Uptake of lipoproteins for axonal growth of sympathetic neurons
Open accessAbstract
Lipoproteins originating from axon and myelin breakdown in injured peripheral nerves are believed to supply cholesterol to regenerating axons. We have used compartmented cultures of rat sympathetic neurons to investigate the utilization of lipids from lipoproteins for axon elongation. Lipids and proteins from human low density lipoproteins (LDL) and high density lipoproteins (HDL) were taken up by distal axons and transported to cell bodies, whereas cell bodies/proximal axons internalized these components from only LDL, not HDL. Consistent with these observations, the impairment of axonal growth, induced by inhibition of cholesterol synthesis, was reversed when LDL or HDL were added to distal axons or when LDL, but not HDL, were added to cell bodies. LDL receptors (LDLRs) and LR7/8B (apoER2) were present in cell bodies/proximal axons and distal axons, with LDLRs being more abundant in the former. Inhibition of cholesterol biosynthesis increased LDLR expression in cell bodies/proximal axons but not distal axons. LR11 (SorLA) was restricted to cell bodies/proximal axons and was undetectable in distal axons. Neither the LDL receptor-related protein nor the HDL receptor, SR-B1, was detected in sympathetic neurons. These studies demonstrate for the first time that lipids are taken up from lipoproteins by sympathetic neurons for use in axonal regeneration.
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ApoE4-suppressed lipoprotein shuttle between astrocyte and neuron may be involved in the compromised synaptic support.
“Because lipoproteins are reported to facilitate the axonal growth of sympathetic neurons (Posse De Chaves et al., 2000), ApoE4-suppressed lipoprotein shuttle between astrocyte and neuron may be involved in the compromised synaptic support.”
From ApoE4 Impairs Neuron-Astrocyte Coupling of Fatty Acid Metabolism (2021) · cites this paper as Posse De Chaves et al., 2000
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ApoE4 Impairs Neuron-Astrocyte Coupling of Fatty Acid Metabolism
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Apolipoprotein E and cholesterol in aging and disease in the brain
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Internalization of β-Amyloid Peptide by Primary Neurons in the Absence of Apolipoprotein E
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- Authors
- Posse de Chaves, E. I., Vance, D. E., Campenot, R. B., Kiss, R. S., & Vance, J. E.
- Affiliations
- University of Alberta (CA)
- Corresponding author
- Vance, Jean E.
- ORCID
- Kiss, Robert S.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2000-06
- Volume
- 275
- Issue
- 26
- Pages
- 19883-19890
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2132983892
- Cited by
- 105
- References
- 5
- Citations by year
- 2026: 1 · 2023: 1 · 2022: 2 · 2021: 1 · 2020: 2 · 2019: 1 · 2018: 3 · 2017: 2 · 2016: 1 · 2015: 3 · 2014: 5 · 2013: 5 · 2012: 5
- FWCI
- 2.32
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Heart and Stroke Foundation of Canada
- Fondation pour la Recherche Médicale
- Medical Research Council Canada
- Medical Research Council
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
- Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Lipids, and Metabolism
- Keywords
- lipoprotein uptake, axon growth, sympathetic neurons, axon regeneration, low-density lipoprotein, high-density lipoprotein, cholesterol, LDL receptor, ApoER2, SorLA, scavenger receptor class B type I, cholesterol biosynthesis, peripheral nerve injury, lipid transport
- MeSH terms
- Animals, Animals, Newborn, Anticholesteremic Agents, Axons, Brain, Cell Division, Cells, Cultured, Electrophoresis, Polyacrylamide Gel, Humans, Lipoproteins, Lipoproteins, HDL, Lipoproteins, LDL, Liver, Membrane Proteins, Microscopy, Fluorescence, Models, Biological, Neurons, Receptors, Immunologic, Receptors, LDL, Sympathetic Nervous System, Tissue Distribution, Immunoblotting, Pravastatin, Rats, Sprague-Dawley, Receptors, Lipoprotein, CD36 Antigens, LDL-Receptor Related Proteins, Low Density Lipoprotein Receptor-Related Protein-1, Membrane Transport Proteins, Receptors, Scavenger, Scavenger Receptors, Class B, Rats
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