The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
The Changes in Adenine Nucleotides Measured in Glucose-stimulated Rodent Islets Occur in β Cells but Not in α Cells and Are Also Observed in Human Islets
Open accessAbstract
Glucose metabolism by pancreatic beta and alpha cells is essential for stimulation of insulin secretion and inhibition of glucagon secretion. Studies using rodent islets have suggested that the ATP/ADP ratio serves as second messenger in beta cells. This study compared the effects of glucose on glucose oxidation ([U-14C]glucose) and adenine nucleotides (luminometric method) in purified rat alpha and beta cells. The rate of glucose oxidation at 1 mM glucose was higher in beta than alpha cells (4.5-fold, i.e. approximately 2-fold after normalization for cell size). It was more strongly stimulated by 10 mM glucose in beta cells (9-fold) than in alpha cells (5-fold). At 1 mM glucose, ATP levels were similar in both cell types, which corresponds to an approximately 2-fold higher concentration in alpha cells ( approximately 6.5 mM) than in beta cells ( approximately 3 mM). In beta cells, glucose dose-dependently increased ATP and decreased ADP levels, causing a rise in the ATP/ADP ratio from 2.4 to 11.6 at 1 and 10 mM, respectively. In alpha cells, glucose did not affect ATP and ADP levels, and the ATP/ADP ratio remained stable around 7.5. In human islets, the ATP/ADP ratio progressively increased between 1 and 10 mM glucose. In duct cells, which often contaminate human islet preparations, an increase in the ATP/ADP ratio sometimes occurred between 1 and 3 mM glucose. In conclusion, the present observations establish that the regulation of glucagon secretion by glucose does not involve changes in alpha cell adenine nucleotides and further support the role of the ATP/ADP ratio in the control of insulin secretion.
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“These values are in broad agreement with biochemical measurements of [ATP]i in purified rat β-cells which suggest ATP is ~2 mM under basal conditions and rises to 4 mM when glucose is increased to 10 mM (142) (see v).”
From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [142]
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Cites
A Lesson in Metabolic Regulation Inspired by the Glucokinase Glucose Sensor Paradigm
Matschinsky, F. (1996) · Diabetes · doi:10.2337/diab.45.2.223 · Cited by 721
METABOLIC COUPLING FACTORS IN PANCREATIC β-CELL SIGNAL TRANSDUCTION
Newgard, C. B., & McGarry, J. (1995) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.64.070195.003353 · Cited by 547
Gembal, M., Gilon, P., & Henquin, J. C. (1992) · Journal of Clinical Investigation · doi:10.1172/jci115714 · Cited by 494
Self-citationMechanism of glucose-induced insulin secretion.
Hedeskov, C. J. (1980) · Physiological Reviews · doi:10.1152/physrev.1980.60.2.442 · Cited by 435
Metabolic Fate of Glucose in Purified Islet Cells
Schuit, F., De Vos, A., Farfari, S., Moens, K., Pipeleers, D., Brun, T., & Prentki, M. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.30.18572 · Cited by 421
Self-citationA New in Vitro Model for the Study of Pancreatic A and B Cells*
PIPELEERS, D. G., Veld, P. I. '., Winkel, M. V. D., Maes, E., Schuit, F. C., & Gepts, W. (1985) · Endocrinology · doi:10.1210/endo-117-3-806 · Cited by 341
Self-citationInterrelationship of islet metabolism, adenosine triphosphate content and insulin release
Ashcroft, S. J. H., Weerasinghe, L., & Randle, P. J. (1973) · Biochemical Journal · doi:10.1042/bj1320223 · Cited by 301
Gembal, M., Detimary, P., Gilon, P., Gao, Z. Y., & Henquin, J. C. (1993) · Journal of Clinical Investigation · doi:10.1172/jci116308 · Cited by 260
Self-citationStimulus-secretion coupling in pancreatic β cells
Ashcroft, F. M., Proks, P., Smith, P. A., Ämmälä, C., Bokvist, K., & Rorsman, P. (1994) · Journal of Cellular Biochemistry · doi:10.1002/jcb.240550007 · Cited by 255
Endocrine Pancreas: Three-Dimensional Reconstruction Shows Two Types of Islets of Langerhans
Baetens, D., MALAISSE-LAGAE, F., Perrelet, A., & Orci, L. (1979) · Science · doi:10.1126/science.390711 · Cited by 237
Research works printed but not linked: 5
- Wollheim, 1996, Diabetes Rev., 4, 276
- Detimary, 1996, Endocrinology, 137, 4671, 10.1210/endo.137.11.8895332 · DOI
- Ronner, 1991, Diabetes, 40 (Suppl. 1), 155
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- Hellman, 1969, Diabetologia, 37 (Suppl. 2), 11
Cited by
Sorted by citations
Triggering and amplifying pathways of regulation of insulin secretion by glucose.
Henquin, J. C. (2000) · Diabetes · doi:10.2337/diabetes.49.11.1751 · Cited by 1,201
Self-citationAMP-activated protein kinase signaling in metabolic regulation
Long, Y. C. (2006) · Journal of Clinical Investigation · doi:10.1172/jci29044 · Cited by 1,125
Insulin granule dynamics in pancreatic beta cells
Rorsman, P., & Renstr�m, E. (2003) · Diabetologia · doi:10.1007/s00125-003-1153-1 · Cited by 844
“3 mmol/l, whereas Mg-ATP increases from 2 mmol/l to 4 mmol/l when glucose is increased from 1 to more than 10 mmol/l (average intracellular concentrations of ATP and ADP estimated assuming an intracellular volume of ~1 pl) [106].”
BackgroundPancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men
Rorsman, P., & Ashcroft, F. M. (2018) · Physiological Reviews · doi:10.1152/physrev.00008.2017 · Cited by 793
Mechanisms controlling pancreatic islet cell function in insulin secretion
Campbell, J. E., & Newgard, C. B. (2021) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-020-00317-7 · Cited by 682
Metadata
- Authors
- Detimary, P., Dejonghe, S., Ling, Z., Pipeleers, D., Schuit, F., & Henquin, J.-C.
- Affiliations
- UCLouvain (BE)
- Vrije Universiteit Brussel (BE)
- Corresponding author
- Henquin, Jean-Claude
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1998-12
- Volume
- 273
- Issue
- 51
- Pages
- 33905-33908
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2023149007
- Cited by
- 159
- References
- 38
- Citations by year
- 2026: 1 · 2024: 4 · 2023: 8 · 2022: 2 · 2021: 7 · 2020: 3 · 2019: 4 · 2018: 3 · 2017: 1 · 2016: 3 · 2015: 9 · 2014: 13 · 2013: 8 · 2012: 6
- FWCI
- 4.45
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DIAL (Catholic University of Leuven) (submittedVersion)
- Funders
- Fédération Wallonie-Bruxelles
- Vlaamse regering
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Pancreatic function and diabetes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes Treatment and Management
- Keywords
- adenine nucleotides, ATP/ADP ratio, pancreatic beta cells, alpha cells, glucose oxidation, insulin secretion, glucagon secretion, second messengers, human islets, duct cells
- MeSH terms
- Adenosine Diphosphate, Adenosine Triphosphate, Adult, Animals, Cells, Cultured, Glucose, Glycolysis, Humans, Islets of Langerhans, Kinetics, Male, Middle Aged, Species Specificity, Rats, Wistar, Rats
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