The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
Standard Isolation of Primary Adipose Cells from Mouse Epididymal Fat Pads Induces Inflammatory Mediators and Down-regulates Adipocyte Genes
Open accessAbstract
Isolation and subsequent in vitro culture of primary adipose cells are associated with down-regulation of GLUT4 mRNA and simultaneous induction of GLUT1 gene expression. Progressive loss of insulin-responsive GLUT4 contributes to the decrease in insulin-mediated glucose uptake in these cells when cultured in vitro. The mechanisms underlying these alterations are unknown. Here, we report that the standard procedure for isolating primary adipose cells from mouse adipose tissue triggers induction of many genes encoding inflammatory mediators including TNF-alpha, interleukin (IL)-1 alpha, IL-6, multiple chemokines, cell adhesion molecules, acute-phase proteins, type I IL-1 receptor, and multiple transcription factors implicated in the cellular inflammatory response. Secretion of TNF-alpha protein was also significantly induced during the 2-h collagenase digestion of adipose tissue. Isolated primary adipose cells exhibit dramatic changes in expression of multiple mRNAs that are characteristic of TNF-alpha-treated 3T3-L1 adipocytes including down-regulation of many genes important for insulin action and triglyceride synthesis. Addition of TNF-alpha to primary adipose cells in culture did not change the kinetics or the extent of the repression of adipose cell-abundant genes. Moreover, TNF-alpha-neutralizing antibody failed to block the changes in gene transcription in isolated primary adipose cells. Also, the standard isolation procedure induced the expression of NF-kappa B family members and their target genes in primary adipose cells prepared from TNF-alpha-/- mice to the same extent as in cells isolated from wild-type mice and resulted in almost identical changes in global gene expression when these cells were cultured in vitro. Thus, these data suggest that the standard isolation procedure-triggered reprogramming of gene expression in primary adipose cells that results in decreased insulin sensitivity does not require TNF-alpha, at least in this in vitro model system, but may be dependent on other inflammatory cytokines produced by these cells.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“It cannot be excluded that this enzymatic digestion technique may have a potential influence on the expression profiles of separated cells, as noted previously in mouse studies [65].”
From Adipose tissue transcriptomic signature highlights the pathological relevance of extracellular matrix in human obesity (2008) · cites this paper as [65]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Kohonen, T. (1990) · Proceedings of the IEEE · doi:10.1109/5.58325 · Cited by 8,414
Mammalian MAP kinase signalling cascades
Chang, L., & Karin, M. (2001) · Nature · doi:10.1038/35065000 · Cited by 5,118
Tamayo, P., Slonim, D. K., Mesirov, J. P., Zhu, Q., Kitareewan, S., Dmitrovsky, E., Lander, E. S., & Golub, T. R. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.6.2907 · Cited by 2,924
Pathogenesis of NIDDM. A balanced overview.
DeFronzo, R. A., Bonadonna, R. C., & Ferrannini, E. (1992) · Diabetes Care · doi:10.2337/diacare.15.3.318 · Cited by 2,085
Cho, H., Mu, J., Kim, J. K., Thorvaldsen, J. L., Chu, Q., Crenshaw, E. B., Kaestner, K. H., Bartolomei, M. S., Shulman, G. I., & Birnbaum, M. J. (2001) · Science · doi:10.1126/science.292.5522.1728 · Cited by 1,860
Physiological role of adipose tissue: white adipose tissue as an endocrine and secretory organ
Trayhurn, P., & Beattie, J. H. (2001) · Proceedings of The Nutrition Society · doi:10.1079/pns200194 · Cited by 1,292
Thiazolidinediones in the Treatment of Insulin Resistance and Type II Diabetes
Saltiel, A. R., & Olefsky, J. M. (1996) · Diabetes · doi:10.2337/diab.45.12.1661 · Cited by 1,050
Matsusaka, T., Fujikawa, K., Nishio, Y., Mukaida, N., Matsushima, K., Kishimoto, T., & Akira, S. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.21.10193 · Cited by 959
Dunne, A., & O'Neill, L. A. J. (2003) · Science Signaling · doi:10.1126/stke.2003.171.re3 · Cited by 761
Potential Role of TNF-α in the Pathogenesis of Insulin Resistance and Type 2 Diabetes
