The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.
Rapamycin and Glucose-Target of Rapamycin (TOR) Protein Signaling in Plants
Open accessAbstract
Target of rapamycin (TOR) kinase is an evolutionarily conserved master regulator that integrates energy, nutrients, growth factors, and stress signals to promote survival and growth in all eukaryotes. The reported land plant resistance to rapamycin and the embryo lethality of the Arabidopsis tor mutants have hindered functional dissection of TOR signaling in plants. We developed sensitive cellular and seedling assays to monitor endogenous Arabidopsis TOR activity based on its conserved S6 kinase (S6K) phosphorylation. Surprisingly, rapamycin effectively inhibits Arabidopsis TOR-S6K1 signaling and retards glucose-mediated root and leaf growth, mimicking estradiol-inducible tor mutants. Rapamycin inhibition is relieved in transgenic plants deficient in Arabidopsis FK506-binding protein 12 (FKP12), whereas FKP12 overexpression dramatically enhances rapamycin sensitivity. The role of Arabidopsis FKP12 is highly specific as overexpression of seven closely related FKP proteins fails to increase rapamycin sensitivity. Rapamycin exerts TOR inhibition by inducing direct interaction between the TOR-FRB (FKP-rapamycin binding) domain and FKP12 in plant cells. We suggest that variable endogenous FKP12 protein levels may underlie the molecular explanation for longstanding enigmatic observations on inconsistent rapamycin resistance in plants and in various mammalian cell lines or diverse animal cell types. Integrative analyses with rapamycin and conditional tor and fkp12 mutants also reveal a central role of glucose-TOR signaling in root hair formation. Our studies demonstrate the power of chemical genetic approaches in the discovery of previously unknown and pivotal functions of glucose-TOR signaling in governing the growth of cotyledons, true leaves, petioles, and primary and secondary roots and root hairs.
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Cites
TOR signaling in growth and metabolism
Wullschleger, S., Loewith, R., & Hall, M. N. (2006) · Cell · doi:10.1016/j.cell.2006.01.016 · Cited by 5,787
Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis
Yoo, S.-D., Cho, Y.-H., & Sheen, J. (2007) · Nature Protocols · doi:10.1038/nprot.2007.199 · Cited by 5,083
Self-citationmTOR: from growth signal integration to cancer, diabetes and ageing
Zoncu, R., Efeyan, A., & Sabatini, D. M. (2011) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3025 · Cited by 3,941
MAP kinase signalling cascade in Arabidopsis innate immunity
Asai, T., Tena, G., Plotnikova, J., Willmann, M. R., Chiu, W.-L., Gomez-Gomez, L., Boller, T., Ausubel, F. M., & Sheen, J. (2002) · Nature · doi:10.1038/415977a · Cited by 2,726
Self-citationSUGAR SENSING AND SIGNALING IN PLANTS: Conserved and Novel Mechanisms
Rolland, F., Baena-Gonzalez, E., & Sheen, J. (2006) · Annual Review of Plant Biology · doi:10.1146/annurev.arplant.57.032905.105441 · Cited by 2,328
Self-citationRheb GTPase is a direct target of TSC2 GAP activity and regulates mTOR signaling
Inoki, K., Li, Y., Xu, T., & Guan, K.-L. (2003) · Genes & Development · doi:10.1101/gad.1110003 · Cited by 1,911
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Construct design for efficient, effective and high‐throughput gene silencing in plants
Wesley, S. V., Helliwell, C. A., Smith, N. A., Wang, M., Rouse, D. T., Liu, Q., Gooding, P. S., Singh, S. P., Abbott, D., Stoutjesdijk, P. A., et al. (2001) · The Plant Journal · doi:10.1046/j.1365-313x.2001.01105.x · Cited by 1,532
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Role of the Arabidopsis Glucose Sensor HXK1 in Nutrient, Light, and Hormonal Signaling
Moore, B., Zhou, L., Rolland, F., Hall, Q., Cheng, W.-H., Liu, Y.-X., Hwang, I., Jones, T., & Sheen, J. (2003) · Science · doi:10.1126/science.1080585 · Cited by 1,181
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Cited by
Glucose–TOR signalling reprograms the transcriptome and activates meristems
Xiong, Y., McCormack, M., Li, L., Hall, Q., Xiang, C., & Sheen, J. (2013) · Nature · doi:10.1038/nature12030 · Cited by 818
Self-citationNukarinen, E., Nägele, T., Pedrotti, L., Wurzinger, B., Mair, A., Landgraf, R., Börnke, F., Hanson, J., Teige, M., Baena-Gonzalez, E., et al. (2016) · Scientific Reports · doi:10.1038/srep31697 · Cited by 334
Differential TOR activation and cell proliferation in Arabidopsis root and shoot apexes
Li, X., Cai, W., Liu, Y., Li, H., Fu, L., Liu, Z., Xu, L., Liu, H., Xu, T., & Xiong, Y. (2017) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1618782114 · Cited by 316
Self-citationDynamic and diverse sugar signaling
Li, L., & Sheen, J. (2016) · Current Opinion in Plant Biology · doi:10.1016/j.pbi.2016.06.018 · Cited by 304
Self-citationBroeckx, T., Hulsmans, S., & Rolland, F. (2016) · Journal of Experimental Botany · doi:10.1093/jxb/erw416 · Cited by 267
Metadata
- Authors
- Xiong, Y., & Sheen, J.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2012-01
- Volume
- 287
- Issue
- 4
- Pages
- 2836-2842
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2168925623
- Cited by
- 299
- References
- 35
- FWCI
- 4.23
- Type
- journal article
- Language
- en
- Open access
- hybrid
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