The DOI matches its Crossref record; 67 of the 67 references it cites were found in a registry or an index.
Glyceraldehyde-3-phosphate Dehydrogenase Selectively Binds AU-rich RNA in the NAD+-binding Region (Rossmann Fold)
Open accessAbstract
A 36-kDa protein that binds AU-rich RNA was purified from human spleen and identified as glyceraldehyde-3-phosphate dehydrogenase (GAPDH). GAPDH has been previously demonstrated to bind tRNA with high affinity. Competition studies suggested that cytoplasmic GAPDH binds the AU-rich elements (AREs) of lymphokine mRNA 3'-untranslated regions with higher affinity than tRNA. The AUUUA-specific RNA binding activity of GAPDH was inhibited by NAD+, NADH, and ATP in a concentration-dependent manner, suggesting that RNA binding of GAPDH might involve the NAD(+)-binding region, or dinucleotide-binding (Rossmann) fold. This hypothesis was supported by experiments that localized RNA binding to the predicted N-terminal 6.8-kDa peptide, known to be involved in the formation of the NAD(+)-binding domain. The direct demonstration of ARE-specific binding protein activity localized to the NAD(+)-binding region of GAPDH supports the general concept that enzymes containing this domain may exhibit specific RNA binding activity and play additional roles in nucleic acid metabolism. Finally, cytoplasmic GAPDH was found in the polysomal fraction of T lymphocytes. Thus, the RNA binding specificity of GAPDH as well as its localization within the cell merit its strong consideration as a protein important in the regulation of ARE-dependent mRNA turnover and translation in addition to its well described role in glycolysis.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“GAPDH is a multifunctional enzyme that can bind a range of RNAs, including AU-rich regions in the 3’ untranslated region (UTR) of cytokine mRNAs, including IFN-γ and IL-2 (111).”
From Fueling Immunity: Insights into Metabolism and Lymphocyte Function (2013) · cites this paper as [111]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Schägger, H., & von Jagow, G. (1987) · Analytical Biochemistry · doi:10.1016/0003-2697(87)90587-2 · Cited by 11,420
Shaw, G. D., & Kamen, R. (1986) · Cell · doi:10.1016/0092-8674(86)90341-7 · Cited by 3,946
hnRNP PROTEINS AND THE BIOGENESIS OF mRNA
Dreyfuss, G., Matunis, M. J., Piñol-Roma, S., & Burd, C. G. (1993) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.62.070193.001445 · Cited by 1,558
Regulating the fate of mRNA: the control of cellular iron metabolism
Klausner, R. D., Rouault, T. A., & Harford, J. B. (1993) · Cell · doi:10.1016/0092-8674(93)90046-s · Cited by 1,210
Wierenga, R. K., Terpstra, P., & Hol, W. G. J. (1986) · Journal of Molecular Biology · doi:10.1016/0022-2836(86)90409-2 · Cited by 1,159
Shuttling of pre-mRNA binding proteins between nucleus and cytoplasm
Piñol-Roma, S., & Dreyfuss, G. (1992) · Nature · doi:10.1038/355730a0 · Cited by 842
Alitalo, K., Schwab, M., Lin, C. C., Varmus, H. E., & Bishop, J. M. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.6.1707 · Cited by 761
Staphylococcal Protease: A Proteolytic Enzyme Specific for Glutamoyl Bonds
Houmard, J., & Drapeau, G. R. (1972) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.69.12.3506 · Cited by 702
Expression of human immune interferon cDNA in E. coli and monkey cells
Gray, P. W., Leung, D. W., Pennica, D., Yelverton, E., Najarian, R. C., Simonsen, C. C., Derynck, R., Sherwood, P. J., Wallace, D. M., Berger, S. L., et al. (1982) · Nature · doi:10.1038/295503a0 · Cited by 691
Research works printed but not linked: 8
- Jones, 1987, Mol. Cell. Biol., 7, 4513
- Bohjanen, 1991, Mol. Cell. Biol., 11, 3288
- Vakalopoulou, 1991, Mol. Cell. Biol., 11, 3355
- Brewer, 1991, Mol. Cell. Biol., 11, 2460
- Levine, 1993, Mol. Cell. Biol., 13, 3494
- Zhang, 1993, Mol. Cell. Biol., 13, 7652
- Aharon, 1993, Mol. Cell. Biol., 13, 1971
- Grafi, 1993, Mol. Cell. Biol., 13, 3487
Cited by
Sorted by citations
Posttranscriptional Control of T Cell Effector Function by Aerobic Glycolysis
Chang, C.-H., Curtis, J. D., Maggi, L. B., Faubert, B., Villarino, A. V., O’Sullivan, D., Huang, S. C.-C., van der Windt, G. J. W., Blagih, J., Qiu, J., et al. (2013) · Cell · doi:10.1016/j.cell.2013.05.016 · Cited by 2,204
Insights into RNA Biology from an Atlas of Mammalian mRNA-Binding Proteins
Castello, A., Fischer, B., Eichelbaum, K., Horos, R., Beckmann, B. M., Strein, C., Davey, N. E., Humphreys, D. T., Preiss, T., Steinmetz, L. M., et al. (2012) · Cell · doi:10.1016/j.cell.2012.04.031 · Cited by 2,132
AU-rich elements: characterization and importance in mRNA degradation
Chen, C.-Y. A., & Shyu, A.-B. (1995) · Trends in Biochemical Sciences · doi:10.1016/s0968-0004(00)89102-1 · Cited by 1,948
A brave new world of RNA-binding proteins
Hentze, M. W., Castello, A., Schwarzl, T., & Preiss, T. (2018) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2017.130 · Cited by 1,821
Fueling Immunity: Insights into Metabolism and Lymphocyte Function
Pearce, E. L., Poffenberger, M. C., Chang, C.-H., & Jones, R. G. (2013) · Science · doi:10.1126/science.1242454 · Cited by 1,363
Metadata
- Authors
- Nagy, E., & Rigby, W. F. C.
- Affiliations
- White River Junction VA Medical Center (US)
- Corresponding author
- Rigby, William F.C.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1995-02
- Volume
- 270
- Issue
- 6
- Pages
- 2755-2763
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2050843429
- Cited by
- 361
- References
- 67
- Citations by year
- 2026: 5 · 2025: 14 · 2024: 10 · 2023: 7 · 2022: 5 · 2021: 4 · 2020: 18 · 2019: 9 · 2018: 15 · 2017: 10 · 2016: 12 · 2015: 7 · 2014: 12 · 2013: 11 · 2012: 14
- FWCI
- 6.32
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5k04ai000910-02)
- National Institute of Allergy and Infectious Diseases (KO4 AI00910, R0 1 AI2434)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
- Keywords
- glyceraldehyde-3-phosphate dehydrogenase, NAD-binding domain, Rossmann fold, RNA-binding proteins, tRNA binding, 3' untranslated region, mRNA turnover, translational regulation, T cells, polysomes
- MeSH terms
- Adenine, Base Sequence, Cells, Cultured, Glyceraldehyde-3-Phosphate Dehydrogenases, Humans, Molecular Sequence Data, NAD, Protein Binding, RNA, Substrate Specificity, T-Lymphocytes, Uridine, Protein Folding
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