Moller, D. E. (2000) · Trends in Endocrinology and Metabolism · doi:10.1016/s1043-2760(00)00272-1 · Cited by 730
Flagged first, then by citations
Cited by
Sorted by citations
Kim, H. J., Park, S. Y., Choi, Y. J., Han, S. J., Lee, K. W., & Kim, D. J. (2013) · Hormone Research in Paediatrics · doi:10.1159/000348848 · Cited by 3,061
Fain, J. N., Madan, A. K., Hiler, M. L., Cheema, P. S., & Bahouth, S. W. (2004) · Endocrinology · doi:10.1210/en.2003-1336 · Cited by 1,619
Molecular mechanisms of cancer development in obesity
Khandekar, M. J., Cohen, P., & Spiegelman, B. M. (2011) · Nature reviews. Cancer · doi:10.1038/nrc3174 · Cited by 921
Curat, C. A., Wegner, V., Sengenès, C., Miranville, A., Tonus, C., Busse, R., & Bouloumié, A. (2006) · Diabetologia · doi:10.1007/s00125-006-0173-z · Cited by 693
Wu, H., Ghosh, S., Perrard, X. D., Feng, L., García, G., Perrard, J. L., Sweeney, J. F., Peterson, L. E., Chan, L., Smith, C. W., et al. (2007) · Circulation · doi:10.1161/circulationaha.106.638379 · Cited by 696
Henegar, C., Tordjman, J., Achard, V., Lacasa, D., Cremer, I., Guerre-Millo, M., Poitou, C., Basdevant, A., Stich, V., Viguerie, N., et al. (2008) · Genome Biology · doi:10.1186/gb-2008-9-1-r14 · Cited by 471
Shaul, M. E., Bennett, G., Strissel, K. J., Greenberg, A. S., & Obin, M. S. (2010) · Diabetes · doi:10.2337/db09-1402 · Cited by 383
Hui, X. H., Gu, P., Zhang, J., Nie, T., Pan, Y., Wu, D., Feng, T., Zhong, C., Wang, Y., Lam, K. S. L., et al. (2015) · Cell Metabolism · doi:10.1016/j.cmet.2015.06.004 · Cited by 355
Obesity and cancer: the role of adipose tissue and adipo-cytokines-induced chronic inflammation
Divella, R., Luca, R. D., Abbate, I., Naglieri, E., & Daniele, A. (2016) · Journal of Cancer · doi:10.7150/jca.16884 · Cited by 355
Adipose Tissue MicroRNAs as Regulators of CCL2 Production in Human Obesity
Arner, E., Mejhert, N., Kulyté, A., Balwierz, P. J., Pachkov, M. V., Cormont, M., Lorente‐Cebrián, S., Ehrlund, A., Laurencikiene, J., Hedén, P., et al. (2012) · Diabetes · doi:10.2337/db11-1508 · Cited by 337
Granneman, J. G., Li, P., Zhu, Z., & Lu, Y. (2005) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00009.2005 · Cited by 298
Awazawa, M., Ueki, K., Inabe, K., Yamauchi, T., Kubota, N., Kaneko, K., Kobayashi, M., Iwane, A., Sasako, T., Okazaki, Y., et al. (2011) · Cell Metabolism · doi:10.1016/j.cmet.2011.02.010 · Cited by 277
Cao, H., Polansky, M. M., & Anderson, R. A. (2007) · Archives of Biochemistry and Biophysics · doi:10.1016/j.abb.2006.12.034 · Cited by 281
Lee, Y. H., Nair, S., Rousseau, E., Allison, D. B., Page, G. P., Tataranni, P. A., Bogardus, C., & Permana, P. A. (2005) · Diabetologia · doi:10.1007/s00125-005-1867-3 · Cited by 265
Adipose cell size: importance in health and disease
Stenkula, K. G., & Erlanson‐Albertsson, C. (2018) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.00257.2017 · Cited by 266
Glucocorticoids and 11beta-Hydroxysteroid Dehydrogenase in Adipose Tissue
Seckl, J. R. (2004) · Recent Progress in Hormone Research · doi:10.1210/rp.59.1.359 · Cited by 239
“More plausibly, adipose tissue dissociation and culture lead to early induction of adipocyte cytokine synthesis and release (Ruan et al., 2003).”
Zhang, Y., Mei, H., Chang, X., Chen, F., Zhu, Y., & Han, X. (2016) · Journal of Molecular Cell Biology · doi:10.1093/jmcb/mjw040 · Cited by 222
Linscheid, P., Seboek, D., Zulewski, H., Keller, U. B., & Müller-Stich, B. P. (2005) · Endocrinology · doi:10.1210/en.2004-1424 · Cited by 197
Inhibition of p38MAPK Increases Adipogenesis From Embryonic to Adult Stages
Aouadi, M., Laurent, K., Prot, M., MARCHAND‐BRUSTEL, Y. L., Binétruy, B., & Bost, F. (2006) · Diabetes · doi:10.2337/diabetes.55.02.06.db05-0963 · Cited by 194
FAT CELLS: Afferent and Efferent Messages Define New Approaches to Treat Obesity
Lafontan, M. (2004) · The Annual Review of Pharmacology and Toxicology · doi:10.1146/annurev.pharmtox.45.120403.095843 · Cited by 191
Endocrine Protection of Ischemic Myocardium by FGF21 from the Liver and Adipose Tissue
Liu, S. Q., Roberts, D., Kharitonenkov, A., Zhang, B., Hanson, S., Li, Y. C., Zhang, L., & Wu, Y. (2013) · Scientific Reports · doi:10.1038/srep02767 · Cited by 179
