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Transforming Growth Factor-β Stimulates the Production of Osteoprotegerin/Osteoclastogenesis Inhibitory Factor by Bone Marrow Stromal Cells
Open accessAbstract
Osteoprotegerin (OPG)/osteoclastogenesis inhibitory factor (OCIF) is a recently identified cytokine that belongs to the tumor necrosis factor receptor superfamily and regulates bone mass by inhibiting osteoclastic bone resorption. The present study was undertaken to determine whether OPG/OCIF is produced in bone microenvironment and how the expression is regulated. A transcript for OPG/OCIF at 3.1 kilobases was detected in bone marrow stromal cells (ST2 and MC3T3-G2/PA6) as well as in osteoblastic cells (MC3T3-E1). Transforming growth factor-beta1 (TGF-beta1) markedly increased the steady-state level of OPG/OCIF mRNA in a dose-dependent manner, while TGF-beta1 suppressed the mRNA expression of tumor necrosis factor-related activation-induced cytokine (TRANCE)/receptor activator of NF-kappaB ligand (RANKL), a positive regulator of osteoclastogenesis to which OPG/OCIF binds. The effect of TGF-beta1 on the expression of OPG/OCIF mRNA was transient, with a peak level at 3-6 h. The up-regulation of OPG/OCIF mRNA by TGF-beta1 in ST2 cells did not require de novo protein synthesis and involved both a transcriptional and a post-transcriptional mechanism. Western blot analysis and an enzyme-linked immunosorbent assay revealed that TGF-beta1 significantly increased the secretion of OPG/OCIF protein by ST2 cells at 6-24 h. In murine bone marrow cultures, TGF-beta1 markedly inhibited the formation of tartrate-resistant acid phosphatase-positive multinucleated osteoclast-like cells in the presence of 1,25-dihydroxyvitamin D3, whose effect was significantly reversed by a neutralizing antibody against OPG/OCIF. These results suggest that TGF-beta1 negatively regulates osteoclastogenesis, at least in part, through the induction of OPG/OCIF by bone marrow stromal cells and that the balance between OPG/OCIF and TRANCE/RANKL in local environment may be an important determinant of osteoclastic bone resorption.
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“High TGF-β1 expression inhibits the migration of osteoclast precursors208 by suppressing RANKL expression and by stimulating OPG production in osteoblasts.209”
From Bone remodeling: an operational process ensuring survival and bone mechanical competence (2022) · cites this paper as [209]
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Cites
Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction
Chomczynski, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1006/abio.1987.9999 · Cited by 65,515
Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation.
Lacey, D. L., Timms, E., Tan, H.-L., Kelley, M. J., Dunstan, C. R., Burgess, T., Elliott, R., Colombero, A., Elliott, G., Scully, S., et al. (1998) · Cell · doi:10.1016/s0092-8674(00)81569-x · Cited by 5,439
Osteoprotegerin: a novel secreted protein involved in the regulation of bone density.
Simonet, W. S., Lacey, D. L., Dunstan, C. R., Kelley, M., Chang, M.-S., Lüthy, R., Nguyen, H. Q., Wooden, S., Bennett, L., Boone, T., et al. (1997) · Cell · doi:10.1016/s0092-8674(00)80209-3 · Cited by 5,130
Yasuda, H., Shima, N., Nakagawa, N., Yamaguchi, K., Kinosaki, M., Mochizuki, S.-I., Tomoyasu, A., Yano, K., Goto, M., Murakami, A., et al. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.7.3597 · Cited by 4,224
Self-citationA homologue of the TNF receptor and its ligand enhance T-cell growth and dendritic-cell function.
