Saccharomyces cerevisiae YOR071C Encodes the High Affinity Nicotinamide Riboside Transporter Nrt1
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NAD(+) is an essential coenzyme for hydride transfer enzymes and a substrate of sirtuins and other NAD(+)-consuming enzymes. Nicotinamide riboside is a recently discovered eukaryotic NAD(+) precursor converted to NAD(+) via the nicotinamide riboside kinase pathway and by nucleosidase activity and nicotinamide salvage. Nicotinamide riboside supplementation of yeast extends replicative life span on high glucose medium. The molecular basis for nicotinamide riboside uptake was unknown in any eukaryote. Here, we show that deletion of a single gene, YOR071C, abrogates nicotinamide riboside uptake without altering nicotinic acid or nicotinamide import. The gene, which is negatively regulated by Sum1, Hst1, and Rfm1, fully restores nicotinamide riboside import and utilization when resupplied to mutant yeast cells. The encoded polypeptide, Nrt1, is a predicted deca-spanning membrane protein related to the thiamine transporter, which functions as a pH-dependent facilitator with a K(m) for nicotinamide riboside of 22 microm. Nrt1-related molecules are conserved in particular fungi, suggesting a similar basis for nicotinamide riboside uptake.
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Yeast cells express a dedicated NR transporter.
“Though yeast cells express a dedicated NR transporter35”
From NRK1 controls nicotinamide mononucleotide and nicotinamide riboside metabolism in mammalian cells (2016) · cites this paper as [35]
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Lee, Y., Huang, C.-C., McDaniel, M., Huang, K., Lee, L.-H., Lao, G., Karthi, D., & Lin, S.-J. (2026) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2026.111456
- Authors
- Belenky, P. A., Moga, T. G., & Brenner, C.
- Affiliations
- Dartmouth–Hitchcock Medical Center (US)
- Corresponding author
- Brenner, Charles
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2008-03
- Volume
- 283
- Issue
- 13
- Pages
- 8075-8079
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2061333107
- Cited by
- 78
- References
- 33
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 2 · 2023: 4 · 2022: 4 · 2021: 7 · 2020: 5 · 2019: 3 · 2018: 3 · 2017: 1 · 2016: 4 · 2015: 3 · 2014: 6 · 2013: 4 · 2012: 5
- FWCI
- 2.85
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Geriatrics and Gerontology › Sirtuins and Resveratrol in Medicine
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
- Keywords
- nicotinamide riboside, Saccharomyces cerevisiae, NAD+ precursors, thiamine transporter, membrane transporters, FGF4, replicative lifespan
- MeSH terms
- Biological Transport, Hydrogen-Ion Concentration, Kinetics, Mutation, NAD, Niacinamide, Protein Binding, Pyridinium Compounds, Saccharomyces cerevisiae, Membrane Transport Proteins, Saccharomyces cerevisiae Proteins, Nucleoside Transport Proteins
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