Fibroblast Growth Factor-16 Is a Growth Factor for Embryonic Brown Adipocytes
Open accessAbstract
In rat embryos, fibroblast growth factor (FGF)-16 is predominantly expressed in brown adipose tissue. To elucidate the role of FGF-16, we examined the expression of FGF-16 mRNA in rat embryonic brown adipose tissue at different developmental stages by Northern blotting analysis and in situ hybridization. FGF-16 mRNA was expressed abundantly in brown adipose tissue during embryonic day 17. 5, embryonic days 17.5-19.5, and thereafter at lower levels into the neonatal period. The expression profile of FGF-16 mRNA well corresponds to the proliferative profile of embryonic brown adipose tissue reported. We also examined the mitogenic activity of recombinant rat FGF-16 for primary brown adipocytes prepared from rat embryonic brown adipose tissue. FGF-16 showed significant mitogenic activity for primary brown adipocytes. The mitogenic activity was found to be exerted by binding and activating FGF receptor-4 in the brown adipose tissue. As a great induction of proliferation of rat brown adipose tissue during cold acclimation was reported, we also examined the expression of FGF-16 mRNA in the brown adipose tissue during cold acclimation by Northern blotting analysis. The expression of FGF-16 mRNA was not increased, but rather decreased. The expression profile of FGF-16 mRNA and the mitogenic activity of FGF-16 reported here indicate that FGF-16 is a unique growth factor involved in proliferation of embryonic brown adipose tissue.
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FGF-16 mRNA is expressed predominantly in brown adipose tissue during embryonic days 17.5-19.5, and thereafter at lower levels into the neonatal period.
“FGF-16 mRNA is expressed predominantly in brown adipose tissue during embryonic days 17.5–19.5, and thereafter at lower levels into the neonatal period 104.”
From Cellular bioenergetics as a target for obesity therapy (2010) · cites this paper as [104]
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- Authors
- Konishi, M., Mikami, T., Yamasaki, M., Miyake, A., & Itoh, N.
- Affiliations
- Kyoto University (JP)
- Corresponding author
- Itoh, Nobuyuki
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2000-04
- Volume
- 275
- Issue
- 16
- Pages
- 12119-12122
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2067909051
- Cited by
- 82
- References
- 19
- Citations by year
- 2025: 1 · 2024: 5 · 2023: 1 · 2022: 1 · 2021: 2 · 2020: 3 · 2019: 3 · 2017: 1 · 2016: 3 · 2015: 4 · 2014: 6 · 2013: 7 · 2012: 7
- FWCI
- 3.27
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fibroblast Growth Factor Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
- Keywords
- brown adipose tissue, embryonic development, adipocyte proliferation, mitogenic activity, FGF receptor 4, cold acclimation, rat embryo
- MeSH terms
- Uncoupling Protein 1, Animals, Adipose Tissue, Brown, Carrier Proteins, Cell Division, Cold Temperature, Fibroblast Growth Factors, Ion Channels, Membrane Proteins, RNA, Messenger, Uncoupling Agents, Rats, Wistar, In Situ Hybridization, Mitochondrial Proteins, Rats
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