The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
The Changes in Adenine Nucleotides Measured in Glucose-stimulated Rodent Islets Occur in β Cells but Not in α Cells and Are Also Observed in Human Islets
Open accessAbstract
Glucose metabolism by pancreatic beta and alpha cells is essential for stimulation of insulin secretion and inhibition of glucagon secretion. Studies using rodent islets have suggested that the ATP/ADP ratio serves as second messenger in beta cells. This study compared the effects of glucose on glucose oxidation ([U-14C]glucose) and adenine nucleotides (luminometric method) in purified rat alpha and beta cells. The rate of glucose oxidation at 1 mM glucose was higher in beta than alpha cells (4.5-fold, i.e. approximately 2-fold after normalization for cell size). It was more strongly stimulated by 10 mM glucose in beta cells (9-fold) than in alpha cells (5-fold). At 1 mM glucose, ATP levels were similar in both cell types, which corresponds to an approximately 2-fold higher concentration in alpha cells ( approximately 6.5 mM) than in beta cells ( approximately 3 mM). In beta cells, glucose dose-dependently increased ATP and decreased ADP levels, causing a rise in the ATP/ADP ratio from 2.4 to 11.6 at 1 and 10 mM, respectively. In alpha cells, glucose did not affect ATP and ADP levels, and the ATP/ADP ratio remained stable around 7.5. In human islets, the ATP/ADP ratio progressively increased between 1 and 10 mM glucose. In duct cells, which often contaminate human islet preparations, an increase in the ATP/ADP ratio sometimes occurred between 1 and 3 mM glucose. In conclusion, the present observations establish that the regulation of glucagon secretion by glucose does not involve changes in alpha cell adenine nucleotides and further support the role of the ATP/ADP ratio in the control of insulin secretion.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“These values are in broad agreement with biochemical measurements of [ATP]i in purified rat β-cells which suggest ATP is ~2 mM under basal conditions and rises to 4 mM when glucose is increased to 10 mM (142) (see v).”
From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [142]
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
A Lesson in Metabolic Regulation Inspired by the Glucokinase Glucose Sensor Paradigm
Matschinsky, F. (1996) · Diabetes · doi:10.2337/diab.45.2.223 · Cited by 721
METABOLIC COUPLING FACTORS IN PANCREATIC β-CELL SIGNAL TRANSDUCTION
Newgard, C. B., & McGarry, J. (1995) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.64.070195.003353 · Cited by 547
Gembal, M., Gilon, P., & Henquin, J. C. (1992) · Journal of Clinical Investigation · doi:10.1172/jci115714 · Cited by 494
Self-citationMechanism of glucose-induced insulin secretion.
Hedeskov, C. J. (1980) · Physiological Reviews · doi:10.1152/physrev.1980.60.2.442 · Cited by 435
Metabolic Fate of Glucose in Purified Islet Cells
Schuit, F., De Vos, A., Farfari, S., Moens, K., Pipeleers, D., Brun, T., & Prentki, M. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.30.18572 · Cited by 421
Self-citationA New in Vitro Model for the Study of Pancreatic A and B Cells*
PIPELEERS, D. G., Veld, P. I. '., Winkel, M. V. D., Maes, E., Schuit, F. C., & Gepts, W. (1985) · Endocrinology · doi:10.1210/endo-117-3-806 · Cited by 341
Self-citationInterrelationship of islet metabolism, adenosine triphosphate content and insulin release
Ashcroft, S. J. H., Weerasinghe, L., & Randle, P. J. (1973) · Biochemical Journal · doi:10.1042/bj1320223 · Cited by 301
Gembal, M., Detimary, P., Gilon, P., Gao, Z. Y., & Henquin, J. C. (1993) · Journal of Clinical Investigation · doi:10.1172/jci116308 · Cited by 260
Self-citationStimulus-secretion coupling in pancreatic β cells
Ashcroft, F. M., Proks, P., Smith, P. A., Ämmälä, C., Bokvist, K., & Rorsman, P. (1994) · Journal of Cellular Biochemistry · doi:10.1002/jcb.240550007 · Cited by 255
Endocrine Pancreas: Three-Dimensional Reconstruction Shows Two Types of Islets of Langerhans
Baetens, D., MALAISSE-LAGAE, F., Perrelet, A., & Orci, L. (1979) · Science · doi:10.1126/science.390711 · Cited by 237
Flagged first, then by citations
Research works printed but not linked: 5
- Wollheim, 1996, Diabetes Rev., 4, 276
- Detimary, 1996, Endocrinology, 137, 4671, 10.1210/endo.137.11.8895332 · DOI
- Ronner, 1991, Diabetes, 40 (Suppl. 1), 155
- Bokvist, 1998, Diabetologia, 41 (Suppl. 1), 139
- Hellman, 1969, Diabetologia, 37 (Suppl. 2), 11
Cited by
Sorted by citations
Items 101–158 of 158
A Novel ABCC8 (SUR1)-dependent Mechanism of Metabolism-Excitation Uncoupling
Babenko, A. P. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.c700243200 · Cited by 26
Proks, P., de Wet, H., & Ashcroft, F. M. (2014) · Journal of General Physiology · doi:10.1085/jgp.201411222 · Cited by 22
“It has been proposed that MgADP regulation of sulfonylurea inhibition may result in drug action varying with the metabolism of the cell, as MgADP levels rise when metabolism is low (Ghosh et al., 1991; Detimary et al., 1998; Ronner et al., 2001).”
