Cited by 95Sirtuins and Resveratrol in MedicineBiochemical and Molecular ResearchFolate and B Vitamins Research

Nicotinamide Riboside and Nicotinic Acid Riboside Salvage in Fungi and Mammals

Open access
Belenky, Peter, Christensen, Kathryn C., Gazzaniga, Francesca, Pletnev, Alexandre A., Brenner, Charles·2009·Journal of Biological Chemistry

Abstract

NAD+ is a co-enzyme for hydride transfer enzymes and an essential substrate of ADP-ribose transfer enzymes and sirtuins, the type III protein lysine deacetylases related to yeast Sir2. Supplementation of yeast cells with nicotinamide riboside extends replicative lifespan and increases Sir2-dependent gene silencing by virtue of increasing net NAD+ synthesis. Nicotinamide riboside elevates NAD+ levels via the nicotinamide riboside kinase pathway and by a pathway initiated by splitting the nucleoside into a nicotinamide base followed by nicotinamide salvage. Genetic evidence has established that uridine hydrolase, purine nucleoside phosphorylase, and methylthioadenosine phosphorylase are required for Nrk-independent utilization of nicotinamide riboside in yeast. Here we show that mammalian purine nucleoside phosphorylase but not methylthioadenosine phosphorylase is responsible for mammalian nicotinamide riboside kinase-independent nicotinamide riboside utilization. We demonstrate that so-called uridine hydrolase is 100-fold more active as a nicotinamide riboside hydrolase than as a uridine hydrolase and that uridine hydrolase and mammalian purine nucleoside phosphorylase cleave nicotinic acid riboside, whereas the yeast phosphorylase has little activity on nicotinic acid riboside. Finally, we show that yeast nicotinic acid riboside utilization largely depends on uridine hydrolase and nicotinamide riboside kinase and that nicotinic acid riboside bioavailability is increased by ester modification.

Integrity signals

Registry

Crossref · 10.1074/jbc.m807976200

Cites

43 of 43 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Belenky, P., Christensen, K. C., Gazzaniga, F., Pletnev, A. A., & Brenner, C.
Affiliations
Dartmouth–Hitchcock Medical Center (US)
Corresponding author
Brenner, Charles
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2009-01
Volume
284
Issue
1
Pages
158-164
ISSN
0021-9258 (ISSN-L)
OpenAlex
W2039774617
Cited by
95
References
43
Citations by year
2026: 1 · 2025: 10 · 2024: 9 · 2023: 5 · 2022: 6 · 2021: 3 · 2020: 4 · 2019: 7 · 2018: 4 · 2017: 6 · 2016: 5 · 2015: 3 · 2014: 6 · 2013: 3 · 2012: 3
FWCI
2.71
Type
journal article
Language
en
Open access
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institute of General Medical Sciences (T32GM008704)
Topics
  • Health Sciences › Medicine › Geriatrics and Gerontology › Sirtuins and Resveratrol in Medicine
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
  • Health Sciences › Medicine › Rheumatology › Folate and B Vitamins Research
Keywords
nicotinamide riboside, purine nucleoside phosphorylase, MTAP, Sir2, replicative lifespan, gene silencing, sirtuins, yeast, mammals
MeSH terms
Histone Deacetylases, Humans, NAD, Niacinamide, Purine-Nucleoside Phosphorylase, Saccharomyces cerevisiae, Phosphotransferases (Alcohol Group Acceptor), Sirtuins, Silent Information Regulator Proteins, Saccharomyces cerevisiae, Sirtuin 2

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review