Cited by 354Iron Metabolism and DisordersFungal and yeast genetics researchCellular transport and secretion

CCC1 Is a Transporter That Mediates Vacuolar Iron Storage in Yeast

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Li, Liangtao, Chen, Opal S., Ward, Diane McVey, Kaplan, Jerry·2001·Journal of Biological Chemistry

Abstract

The budding yeast Saccharomyces cerevisiae can grow for generations in the absence of exogenous iron, indicating a capacity to store intracellular iron. As cells can accumulate iron by endocytosis we studied iron metabolism in yeast that were defective in endocytosis. We demonstrated that endocytosis-defective yeast (Delta end4) can store iron in the vacuole, indicating a transfer of iron from the cytosol to the vacuole. Using several different criteria we demonstrated that CCC1 encodes a transporter that effects the accumulation of iron and Mn(2+) in vacuoles. Overexpression of CCC1, which is localized to the vacuole, lowers cytosolic iron and increases vacuolar iron content. Conversely, deletion of CCC1 results in decreased vacuolar iron content and decreased iron stores, which affect cytosolic iron levels and cell growth. Furthermore Delta ccc1 cells show increased sensitivity to external iron. The sensitivity to iron is exacerbated by ectopic expression of the iron transporter FET4. These results indicate that yeast can store iron in the vacuole and that CCC1 is involved in the transfer of iron from the cytosol to the vacuole.

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Crossref · 10.1074/jbc.m103944200

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  • A mutant lacking Ccc1 appeared in category 1, while its supposed regulator Yap5 seemed to play no role.

    “A mutant lacking Ccc1, a transporter with a possible role in vacuolar iron sequestration (26), also appeared in this category, while its supposed regulator Yap5 (27) seemed to play no role (see Dataset S1 in the supplemental material).”

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Authors
Li, L., Chen, O. S., Ward, D. M., & Kaplan, J.
Affiliations
University of Utah (US)
Corresponding author
Kaplan, Jerry
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2001-08
Volume
276
Issue
31
Pages
29515-29519
ISSN
0021-9258 (ISSN-L)
OpenAlex
W2073461422
Cited by
354
References
29
Citations by year
2026: 11 · 2025: 16 · 2024: 10 · 2023: 14 · 2022: 7 · 2021: 28 · 2020: 16 · 2019: 12 · 2018: 14 · 2017: 23 · 2016: 16 · 2015: 8 · 2014: 25 · 2013: 14 · 2012: 19
FWCI
4.99
Type
journal article
Language
en
Open access
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
National Institute of Diabetes and Digestive and Kidney Diseases (DK-5238, DK30534)
Topics
  • Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
Keywords
iron transport, Saccharomyces cerevisiae, endocytosis, manganese accumulation
MeSH terms
beta-Galactosidase, Cell Division, Ceruloplasmin, Cytosol, Fungal Proteins, Iron, Kinetics, Manganese, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Vacuoles, Gene Deletion, Genes, Reporter, DNA Primers, Cation Transport Proteins, Saccharomyces cerevisiae Proteins

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