BackgroundJonkers, F. C., Guiot, Y., Rahier, J., & Henquin, J. (2001) · British Journal of Pharmacology · doi:10.1038/sj.bjp.0704108 · Cited by 23
“As a result, the ATP : ADP ratio might be di erent (Detimary et al., 1998) and the K+-ATP channels closed to di erent extents by glucose.”
BackgroundSelf-citationGlycemic Control of Apoptosis in the Pancreatic Beta Cell: Danger of Extremes?
Martens, G. A., & de Casteele, M. V. (2007) · Antioxidants and Redox Signaling · doi:10.1089/ars.2006.1466 · Cited by 21
Høy, M., Bokvist, K. B., Xiao-Gang, W., Hansen, J. B., Juhl, K., Berggren, P., Buschard, K., & Gromada, J. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m007562200 · Cited by 22
Antidiabetic sulfonylurea stimulates insulin secretion independently of plasma membrane KATPchannels
Geng, X., Li, L., Bottino, R., Balamurugan, A. N., Bertera, S., Densmore, E., Su, A.-J. A., Chang, Y., Trucco, M., & Drain, P. F. (2007) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00016.2007 · Cited by 21
The KATP Channel and Neonatal Diabetes
Shimomura, K. (2009) · Endocrine Journal · doi:10.1507/endocrj.k08e-160 · Cited by 20
“Increased glycolysis induced by high blood glucose levels leads to changes in cytosolic concentrations of adenosine nucleotides such as ATP and ADP [23, 24].”
BackgroundInfluentialEffects of Periodic Intensive Insulin Therapy: An Updated Review
Dong, S., Lau, H., Chavarria, C., Alexander, M., Cimler, A., Elliott, J. P., Escovar, S. X., Lewin, J., Novak, J., & Lakey, J. R. T. (2019) · Current Therapeutic Research · doi:10.1016/j.curtheres.2019.04.003 · Cited by 20
Capillary LC−MS for High Sensitivity Metabolomic Analysis of Single Islets of Langerhans
Ni, Q., Reid, K. R., Burant, C., & Kennedy, R. T. (2008) · Analytical Chemistry · doi:10.1021/ac800406f · Cited by 20
RetractedLawandi, J., Tao, C., Ren, B., Williams, P. F., Ling, D., Swan, M. A., Nassif, N. T., Torpy, F. R., O’Brien, B. A., & Simpson, A. M. (2015) · Molecular Therapy — Methods & Clinical Development · doi:10.1038/mtm.2015.11 · Cited by 17
Galvanovskis, J., Braun, M., & Rorsman, P. (2011) · Interface Focus · doi:10.1098/rsfs.2010.0006 · Cited by 17
The α-Cell Conundrum: ATP-Sensitive K+ Channels and Glucose Sensing
Jacobson, D. A., Wicksteed, B. L., & Philipson, L. H. (2009) · Diabetes · doi:10.2337/db08-1618 · Cited by 19
“Levels of NAD(P)H in -cells were found to respond to glucose, as expected; however, -cell NAD(P)H levels did not vary substantially, thereby corroborating previous -cell studies in which equivalent ATP-to-ADP ratios and NAD(P)H or FAD fluorescence under low- and high-glucose conditions were observed (16,17).”
