Zinc Finger Protein Prz1 Regulates Ca2+ but Not Cl− Homeostasis in Fission Yeast
Open accessAbstract
Calcineurin is an important mediator that connects the Ca(2+)-dependent signaling to various cellular responses in a wide variety of cell types and organisms. In budding yeast, activated calcineurin exerts its function mainly by regulating the Crz1p/Tcn1 transcription factor. Here, we cloned the fission yeast prz1(+) gene, which encodes a zinc finger transcription factor highly homologous to Crz1/Tcn1. Similar to the results in budding yeast, calcineurin dephosphorylated Prz1 and resulted in the trans-location of Prz1 from the cytoplasm to the nucleus. Prz1 expression was stimulated by high extracellular Ca(2+) in a calcineurin-dependent fashion. However, unlike in budding yeast, the prz1-null cells did not show any phenotype similar to those previously reported in calcineurin deletion such as aberrant cell morphology, mating defect, or hypersensitivity to Cl(-). Instead, the prz1-null cells showed hypersensitivity to Ca(2+), consistent with a dramatic decrease in transcription of Pmc1 Ca(2+) pump. Interestingly, overexpression of Prz1 did not suppress the Cl(-) hypersensitivity of calcineurin deletion, and overexpression of Pmp1 MAPK phosphatase suppressed the Cl(-) hypersensitivity of calcineurin deletion but not the Ca(2+) hypersensitivity of prz1 deletion. In addition, mutations in the its2(+)/cps1(+), its8(+), and its10(+)/cdc7(+) genes that showed synthetic lethal genetic interaction with calcineurin deletion did not exhibit synthetic lethality with the prz1 deletion. Our results suggest that calcineurin activates at least two distinct signaling branches, i.e. the Prz1-dependent transcriptional regulation and an unknown mechanism, which functions antagonistically with the Pmk1 MAPK pathway.
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Defective prz1 and crz1 yeasts exhibit hypersensitivity to calcium ions, consistent with decreased expression of the Pmc1 Ca2+-pump.
“Like CRZ1, Prz1+ gene is not required for cell growth in standard conditions but defective prz1and crz1-yeasts exhibit hypersensitivity to calcium ions, consistent with a decreased expression of the Pmc1 Ca 2+ -pump.”
From Functional characterization of the Candida albicans CRZ1 gene encoding a calcineurin-regulated transcription factor (2005) · cites this paper as Hirayama et al. 2003
In the model and fission yeasts, the high-calcium hypersensitivity phenotype has been associated with decreased expression of the calcineurin-regulated PMC1 gene encoding the vacuolar calcium pump.
“In the model and fission yeasts, this phenotype has been associated to a decreased expression of the calcineurin-regulated PMC1 gene encoding the vacuolar calcium pump (Matheos et al. 1997;Hirayama et al. 2003).”
From Functional characterization of the Candida albicans CRZ1 gene encoding a calcineurin-regulated transcription factor (2005) · cites this paper as Hirayama et al. 2003
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- Authors
- Hirayama, S., Sugiura, R., Lu, Y., Maeda, T., Kawagishi, K., Yokoyama, M., Tohda, H., Giga-Hama, Y., Shuntoh, H., & Kuno, T.
- Affiliations
- Kobe University (JP)
- Asahi Glass (Japan) (JP)
- Corresponding author
- Kuno, Takayoshi
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2003-05
- Volume
- 278
- Issue
- 20
- Pages
- 18078-18084
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2074971567
- Cited by
- 87
- References
- 45
- Citations by year
- 2025: 5 · 2024: 2 · 2023: 1 · 2022: 6 · 2021: 7 · 2020: 5 · 2019: 4 · 2018: 4 · 2017: 3 · 2016: 3 · 2015: 3 · 2014: 5 · 2013: 4 · 2012: 4
- FWCI
- 1.38
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Cancer Research UK
- Ministry of Education, Culture, Sports, Science and Technology
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Signaling Pathways in Disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- zinc finger transcription factor, calcineurin, Schizosaccharomyces pombe, transcriptional regulation, synthetic lethality, nuclear translocation
- MeSH terms
- Amino Acid Sequence, Calcium, Carrier Proteins, Cell Nucleus, Chlorides, Cloning, Molecular, Cytoplasm, DNA-Binding Proteins, Immunosuppressive Agents, Luminescent Proteins, Microscopy, Fluorescence, Models, Biological, Molecular Sequence Data, Phosphorylation, RNA, Messenger, Schizosaccharomyces, Temperature, Transcription Factors, Immunoblotting, Signal Transduction, Trans-Activators, Gene Deletion, Calcineurin, MAP Kinase Signaling System, Protein Transport, Saccharomyces cerevisiae Proteins, Schizosaccharomyces pombe Proteins, Green Fluorescent Proteins
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