Anderson, D. M., Maraskovsky, E., Billingsley, W. L., Dougall, W. C., Tometsko, M. E., Roux, E. R., Teepe, M. C., DuBose, R. F., Cosman, D., & Galibert, L. (1997) · Nature · doi:10.1038/36593 · Cited by 2,290
Targeted disruption of the c-src proto-oncogene leads to osteopetrosis in mice
Soriano, P. M., Montgomery, C. A., Geske, R. S., & Bradley, A. (1991) · Cell · doi:10.1016/0092-8674(91)90499-o · Cited by 1,997
Yoshida, H., Hayashi, S.-I., Kunisada, T., Ogawa, M., Nishikawa, S., Okamura, H., Sudo, T., Shultz, L. D., & Nishikawa, S.-I. (1990) · Nature · doi:10.1038/345442a0 · Cited by 1,789
Sudo, H., Kodama, H. A., Amagai, Y., Yamamoto, S., & Kasai, S. (1983) · The Journal of Cell Biology · doi:10.1083/jcb.96.1.191 · Cited by 1,673
Manolagas, S. C., & Jilka, R. L. (1995) · New England Journal of Medicine · doi:10.1056/nejm199502023320506 · Cited by 1,670
c-Fos: a Key Regulator of Osteoclast-Macrophage Lineage Determination and Bone Remodeling
Grigoriadis, A. E., Wang, Z., Cecchini, M. G., Hofstetter, W., Felix, R., Fleisch, H. A., & Wagner, E. F. (1994) · Science · doi:10.1126/science.7939685 · Cited by 1,264
Yasuda, H., Shima, N., Nakagawa, N., Mochizuki, S.-I., Yano, K., Fujise, N., Sato, Y., Goto, M., Yamaguchi, K., Kuriyama, M., et al. (1998) · Endocrinology · doi:10.1210/endo.139.3.5837 · Cited by 1,201
Self-citationPycnodysostosis, a Lysosomal Disease Caused by Cathepsin K Deficiency
Gelb, B. D., Shi, G., Chapman, H. A., & Desnick, R. J. (1996) · Science · doi:10.1126/science.273.5279.1236 · Cited by 1,022
Requirement for NF-κB in osteoclast and B-cell development
Franzoso, G., Carlson, L., Xing, L., Poljak, L., Shores, E. W., Brown, K. D., Leonardi, A., Tran, T., Boyce, B. F., & Siebenlist, U. (1997) · Genes & Development · doi:10.1101/gad.11.24.3482 · Cited by 996
Osteopetrosis in mice lacking NF-κB1 and NF-κB2
Iotsova, V., Caamaño, J., Loy, J. K., Yang, Y., Lewin, A. C., & Bravo, R. (1997) · Nature Medicine · doi:10.1038/nm1197-1285 · Cited by 990
TGFβ Signaling: Receptors, Transducers, and Mad Proteins
Massagué, J. (1996) · Cell · doi:10.1016/s0092-8674(00)81296-9 · Cited by 836
Isolation of a novel cytokine from human fibroblasts that specifically inhibits osteoclastogenesis.
Tsuda, E., Goto, M., Mochizuki, S.-I., Yano, K., Kobayashi, F., Morinaga, T., & Higashio, K. (1997) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1997.6603 · Cited by 787
Self-citationEstrogen, cytokines, and pathogenesis of postmenopausal osteoporosis
Pacifici, R. (1996) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.5650110802 · Cited by 741
Joyce, M. E., Roberts, A. B., Sporn, M. B., & Bolander, M. E. (1990) · The Journal of Cell Biology · doi:10.1083/jcb.110.6.2195 · Cited by 732
Takahashi, N., Yamana, H., Yoshiki, S., Roodman, G. D., Mundy, G. R., Jones, S. J., BOYDE, A., & Suda, T. (1988) · Endocrinology · doi:10.1210/endo-122-4-1373 · Cited by 731
In VivoStimulation of Bone Formation by Transforming Growth Factor-β
Noda, M., & Camilliere, J. J. (1989) · Endocrinology · doi:10.1210/endo-124-6-2991 · Cited by 582
Advances in Bone Biology: The Osteoclast*
Roodman, G. D. (1996) · Endocrine Reviews · doi:10.1210/edrv-17-4-308 · Cited by 553
The Nonamer UUAUUUAUU Is the Key AU-Rich Sequence Motif That Mediates mRNA Degradation
Zubiaga, A. M., Belasco, J. G., & Greenberg, M. E. (1995) · Molecular and Cellular Biology · doi:10.1128/mcb.15.4.2219 · Cited by 543
Osteopetrosis in mice lacking haematopoietic transcription factor PU.1
Tondravi, M. M., McKercher, S. R., Anderson, K., Erdmann, J. M., Quiroz, M. E., Maki, R. A., & Teitelbaum, S. L. (1997) · Nature · doi:10.1038/386081a0 · Cited by 537
Transforming Growth Factor-β Gene Family Members and Bone*
Horowitz, M. C., Wozney, J. M., & McCarthy, T. L. (1994) · Endocrine Reviews · doi:10.1210/edrv-15-1-27 · Cited by 496
Udagawa, N., Takahashi, N., Akatsu, T., Sasaki, T., Yamaguchi, A., KODAMA, H., Martin, T. J., & Suda, T. (1989) · Endocrinology · doi:10.1210/endo-125-4-1805 · Cited by 492
Regulation of Osteoclast Function
Suda, T., Nakamura, I., Jimi, E., & Takahashi, N. (1997) · Journal of Bone and Mineral Research · doi:10.1359/jbmr.1997.12.6.869 · Cited by 404
Tashjian, A. H., Voelkel, E. F., Lazzaro, M., Singer, F. R., Roberts, A. B., Derynck, R., Winkler, M. E., & Levine, L. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.13.4535 · Cited by 381
Chenu, C., Pfeilschifter, J., Mundy, G. R., & Roodman, G. D. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.15.5683 · Cited by 295
Ogawa, M., Nishikawa, S.-I., Ikuta, K., Yamamura, F., Naito, M., Takahashi, K., & Nishikawa, S.-I. (1988) · The EMBO Journal · doi:10.1002/j.1460-2075.1988.tb02949.x · Cited by 281
Transforming growth factor beta inhibits bone resorption in fetal rat long bone cultures.
