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Cited by 455

The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.

Fluorescence-intensity distribution analysis and its application in biomolecular detection technology

Open access
Kask, Peet, Palo, Kaupo, Ullmann, Dirk, Gall, Karsten·1999·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.96.24.13756
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Abstract

A methodology, fluorescence-intensity distribution analysis, has been developed for confocal microscopy studies in which the fluorescence intensity of a sample with a heterogeneous brightness profile is monitored. An adjustable formula, modeling the spatial brightness distribution, and the technique of generating functions for calculation of theoretical photon count number distributions serve as the two cornerstones of the methodology. The method permits the simultaneous determination of concentrations and specific brightness values of a number of individual fluorescent species in solution. Accordingly, we present an extremely sensitive tool to monitor the interaction of fluorescently labeled molecules or other microparticles with their respective biological counterparts that should find a wide application in life sciences, medicine, and drug discovery. Its potential is demonstrated by studying the hybridization of 5′-(6-carboxytetramethylrhodamine)-labeled and nonlabeled complementary oligonucleotides and the subsequent cleavage of the DNA hybrids by restriction enzymes.

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Metadata

Authors
Kask, P., Palo, K., Ullmann, D., & Gall, K.
Venue
Proceedings of the National Academy of Sciences
Publisher
Proceedings of the National Academy of Sciences
Published
1999-11-23
Volume
96
Issue
24
Pages
13756-13761
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.96.24.13756
OpenAlex
W2073271190
Cited by
455
References
11
FWCI
12.50
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Fluorescence-intensity distribution analysis and its application in biomolecular detection technology. https://app.cochat.ai/papers/10.1073/pnas.96.24.13756
The paper
Kask, P., Palo, K., Ullmann, D., & Gall, K. (1999). Fluorescence-intensity distribution analysis and its application in biomolecular detection technology. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.96.24.13756

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.