ResultReed, J., Bain, S. C., & Kanamarlapudi, V. (2024) · Diabetes Metabolic Syndrome and Obesity · doi:10.2147/dmso.s415934 · Cited by 21
“53,55 It is known that both phases of insulin secretion are ATP-dependent processes.”
ATP Regulates Sodium Channel Kinetics in Pancreatic Islet Beta Cells
Zou, N., Wu, X., Jin, Y., He, M.-Z., Wang, X.-X., Su, L.-D., Rupnik, M., Wu, Z.-Y., Liang, L., & Shen, Y. (2013) · Journal of Membrane Biology · doi:10.1007/s00232-012-9506-7 · Cited by 20
Stamenkovic, J., Andersson, L. E., Adriaenssens, A. E., Bagge, A., Sharoyko, V. V., Gribble, F. M., Reimann, F. M., Wollheim, C. B., Mulder, H., & Spégel, P. (2015) · Biochemical Journal · doi:10.1042/bj20140697 · Cited by 19
Modelling of energy-driven switch for glucagon and insulin secretion
Grubelnik, V., Zmazek, J., Markovič, R., Gosak, M., & Marhl, M. (2020) · Journal of Theoretical Biology · doi:10.1016/j.jtbi.2020.110213 · Cited by 15
Driggers, C., & Shyng, S. (2021) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/bs.mie.2021.02.008 · Cited by 16
Liu, W., Aerbajinai, W., Li, H., Liu, Y., Gavrilova, O., Jain, S., & Rodgers, G. P. (2018) · Endocrinology · doi:10.1210/en.2018-00451 · Cited by 14
Pancreatic islet cells: A model for calcium‐dependent peptide release
Soria, B., Tudurí, E., González, A., Hmadcha, A., Martín, F., Nadal, Á., & Quesada, I. (2010) · HFSP journal · doi:10.2976/1.3364560 · Cited by 15
“All these information about the -cell glucose metabolism agrees with the fact that ATP/ADP changes in response to glucose are almost invariable in -cells compared to the situation of -cells (Detimary et al., 1998).”
ResultAmino acids and the changing face of the α-cell
Hamilton, A., Eliasson, L., & Knudsen, J. G. (2023) · Peptides · doi:10.1016/j.peptides.2023.171039 · Cited by 14
Babenko, A. P., & Vaxillaire, M. (2011) · FEBS Letters · doi:10.1016/j.febslet.2011.10.020 · Cited by 13
“We compared the SU inhibition of L213R vs WT KATP activity under non-stimulatory conditions, as well as in the presence of the lowest possible submembrane [MgATP] and the highest possible [MgADP] in resting b-cells [32,33], as we did earlier for A type mutant KATP [7,11].”
MethodA computational systems analysis of factors regulating α cell glucagon secretion
Fridlyand, L. E., & Philipson, L. H. (2012) · Islets · doi:10.4161/isl.22193 · Cited by 12
“9,10,26 The NAD(P)H signal was greater in mouse islet α -cells compared with β -cells at 1 mM glucose, however, the increase of this signal with glucose was less in α -cells.”
BackgroundEvidence against a Ca2+-induced potentiation of dehydrogenase activity in pancreatic beta-cells
Drews, G., Bauer, C., Edalat, A., Düfer, M., & Krippeit-Drews, P. (2015) · Pflügers Archiv: European Journal of Physiology · doi:10.1007/s00424-015-1707-3 · Cited by 12
Effect of Creatine on the Pancreatic ß-Cell
Ročić, B., Lovrenčić, M., Poje, M., & Ashcroft, S. (2007) · Experimental and Clinical Endocrinology & Diabetes · doi:10.1055/s-2007-949591 · Cited by 12
Sone, H., Sasaki, Y., Komai, M., Toyomizu, M., Kagawa, Y., & Furukawa, Y. (2004) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2003.12.164 · Cited by 11
McGlasson, L., Best, L. C., & Brown, P. D. (2011) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2011.05.006 · Cited by 10
“In pancreatic β-cells an increase in the rate of glucose metabolism is essential for modulating the intracellular ATP/ADP concentration ratio which regulates KATP channel activity, and hence insulin secretion (Ashcroft, 2007; Detimary et al., 1998).”