Pfeilschifter, J., Seyedin, S. M., & Mundy, G. R. (1988) · Journal of Clinical Investigation · doi:10.1172/jci113647 · Cited by 261
Characterization of Structural Domains of Human Osteoclastogenesis Inhibitory Factor
Yamaguchi, K., Kinosaki, M., Goto, M., Kobayashi, F., Tsuda, E., Morinaga, T., & Higashio, K. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.9.5117 · Cited by 200
Self-citationWatanabe, K., Yamada, H., & Yamaguchi, Y. (1995) · The Journal of Cell Biology · doi:10.1083/jcb.130.5.1207 · Cited by 154
Self-citationKodama, H., Amagai, Y., Koyama, H., & Kasai, S. (1982) · Journal of Cellular Physiology · doi:10.1002/jcp.1041120114 · Cited by 136
SHINAR, D. M., & Rodan, G. A. (1990) · Endocrinology · doi:10.1210/endo-126-6-3153 · Cited by 129
Dieudonné, S. C., Foo, P., van Zoelen, E. J. J., & Burger, E. H. (1991) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.5650060509 · Cited by 87
Effects of transforming growth factor β1 on the regulation of osteoclastic development and function
Hattersley, G., & Chambers, T. J. (1991) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.5650060210 · Cited by 75
The complete cDNA sequence of mouse elongation factor 1 alpha (EF 1 alpha) mRNA
Lu, X., & Werner, D. (1989) · Nucleic Acids Research · doi:10.1093/nar/17.1.442 · Cited by 48
Research works printed but not linked: 3
- Roodman, 1996, Endocr. Rev., 17, 308
- Chomczynski, 1987, Anal. Biochem., 162, 156, 10.1016/0003-2697(87)90021-2 · DOI
- Centrella, 1994, Endocr. Rev., 15, 27
Cited by
Sorted by citations
Minireview: The OPG/RANKL/RANK System
Khosla, S. (2001) · Endocrinology · doi:10.1210/endo.142.12.8536 · Cited by 1,515
RANK is essential for osteoclast and lymph node development.
Dougall, W. C., Glaccum, M., Charrier, K., Rohrbach, K., Brasel, K., De Smedt, T., Daro, E., Smith, J., Tometsko, M. E., Maliszewski, C. R., et al. (1999) · Genes & Development · doi:10.1101/gad.13.18.2412 · Cited by 1,478
Autologous platelets as a source of proteins for healing and tissue regeneration
Anitua, E., Andı́a, I., Ardanza, B., Nurden, P., & Nurden, A. T. (2003) · Thrombosis and Haemostasis · doi:10.1160/th03-07-0440 · Cited by 1,496
Hofbauer, L. C., Khosla, S., Dunstan, C. R., Lacey, D. L., Boyle, W. J., & Riggs, B. L. (2000) · Journal of Bone and Mineral Research · doi:10.1359/jbmr.2000.15.1.2 · Cited by 1,185
Changes in Proinflammatory Cytokine Activity after Menopause
Pfeilschifter, J., Köditz, R., Pfohl, M., & Schatz, H. (2002) · Endocrine Reviews · doi:10.1210/edrv.23.1.0456 · Cited by 929
Metadata
- Authors
- Takai, H., Kanematsu, M., Yano, K., Tsuda, E., Higashio, K., Ikeda, K., Watanabe, K., & Yamada, Y.
- Affiliations
- Megmilk Snow Brand (Japan) (JP)
- Corresponding author
- Yamada, Yoshiji
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1998-10
- Volume
- 273
- Issue
- 42
- Pages
- 27091-27096
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1994756823
- Cited by
- 299
- References
- 38
- Citations by year
- 2026: 2 · 2025: 6 · 2024: 4 · 2023: 2 · 2022: 4 · 2021: 5 · 2020: 3 · 2019: 6 · 2018: 3 · 2017: 6 · 2016: 6 · 2015: 7 · 2014: 4 · 2013: 6 · 2012: 8
- FWCI
- 7.44
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › TGF-β signaling in diseases
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
- Keywords
- osteoprotegerin, TGF-β1, mesenchymal stem cells, RANKL, osteoclastogenesis, bone resorption, osteoblasts, transcriptional regulation, post-transcriptional regulation, osteoclast-like cells, 1,25-dihydroxyvitamin D3
- MeSH terms
- Animals, Bone Marrow Cells, Cell Differentiation, Cell Line, Dose-Response Relationship, Drug, Gene Expression Regulation, Glycoproteins, Models, Biological, Osteoclasts, RNA, Messenger, Transforming Growth Factor beta, Stromal Cells, Receptors, Tumor Necrosis Factor, Receptors, Cytoplasmic and Nuclear, Mice, Osteoprotegerin
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