
- 88-95
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2102145519
- Cited by
- 1,503
- References
- 4
- Citations by year
- 2026: 23 · 2025: 42 · 2024: 40 · 2023: 31 · 2022: 59 · 2021: 38 · 2020: 49 · 2019: 36 · 2018: 58 · 2017: 65 · 2016: 45 · 2015: 44 · 2014: 71 · 2013: 59 · 2012: 59
- FWCI
- 6.01
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiomyopathy and Myosin Studies
- Keywords
- molecular motors, kinesin, myosin, microtubule motility, actin-based motility, ATP hydrolysis, conformational change, mechanical amplifier, intracellular trafficking, cell division, muscle contraction
- MeSH terms
- Actins, Adenosine Triphosphate, Animals, Binding Sites, Cytoskeleton, Microtubules, Models, Biological, Models, Molecular, Myosins, Protein Conformation, Kinesins, Protein Structure, Secondary, Evolution, Molecular, Molecular Motor Proteins
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