CCC1 Is a Transporter That Mediates Vacuolar Iron Storage in Yeast
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The budding yeast Saccharomyces cerevisiae can grow for generations in the absence of exogenous iron, indicating a capacity to store intracellular iron. As cells can accumulate iron by endocytosis we studied iron metabolism in yeast that were defective in endocytosis. We demonstrated that endocytosis-defective yeast (Delta end4) can store iron in the vacuole, indicating a transfer of iron from the cytosol to the vacuole. Using several different criteria we demonstrated that CCC1 encodes a transporter that effects the accumulation of iron and Mn(2+) in vacuoles. Overexpression of CCC1, which is localized to the vacuole, lowers cytosolic iron and increases vacuolar iron content. Conversely, deletion of CCC1 results in decreased vacuolar iron content and decreased iron stores, which affect cytosolic iron levels and cell growth. Furthermore Delta ccc1 cells show increased sensitivity to external iron. The sensitivity to iron is exacerbated by ectopic expression of the iron transporter FET4. These results indicate that yeast can store iron in the vacuole and that CCC1 is involved in the transfer of iron from the cytosol to the vacuole.
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A mutant lacking Ccc1 appeared in category 1, while its supposed regulator Yap5 seemed to play no role.
“A mutant lacking Ccc1, a transporter with a possible role in vacuolar iron sequestration (26), also appeared in this category, while its supposed regulator Yap5 (27) seemed to play no role (see Dataset S1 in the supplemental material).”
From A novel hybrid iron regulation network combines features from pathogenic and nonpathogenic yeasts (2016) · cites this paper as [26]
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- Authors
- Li, L., Chen, O. S., Ward, D. M., & Kaplan, J.
- Affiliations
- University of Utah (US)
- Corresponding author
- Kaplan, Jerry
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2001-08
- Volume
- 276
- Issue
- 31
- Pages
- 29515-29519
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2073461422
- Cited by
- 354
- References
- 29
- Citations by year
- 2026: 11 · 2025: 16 · 2024: 10 · 2023: 14 · 2022: 7 · 2021: 28 · 2020: 16 · 2019: 12 · 2018: 14 · 2017: 23 · 2016: 16 · 2015: 8 · 2014: 25 · 2013: 14 · 2012: 19
- FWCI
- 4.99
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Institute of Diabetes and Digestive and Kidney Diseases (DK-5238, DK30534)
- Topics
- Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Keywords
- iron transport, Saccharomyces cerevisiae, endocytosis, manganese accumulation
- MeSH terms
- beta-Galactosidase, Cell Division, Ceruloplasmin, Cytosol, Fungal Proteins, Iron, Kinetics, Manganese, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Vacuoles, Gene Deletion, Genes, Reporter, DNA Primers, Cation Transport Proteins, Saccharomyces cerevisiae Proteins
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