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AMPK β1 Deletion Reduces Appetite, Preventing Obesity and Hepatic Insulin Resistance
Open accessAbstract
The AMP-activated protein kinase (AMPK) is an alphabetagamma heterotrimer that regulates appetite and fuel metabolism. We have generated AMPK beta1(-/-) mice on a C57Bl/6 background that are viable, fertile, survived greater than 2 years, and display no visible brain developmental defects. These mice have a 90% reduction in hepatic AMPK activity due to loss of the catalytic alpha subunits, with modest reductions of activity detected in the hypothalamus and white adipose tissue and no change in skeletal muscle or heart. On a low fat or an obesity-inducing high fat diet, beta1(-/-) mice had reduced food intake, reduced adiposity, and reduced total body mass. Metabolic rate, physical activity, adipose tissue lipolysis, and lipogenesis were similar to wild type littermates. The reduced appetite and body mass of beta1(-/-) mice were associated with protection from high fat diet-induced hyperinsulinemia, hepatic steatosis, and insulin resistance. We demonstrate that the loss of beta1 reduces food intake and protects against the deleterious effects of an obesity-inducing diet.
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“AMPK β1 KO mice have reduced AMPK activity in liver but not skeletal muscle, are hyperphagic and as a result have lower body weight, adiposity and hepatic lipid accumulation, and are protected from high-fat diet induced insulin resistance [27].”
From AMPK and Exercise: Glucose Uptake and Insulin Sensitivity (2013) · cites this paper as [27]
In the citing paper
“AMPK β1 KO mice have reduced AMPK activity in liver but not skeletal muscle, are hyperphagic and as a result have lower body weight, adiposity and hepatic lipid accumulation, and are protected from high-fat diet induced insulin resistance [27].”
From AMPK and Exercise: Glucose Uptake and Insulin Sensitivity (2013) · cites this paper as [27]
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Metadata
- Authors
- Dzamko, N., van Denderen, B. J. W., Hevener, A. L., Jørgensen, S. B., Honeyman, J., Galic, S., Chen, Z.-P., Watt, M. J., Campbell, D. J., Steinberg, G. R., & Kemp, B. E.
- Affiliations
- The University of Melbourne (AU)
- University of California, Los Angeles (US)
- University of Copenhagen (DK)
- Monash University (AU)
- Corresponding author
- Steinberg, Gregory R.
- Kemp, Bruce E.
- ORCID
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2010-01
- Volume
- 285
- Issue
- 1
- Pages
- 115-122
- ISSN
- 0021-9258 (ISSN-L)
- PMC
- PMC2804155
- OpenAlex
- W2028308875
- Cited by
- 175
- References
- 43
- Citations by year
- 2026: 3 · 2025: 5 · 2024: 6 · 2023: 7 · 2022: 12 · 2021: 11 · 2020: 13 · 2019: 8 · 2018: 10 · 2017: 9 · 2016: 16 · 2015: 9 · 2014: 14 · 2013: 15 · 2012: 15
- FWCI
- 4.70
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Regulation of Appetite and Obesity
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adipokines, Inflammation, and Metabolic Diseases
- Keywords
- AMPK, appetite regulation, dietary intake, obesity, hepatic insulin resistance, hyperinsulinemia, hepatic steatosis, high-fat diet, knockout mice, adipose tissue lipolysis, lipogenesis, metabolic rate
- MeSH terms
- AMP-Activated Protein Kinase Kinases, Animals, Appetite, Body Weight, Carbon Dioxide, Darkness, Dietary Fats, Fasting, Feeding Behavior, Gene Expression Regulation, Gluconeogenesis, Insulin, Insulin Resistance, Liver, Mice, Inbred C57BL, Obesity, Organ Specificity, Oxidation-Reduction, Oxygen, Protein Kinases, Respiration, Gene Deletion, Protein Subunits, Hepatocytes, Mice
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