BackgroundUnique features of β‐cell metabolism are lost in type 2 diabetes
Muñoz, F., Fex, M., Möritz, T., Mulder, H., & Cataldo, L. R. (2024) · Acta Physiologica · doi:10.1111/apha.14148 · Cited by 10
Brüning, D., Morsi, M., Früh, E., Scherneck, S., & Rustenbeck, I. (2022) · Endocrinology · doi:10.1210/endocr/bqac125 · Cited by 7
Physiological and Pathophysiological Control of Glucagon Secretion by Pancreatic α-Cells
Gilon, P., Cheng, R., Lai, B. K., Chae, H., & Gómez-Ruiz, A. (2014) · Springer eBooks · doi:10.1007/978-94-007-6884-0_37-2 · Cited by 8
Reciprocal regulation of insulin and plasma 5′-AMP in glucose homeostasis in mice
Xia, L., Wang, Z., Zhang, Y., Yang, X. Y., Zhan, Y., Cheng, R., Wang, S., & Zhang, J. (2014) · Journal of Endocrinology · doi:10.1530/joe-14-0501 · Cited by 8
Electrophysiology of Islet Cells
Drews, G., Krippeit‐Drews, P., & Düfer, M. (2014) · Springer eBooks · doi:10.1007/978-94-007-6686-0_5 · Cited by 7
Tamarit‐Rodriguez, J. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms25116052 · Cited by 6
Role of cAMP in Double Switch of Glucagon Secretion
Zmazek, J., Grubelnik, V., Markovič, R., & Marhl, M. (2021) · Cells · doi:10.3390/cells10040896 · Cited by 6
“The parameter value bATPase is unambiguously determined by the range of ATP concentrations (see Equation (14)), which was previously measured for pancreatic alpha cells [30].”
BackgroundPetraki, Z., Droubogiannis, S., Mylonaki, K., CHLOUVERAKIS, G. I., Plaitakis, A., & Spanaki, C. (2019) · Metabolism · doi:10.1016/j.metabol.2019.153958 · Cited by 4
Guo, Y., Liu, L., Cheng, Y., Li, H., Wan, X., Ma, J., Liu, J., Liang, W., Zhang, P., Chen, J., et al. (2023) · iScience · doi:10.1016/j.isci.2023.106451 · Cited by 3
Firdos, & Mittal, A. (2023) · ACS Omega · doi:10.1021/acsomega.3c06058 · Cited by 3
Physiological and Pathophysiological Control of Glucagon Secretion by Pancreatic α-Cells
Gilon, P., Cheng, R., Lai, B. K., Chae, H., & Gómez-Ruiz, A. (2014) · Springer eBooks · doi:10.1007/978-94-007-6686-0_37 · Cited by 3
Visual Evidence and Quantification of Interaction of Polymeric Sulfonylurea with Pancreatic Islet
Kim, S., & Bae, Y. H. (2003) · Biomacromolecules · doi:10.1021/bm025713j · Cited by 3
A Consensus Model of Glucose-Stimulated Insulin Secretion in the Pancreatic β -CellPreprint
Maheshvare, M. D., Raha, S., König, M., & Pal, D. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.03.10.532028 · Cited by 3
Putman, M. S., Hull-Meichle, R. L., Waters, S. A., Tuggle, K. L., Sommer, P., Conway, R., Borthwick, L. A., Knudsen, J. G., Dean, E. D., Stanley, S. A., et al. (2026) · Journal of Cystic Fibrosis · doi:10.1016/j.jcf.2026.03.021 · Cited by 2
Adaptation of the Secretory Machinery to Pathophysiological Conditions
Abderrahmani, A. (2008) · Springer eBooks · doi:10.1007/978-0-387-39961-4_11 · Cited by 2
Rahul, R., Stinchcombe, A. R., Joseph, J. W., & Ingalls, B. (2023) · IET Systems Biology · doi:10.1049/syb2.12077 · Cited by 1
“The resulting depolarization of the cell membrane causes an 87 influx of Ca, triggering the secretion of insulin vesicles [2].”
