The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Analyses of p53 Target Genes in the Human Genome by Bioinformatic and Microarray Approaches
Open accessAbstract
The completion of the human genome sequence (International Human Genome Sequence Consortium (2001) Nature 409, 860-921; Venter, J. C., et al. (2001) Science 291, 1304-1351) allows for new ways to analyze global cellular regulatory mechanisms. Here we present a strategy to identify genes regulated by specific transcription factors in the human genome, and apply it to p53. We first collected promoters or introns of all genes available using two methods: GenBank(TM) annotation and a computationally derived transcript map. 4,852 genes analyzed in this way contained at least one p53 consensus binding sequence. Of 13 genes randomly selected for mRNA analysis, 11 were shown to respond to p53 expression. Five promoters were analyzed by chromatin immunoprecipitation, which revealed that all were bound by p53 in vivo. We then analyzed 33,615 unique human genes on cDNA microarrays, identifying 1,501 genes that respond to p53 expression. A parameter was derived that demonstrates that in silico prediction greatly enriches for genes that are activated and repressed by p53 and assists us to suggest other signaling pathways that may be connected to p53. The methods shown here illustrate a novel approach to analysis of global gene regulatory network through the integration of human genomic sequence information and genome-wide gene expression analysis.
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Vogelstein, B., Lane, D., & Levine, A. J. (2000) · Nature · doi:10.1038/35042675 · Cited by 6,607
Tumor suppressor p53 is a direct transcriptional activator of the human bax gene
Toshiyuki, M., & Reed, J. C. (1995) · Cell · doi:10.1016/0092-8674(95)90412-3 · Cited by 4,063
Definition of a consensus binding site for p53
El-Deiry, W. S., Kern, S. E., Pietenpol, J. A., Kinzler, K. W., & Vogelstein, B. (1992) · Nature Genetics · doi:10.1038/ng0492-45 · Cited by 1,985
p53 Suppresses the Activation of the Bcl-2 Promoter by the Brn-3a POU Family Transcription Factor
Budhram-Mahadeo, V., Morris, P. J., Smith, M. D., Midgley, C. A., Boxer, L. M., & Latchman, D. S. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.21.15237 · Cited by 144
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Cited by
Transcriptional control of human p53-regulated genes
Riley, T., Sontag, E., Chen, P., & Levine, A. (2008) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2395 · Cited by 1,921
The transcription factor p53: Not a repressor, solely an activator.
Fischer, M., Steiner, L., & Engeland, K. (2014) · Cell Cycle · doi:10.4161/15384101.2014.949083 · Cited by 153
Jackson, J. G., & Pereira-Smith, O. M. (2006) · Molecular and Cellular Biology · doi:10.1128/mcb.26.7.2501-2510.2006 · Cited by 108
High-Affinity Binding of Tumor-Suppressor Protein p53 and HMGB1 to Hemicatenated DNA Loops
Štros, M., Muselíková-Polanská, E., Pospíšilová, Š., & Strauss, F. (2004) · Biochemistry · doi:10.1021/bi049928k · Cited by 81
Transcriptional networks and cellular senescence in human mammary fibroblasts.
Hardy, K., Mansfield, L., Mackay, A., Benvenuti, S., Ismail, S., Arora, P., O'Hare, M. J., & Jat, P. S. (2005) · Molecular Biology of the Cell · doi:10.1091/mbc.e04-05-0392 · Cited by 71
Metadata
- Authors
- Wang, L., Wu, Q., Qiu, P., Mirza, A., McGuirk, M., Kirschmeier, P., Greene, J. R., Wang, Y., Pickett, C. B., & Liu, S.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2001-11
- Volume
- 276
- Issue
- 47
- Pages
- 43604-43610
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1976842438
- Cited by
- 166
- References
- 5
- FWCI
- 6.92
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Wang, L., Wu, Q., Qiu, P., Mirza, A., McGuirk, M., Kirschmeier, P., Greene, J. R., Wang, Y., Pickett, C. B., & Liu, S. (2001). Analyses of p53 Target Genes in the Human Genome by Bioinformatic and Microarray Approaches. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.m106570200
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