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Selective Survival Rescue in 15-Lipoxygenase-1-deficient Retinal Pigment Epithelial Cells by the Novel Docosahexaenoic Acid-derived Mediator, Neuroprotectin D1
Open accessAbstract
The integrity of the retinal pigment epithelial (RPE) cell is essential for the survival of rod and cone photoreceptor cells. Several stressors, including reactive oxygen species, trigger apoptotic damage in RPE cells preceded by an anti-inflammatory, pro-survival response, the formation of neuroprotectin D1 (NPD1), an oxygenation product derived from the essential omega-3 fatty acid family member docosahexaenoic acid. To define the ability of NPD1 and other endogenous novel lipid mediators in cell survival, we generated a stable knockdown human RPE (ARPE-19) cell line using short hairpin RNA to target 15-lipoxygenase-1. The 15-lipoxygenase-1-deficient cells exhibited 30% of the protein expression, and 15-lipoxygenase-2 remained unchanged, as compared with an ARPE-19 cell line control established using nonspecific short hairpin RNA transfected cells. NPD1 synthesis was stimulated by tumor necrosis factor alpha/H2O2-mediated oxidative stress in nonspecific cells (controls), whereas in silenced cells, negligible amounts of NPD1, 12(S)- and 15(S)-hydroxyeicosatetraenoic acid, and lipoxin A4 were found under these conditions. Neither control nor the deficient cells showed an increase in 15-lipoxygenase-1 protein content after 16 h of oxidative stress, suggesting that the increased activity of 15-lipoxygenase-1 is due to activation of pre-existing proteins. 15-Lipoxygenase-silenced cells also displayed an exacerbated sensitivity to oxidative stress-induced apoptosis when compared with the control cells. NPD1 selectively and potently rescued 15-lipoxygenase-silenced cells from oxidative stress-induced apoptosis. These results demonstrate that 15-lipoxygenase-1 is activated by oxidative stress in ARPE-19 cells and that NPD1 is part of an early survival signaling in RPE cells.
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“sAPPα may activate NPD1 biosynthetic enzymes PLA2 and/or a 15-LOX– like enzyme integral to NPD1 biosynthesis (Calandria et al., 2009; Marcheselli et al., 2003).”
From Docosanoids and elovanoids from omega-3 fatty acids are pro-homeostatic modulators of inflammatory responses, cell damage and neuroprotection (2018) · cites this paper as Calandria et al., 2009
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Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
The Retinal Pigment Epithelium in Visual Function
Strauss, O. (2005) · Physiological Reviews · doi:10.1152/physrev.00021.2004 · Cited by 2,828
Resolvin E1 and protectin D1 activate inflammation-resolution programmes
Schwab, J. M., Chiang, N., Arita, M., & Serhan, C. N. (2007) · Nature · doi:10.1038/nature05877 · Cited by 1,219
Serhan, C. N. (2006) · Annual Review of Immunology · doi:10.1146/annurev.immunol.25.022106.141647 · Cited by 1,020
Lukiw, W. J. (2005) · Journal of Clinical Investigation · doi:10.1172/jci25420 · Cited by 897
Marcheselli, V. L., Hong, S., Lukiw, W. J., Tian, X. H., Gronert, K., Musto, A., Hardy, M., Gimenez, J. M., Chiang, N., Serhan, C. N., et al. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m305841200 · Cited by 802
Self-citationMukherjee, P. K., Marcheselli, V. L., Serhan, C. N., & Bazan, N. G. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0402531101 · Cited by 753
Osakada, F., Ikeda, H. O., Mandai, M., Wataya, T., Watanabe, K., Yoshimura, N., Akaike, A., Sasai, Y., & Takahashi, M. (2008) · Nature Biotechnology · doi:10.1038/nbt1384 · Cited by 629
The retinal pigment epithelium: a versatile partner in vision
Bok, D. (1993) · Journal of Cell Science · doi:10.1242/jcs.1993.supplement_17.27 · Cited by 593
Membrane docosahexaenoate is supplied to the developing brain and retina by the liver.
