The DOI matches its Crossref record; 58 of the 58 references it cites were found in a registry or an index.
Vascular Endothelial Growth Factor Increases Urokinase Receptor Expression in Vascular Endothelial Cells
Open accessAbstract
Vascular endothelial growth factor (VEGF) is a potent angiogenic factor and endothelial cell-specific mitogen that stimulates urokinase-type plasminogen activator (uPA) activity in vascular endothelial cells. Here, we report that VEGF increases the high affinity binding of uPA to the same cells and that this binding is prevented by a peptide corresponding to the uPA receptor (uPAR) binding growth factor-like domain of uPA. Ligand cross-linking, ligand blotting, and uPA-Sepharose affinity chromatography revealed an increase in a cell surface uPA binding protein that corresponds to the uPAR on the basis of its affinity for uPA, M(r) of 50,000-55,000, and phosphatidylinositol-specific phospholipase C sensitivity. By Scatchard analysis, VEGF increased the number of uPAR molecules by 2.8-3.5-fold and concomitantly decreased their affinity for uPA. By northern blotting uPAR mRNA was increased in a dose- and time-dependent manner in response to VEGF. Taken together, these findings demonstrate that VEGF-induced angiogenesis is accompanied by increased uPAR expression and uPA activity on the endothelial cell surface. These observations are consistent with the notion that the uPA-uPAR interaction facilitates cellular invasion.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
Chomczynski, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1016/0003-2697(87)90021-2 · Cited by 46,426
Folkman, J., & Klagsbrun, M. (1987) · Science · doi:10.1126/science.2432664 · Cited by 3,491
Molecular and Biological Properties of the Vascular Endothelial Growth Factor Family of Proteins
FERRARA, N., HOUCK, K., JAKEMAN, L., & LEUNG, D. W. (1992) · Endocrine Reviews · doi:10.1210/edrv-13-1-18 · Cited by 1,655
Self-citationAusprunk, D. H., & Folkman, J. (1977) · Microvascular Research · doi:10.1016/0026-2862(77)90141-8 · Cited by 1,281
Biology and biochemistry of proteinases in tumor invasion
Mignatti, P., & Rifkin, D. B. (1993) · Physiological Reviews · doi:10.1152/physrev.1993.73.1.161 · Cited by 1,244
Self-citationThe plasminogen activator/plasmin system.
Vassalli, J. D., Sappino, A. P., & Belin, D. (1991) · Journal of Clinical Investigation · doi:10.1172/jci115405 · Cited by 1,164
Self-citationPhysiological consequences of loss of plasminogen activator gene function in mice
Carmeliet, P., Schoonjans, L., Kieckens, L., Ream, B., Degen, J., Bronson, R., De Vos, R., van den Oord, J. J., Collen, D., & Mulligan, R. C. (1994) · Nature · doi:10.1038/368419a0 · Cited by 1,037
Klagsbrun, M., & D'Amore, P. A. (1991) · Annual Review of Physiology · doi:10.1146/annurev.ph.53.030191.001245 · Cited by 957
Pepper, M. S., Ferrara, N., Orci, L., & Montesano, R. (1992) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(92)92277-5 · Cited by 858
Self-citationA cellular binding site for the Mr 55,000 form of the human plasminogen activator, urokinase.
Vassalli, J. D., Baccino, D., & Belin, D. (1985) · The Journal of cell biology · doi:10.1083/jcb.100.1.86 · Cited by 712
Self-citation
Cited by
The Biology of Vascular Endothelial Growth Factor
Ferrara, N., & Davis-Smyth, T. (1997) · Endocrine Reviews · doi:10.1210/edrv.18.1.0287 · Cited by 4,330
Self-citationVEGF as a Key Mediator of Angiogenesis in Cancer
Carmeliet, P. (2005) · Oncology · doi:10.1159/000088478 · Cited by 1,768
Continuous and Discrete Mathematical Models of Tumor-induced Angiogenesis
Anderson, A. (1998) · Bulletin of Mathematical Biology · doi:10.1006/bulm.1998.0042 · Cited by 1,108
Oh, S. P., Seki, T., Goss, K. A., Imamura, T., Yi, Y., Donahoe, P. K., Li, L., Miyazono, K., ten Dijke, P., Kim, S., et al. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.6.2626 · Cited by 851
Endothelial Dysfunction in Pulmonary Hypertension
Budhiraja, R., Tuder, R. M., & Hassoun, P. M. (2004) · Circulation · doi:10.1161/01.cir.0000102381.57477.50 · Cited by 768
Metadata
- Authors
- Mandriota, S. J., Seghezzi, G., Vassalli, J.-D., Ferrara, N., Wasi, S., Mazzieri, R., Mignatti, P., & Pepper, M. S.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1995-04
- Volume
- 270
- Issue
- 17
- Pages
- 9709-9716
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1967365012
- Cited by
- 267
- References
- 58
- FWCI
- 10.50
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Vascular Endothelial Growth Factor Increases Urokinase Receptor Expression in Vascular Endothelial Cells. https://app.cochat.ai/papers/10.1074/jbc.270.17.9709
- The paper
- Mandriota, S. J., Seghezzi, G., Vassalli, J.-D., Ferrara, N., Wasi, S., Mazzieri, R., Mignatti, P., & Pepper, M. S. (1995). Vascular Endothelial Growth Factor Increases Urokinase Receptor Expression in Vascular Endothelial Cells. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.270.17.9709
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
