Cited by 78Sirtuins and Resveratrol in MedicineFungal and yeast genetics researchBiochemical and Molecular Research

Saccharomyces cerevisiae YOR071C Encodes the High Affinity Nicotinamide Riboside Transporter Nrt1

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Belenky, Peter A., Moga, Tiberiu G., Brenner, Charles·2008·Journal of Biological Chemistry

Abstract

NAD(+) is an essential coenzyme for hydride transfer enzymes and a substrate of sirtuins and other NAD(+)-consuming enzymes. Nicotinamide riboside is a recently discovered eukaryotic NAD(+) precursor converted to NAD(+) via the nicotinamide riboside kinase pathway and by nucleosidase activity and nicotinamide salvage. Nicotinamide riboside supplementation of yeast extends replicative life span on high glucose medium. The molecular basis for nicotinamide riboside uptake was unknown in any eukaryote. Here, we show that deletion of a single gene, YOR071C, abrogates nicotinamide riboside uptake without altering nicotinic acid or nicotinamide import. The gene, which is negatively regulated by Sum1, Hst1, and Rfm1, fully restores nicotinamide riboside import and utilization when resupplied to mutant yeast cells. The encoded polypeptide, Nrt1, is a predicted deca-spanning membrane protein related to the thiamine transporter, which functions as a pH-dependent facilitator with a K(m) for nicotinamide riboside of 22 microm. Nrt1-related molecules are conserved in particular fungi, suggesting a similar basis for nicotinamide riboside uptake.

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Crossref · 10.1074/jbc.c800021200

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Authors
Belenky, P. A., Moga, T. G., & Brenner, C.
Affiliations
Dartmouth–Hitchcock Medical Center (US)
Corresponding author
Brenner, Charles
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2008-03
Volume
283
Issue
13
Pages
8075-8079
ISSN
0021-9258 (ISSN-L)
OpenAlex
W2061333107
Cited by
78
References
33
Citations by year
2026: 3 · 2025: 2 · 2024: 2 · 2023: 4 · 2022: 4 · 2021: 7 · 2020: 5 · 2019: 3 · 2018: 3 · 2017: 1 · 2016: 4 · 2015: 3 · 2014: 6 · 2013: 4 · 2012: 5
FWCI
2.85
Type
journal article
Language
en
Open access
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Geriatrics and Gerontology › Sirtuins and Resveratrol in Medicine
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
Keywords
nicotinamide riboside, Saccharomyces cerevisiae, NAD+ precursors, thiamine transporter, membrane transporters, FGF4, replicative lifespan
MeSH terms
Biological Transport, Hydrogen-Ion Concentration, Kinetics, Mutation, NAD, Niacinamide, Protein Binding, Pyridinium Compounds, Saccharomyces cerevisiae, Membrane Transport Proteins, Saccharomyces cerevisiae Proteins, Nucleoside Transport Proteins

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