Scott, B. L., & Bazan, N. G. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.8.2903 · Cited by 448
Discovery of a second 15 S -lipoxygenase in humans
BRASH, A. R., Boeglin, W. E., & Chang, M. S. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.12.6148 · Cited by 402
PEDF: a multifaceted neurotrophic factor
Tombran‐Tink, J., & Barnstable, C. J. (2003) · Nature reviews. Neuroscience · doi:10.1038/nrn1176 · Cited by 369
Cell survival matters: docosahexaenoic acid signaling, neuroprotection and photoreceptors
Bazan, N. G. (2006) · Trends in Neurosciences · doi:10.1016/j.tins.2006.03.005 · Cited by 353
Gronert, K., Maheshwari, N., Khan, N. A., Hassan, I., Dunn, M. W., & Schwartzman, M. L. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m410638200 · Cited by 312
Ariel, A., Li, P., Wang, W., Tang, W.-X., Fredman, G., Hong, S., Gotlinger, K. H., & Serhan, C. N. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m509796200 · Cited by 254
Macular degeneration: recent advances and therapeutic opportunities
Rattner, A., & Nathans, J. (2006) · Nature reviews. Neuroscience · doi:10.1038/nrn2007 · Cited by 233
Circadian nature of rod outer segment disc shedding in the rat.
LaVail, M. M. (1980) · PubMed · Cited by 199
Neurotrophins enhance retinal pigment epithelial cell survival through neuroprotectin D1 signaling
Mukherjee, P. K., Marcheselli, V. L., Barreiro, S., Hu, J., Bok, D., & Bazan, N. G. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0705949104 · Cited by 170
Brinckmann, R., Schnurr, K., Heydeck, D., Rosenbach, T., Kolde, G., & Kühn, H. (1998) · Blood · doi:10.1182/blood.v91.1.64 · Cited by 146
Bazan, N. G. (2007) · Investigative Ophthalmology & Visual Science · doi:10.1167/iovs.07-0918 · Cited by 119
Mukherjee, P. K., Marcheselli, V. L., Vaccari, J. C. D. R., Gordon, W. C., Jackson, F. R., & Bazan, N. G. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0705963104 · Cited by 110
Defining the Arachidonic Acid Binding Site of Human 15-Lipoxygenase
Gan, Q.-F., Browner, M. F., Sloane, D. L., & Sigal, E. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.41.25412 · Cited by 93
Retinal light damage in rats with altered levels of rod outer segment docosahexaenoate.
Organisciak, D. T., Darrow, R. M., Jiang, Y.-L., & Blanks, J. C. (1996) · PubMed · Cited by 83
Investigations into Calcium-dependent Membrane Association of 15-Lipoxygenase-1
Walther, M., Wiesner, R., & Kühn, H. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m309564200 · Cited by 83
Engelhardt, M., Bogdahn, U., & Aigner, L. (2005) · Brain Research · doi:10.1016/j.brainres.2005.01.075 · Cited by 77
Aleem, A. M., Jankun, J., Dignam, J. D., Walther, M., Kühn, H., Svergun, D. I., & Skrzypczak‐Jankun, E. (2007) · Journal of Molecular Biology · doi:10.1016/j.jmb.2007.11.086 · Cited by 68
Chen, B., Tsui, S., Boeglin, W. E., Douglas, R. S., BRASH, A. R., & Smith, T. J. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m603484200 · Cited by 48
McKay, B. S., Goodman, B. A., Falk, T., & Sherman, S. J. (2006) · Experimental Neurology · doi:10.1016/j.expneurol.2006.04.016 · Cited by 40
Detection and Subcellular Localization of Two 15S-Lipoxygenases in Human Cornea
Chang, M. S., Schneider, C. G., Roberts, R. L., Shappell, S. B., Haselton, F. R., Boeglin, W. E., & BRASH, A. R. (2005) · Investigative Ophthalmology & Visual Science · doi:10.1167/iovs.04-1166 · Cited by 37