Henquin, J., Jonas, J., Sato, Y., DETIMARY, P., & Gilon, P. (1999) · Advances in molecular and cell biology · doi:10.1016/s1569-2558(08)60096-x · Cited by 2
Self-citationDahlgren, G. M., Nolkrantz, K., & Kennedy, R. T. (2005) · Life Sciences · doi:10.1016/j.lfs.2005.05.053 · Cited by 2
Physiological ER Stress: The Model of Insulin-Secreting Pancreatic b-Cells
Bensellam, M., Gilon, P., & Jonas, J. (2012) · Springer eBooks · doi:10.1007/978-94-007-4351-9_8 · Cited by 1
Electrophysiology of Islet Cells
Drews, G., Krippeit‐Drews, P., & Düfer, M. (2014) · Springer eBooks · doi:10.1007/978-94-007-6884-0_5-2 · Cited by 1
Glycemic Control of Apoptosis in the Pancreatic Beta Cell: Danger of Extremes?
Martens, G. A., & de Casteele, M. V. (2006) · Antioxidants and Redox Signaling · doi:10.1089/ars.2007.9.ft-16 · Cited by 1
Félix‐Martínez, G. J., Leehan, J. A., Ávila-Pozos, R., & Fernández, J. R. G. (2014) · Revista Mexicana de Ingeniería Biomédica
Zhang, X. (2015) · The University of Queensland · doi:10.14264/uql.2015.823
“5mmol/L, leading to further increase of glycolytic influx and mitochondrial oxidation [13].”
BackgroundExocytosis in Type 2 Diabetes- Functional and genetic studies of hormone secretion
Andersson, S. A. (2012) · Lund University Publications (Lund University)
Comment se parlent les cellules α et β du pancréas … ?
Dolz, M. (2008) · Médecine des Maladies Métaboliques · doi:10.1016/s1957-2557(08)71669-9
Informationsgivning vid publika förvärv
Schönbeck, J. (2005) · Lund University Publications Student Papers (Lund University)
Regulation of glucagon secretion from pancreatic alpha cells
Marinis, Y. Z. D. (2010) · Lund University Publications (Lund University)
ATP Regulates Sodium Channel Kinetics in Pancreatic Islet Beta Cells
ZouXiao, N., WuLi, Z.-Y., & Shen, L. (2013)
ATP Dynamics in Pancreatic α- and β-cells
Li, J. (2014) · KTH Publication Database DiVA (KTH Royal Institute of Technology)
Electrophysiology of Islet Cells
Drews, G., Krippeit‐Drews, P., & Düfer, M. (2013) · Springer eBooks · doi:10.1007/978-94-007-6884-0_5-1
Metadata
- Authors
- Detimary, P., Dejonghe, S., Ling, Z., Pipeleers, D., Schuit, F., & Henquin, J.-C.
- Affiliations
- UCLouvain (BE)
- Vrije Universiteit Brussel (BE)
- Corresponding author
- Henquin, Jean-Claude
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1998-12
- Volume
- 273
- Issue
- 51
- Pages
- 33905-33908
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2023149007
- Cited by
- 159
- References
- 38
- Citations by year
- 2026: 1 · 2024: 4 · 2023: 8 · 2022: 2 · 2021: 7 · 2020: 3 · 2019: 4 · 2018: 3 · 2017: 1 · 2016: 3 · 2015: 9 · 2014: 13 · 2013: 8 · 2012: 6
- FWCI
- 4.45
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DIAL (Catholic University of Leuven) (submittedVersion)
- Funders
- Fédération Wallonie-Bruxelles
- Vlaamse regering
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Pancreatic function and diabetes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes Treatment and Management
- Keywords
- adenine nucleotides, ATP/ADP ratio, pancreatic beta cells, alpha cells, glucose oxidation, insulin secretion, glucagon secretion, second messengers, human islets, duct cells
- MeSH terms
- Adenosine Diphosphate, Adenosine Triphosphate, Adult, Animals, Cells, Cultured, Glucose, Glycolysis, Humans, Islets of Langerhans, Kinetics, Male, Middle Aged, Species Specificity, Rats, Wistar, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