Kühn, H., Thiele, B. J., Ostareck‐Lederer, A., Stender, H., Suzuki, H., Yoshimoto, T., & Yamamoto, S. (1993) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(93)90268-e · Cited by 31
Liang, L., Yan, R., Li, X., Chimento, M. F., & Wang, S. (2008) · Investigative Ophthalmology & Visual Science · doi:10.1167/iovs.07-1380 · Cited by 15
Lanning, J. L., Wallace, J. S., Zhang, D., Diwakar, G., Jiao, Z., & Hornyak, T. J. (2005) · Journal of Investigative Dermatology · doi:10.1111/j.0022-202x.2005.23819.x · Cited by 13
When Pigment Cells Turn Into Neurons
Bharti, K., & Arnheiter, H. (2005) · Journal of Investigative Dermatology · doi:10.1111/j.0022-202x.2005.23876.x · Cited by 3
Research works printed but not linked: 2
- LaVail, 1980, Invest. Ophthalmol. Vis. Sci., 19, 407
- Organisciak, 1996, Invest. Ophthalmol. Vis. Sci., 37, 2243
Cited by
Sorted by citations
Resolvins and protectins in inflammation resolution
Serhan, C. N., & Petasis, N. A. (2011) · Chemical Reviews · doi:10.1021/cr100396c · Cited by 996
Self-citationSerhan, C. N., Dalli, J., Colas, R. A., Winkler, J. W., & Chiang, N. (2015) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2014.08.006 · Cited by 492
Specialized pro-resolving lipid mediators in the inflammatory response: An update
Bannenberg, G., & Serhan, C. N. (2010) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2010.08.002 · Cited by 464
Bazan, N. G., Molina, M. F., & Gordon, W. C. (2011) · Annual Review of Nutrition · doi:10.1146/annurev.nutr.012809.104635 · Cited by 441
Self-citationWeylandt, K. H., Chiu, C., Gomolka, B., Waechter, S. F., & Wiedenmann, B. (2012) · Prostaglandins & Other Lipid Mediators · doi:10.1016/j.prostaglandins.2012.01.005 · Cited by 273
Metadata
- Authors
- Calandria, J. M., Marcheselli, V. L., Mukherjee, P. K., Uddin, J., Winkler, J. W., Petasis, N. A., & Bazan, N. G.
- Affiliations
- Louisiana State University (US)
- University of Southern California (US)
- Corresponding author
- Bazan, Nicolas G.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2009-06
- Volume
- 284
- Issue
- 26
- Pages
- 17877-17882
- ISSN
- 0021-9258 (ISSN-L)
- PMC
- PMC2719426
- OpenAlex
- W2022004671
- Cited by
- 104
- References
- 33
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 7 · 2023: 1 · 2022: 5 · 2021: 5 · 2020: 3 · 2019: 4 · 2018: 6 · 2017: 9 · 2016: 5 · 2015: 9 · 2014: 2 · 2013: 10 · 2012: 9
- FWCI
- 10.97
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (EY0-05121, RR016816)
- National Eye Institute (R01EY005121)
- National Center for Research Resources (P20RR016816)
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
- Health Sciences › Medicine › Ophthalmology › Retinal Diseases and Treatments
- Health Sciences › Medicine › Biochemistry (medical) › Antioxidant Activity and Oxidative Stress
- Keywords
- neuroprotectin D1, 15-lipoxygenase-1, retinal pigment epithelial cells, docosahexaenoic acid, oxidative stress, apoptosis, lipoxin A4, hydroxyeicosatetraenoic acids, photoreceptor survival, ARPE-19 cells
- MeSH terms
- Arachidonate 15-Lipoxygenase, Docosahexaenoic Acids, Humans, Hydrogen Peroxide, Immunoenzyme Techniques, Pigment Epithelium of Eye, Retina, Tumor Necrosis Factor-alpha, Blotting, Western, Lipoxygenase Inhibitors, Oxidants, Apoptosis, Oxidative Stress, RNA, Small Interfering, Lipoxins
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