The Human Visceral Fat Depot Has a Unique Inflammatory Profile
Alvehus, M., Burén, J., Sjöstróm, M., Goedecke, J. H., & Olsson, T. (2010) · Obesity · doi:10.1038/oby.2010.22 · Cited by 174
Roh, H. C., Tsai, L. T.-Y., Lyubetskaya, A., Tenen, D. E., Kumari, M., & Rosen, E. D. (2017) · Cell Reports · doi:10.1016/j.celrep.2016.12.087 · Cited by 150
Berry, D. C., Jacobs, H., Marwarha, G., Gely-Pernot, A., O'Byrne, S. M., DeSantis, D., Klopfenstein, M., Feret, B., Dennefeld, C., Blaner, W. S., et al. (2013) · Journal of Biological Chemistry · doi:10.1074/jbc.m113.484014 · Cited by 148
Effects of Different Hypocaloric Diets on Protein Secretion From Adipose Tissue of Obese Women
Nordström, E. A., Viguerie, N., Andersson, I., Verdich, C., Langin, D., & Arner, P. (2004) · Diabetes · doi:10.2337/diabetes.53.8.1966 · Cited by 139
Vasudevan, A. R., Wu, H., Xydakis, A. M., Jones, P. H., Smith, E. O., Sweeney, J. F., Corry, D. B., & Ballantyne, C. M. (2006) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2005-1280 · Cited by 132
Harms, M., Li, Q., Lee, S., Zhang, C., Kull, B., Hallén, S., Thorell, A., Alexandersson, I., Hagberg, C. E., Peng, X., et al. (2019) · Cell Reports · doi:10.1016/j.celrep.2019.03.026 · Cited by 137
Adipocytes: Impact on tumor growth and potential sites for therapeutic intervention
Hefetz-Sela, S., & Scherer, P. E. (2013) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2013.01.008 · Cited by 136
Deep Subcutaneous Adipose Tissue: A Distinct Abdominal Adipose Depot
Walker, G. E., Verti, B., Marzullo, P., Savia, G., Mencarelli, M., Zurleni, F., Liuzzi, A., & Blasio, A. M. D. (2007) · Obesity · doi:10.1038/oby.2007.231 · Cited by 129
Transcriptional regulation of the GLUT4 gene: from PPAR-γ and FOXO1 to FFA and inflammation
Armoni, M., Harel, C., & Karnieli, E. (2007) · Trends in Endocrinology and Metabolism · doi:10.1016/j.tem.2007.02.001 · Cited by 125
The NLRP3 Inflammasome as a Novel Player of the Intercellular Crosstalk in Metabolic Disorders
Benetti, E., Chiazza, F., Patel, N. S. A., & Collino, M. (2013) · Mediators of Inflammation · doi:10.1155/2013/678627 · Cited by 122
Brozinick, J. T., Hawkins, E. D., Strawbridge, A. B., & Elmendorf, J. S. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m402697200 · Cited by 120
Adipose tissue inflammation and cancer cachexia: Possible role of nuclear transcription factors
Júnior, M. L. B., Peres, S. B., McDonald, M. E., de Alcântara, P. S. M., Oliván, M., Otoch, J. P., Farmer, S. R., & Seelaender, M. C. L. (2011) · Cytokine · doi:10.1016/j.cyto.2011.10.008 · Cited by 113
Chatterjee, T. K., Aronow, B. J., Tong, W. S., Manka, D. R., Tang, Y., Bogdanov, V. Y., Unruh, D. J., Blomkalns, A. L., Piegore, M., Weintraub, D. S., et al. (2013) · Physiological Genomics · doi:10.1152/physiolgenomics.00042.2013 · Cited by 115
Metabolic Adaptations in the Absence of Perilipin
Saha, P., Kojima, H., Martı́nez-Botas, J., Sunehag, A. L., & Chan, L. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m405499200 · Cited by 105
Hajri, T., Tao, H., Wattacheril, J. J., Marks–Shulman, P. A., & Abumrad, N. N. (2010) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00307.2010 · Cited by 105
Adipocyte SIRT1 controls systemic insulin sensitivity by modulating macrophages in adipose tissue
Hui, X. H., Zhang, M., Gu, P., Li, K., Gao, Y. Z., Wu, D., Wang, Y., & Xu, A. (2017) · EMBO Reports · doi:10.15252/embr.201643184 · Cited by 99
Alterations of the classic pathway of complement in adipose tissue of obesity and insulin resistance
Zhang, J., Wright, W. S., Bernlohr, D., Cushman, S. W., & Chen, X. (2007) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00664.2006 · Cited by 96
Self-citationA Role for TRPV1 in Influencing the Onset of Cardiovascular Disease in Obesity
Marshall, N. J., Liang, L., Bodkin, J., Dessapt-Baradez, C., Nandi, M., Collot-Teixeira, S., Smillie, S.-J., Lalgi, K., Fernandes, E. S., Gnudi, L., et al. (2013) · Hypertension · doi:10.1161/hypertensionaha.112.201434 · Cited by 95
Starr, M. E., Saito, M., Evers, B. M., & Saitō, H. (2014) · The Journals of Gerontology Series A · doi:10.1093/gerona/glu197 · Cited by 90
The endocrine profile of subcutaneous and visceral adipose tissue of obese patients
Dolinkova, M., Dostálová, I., Lacinová, Z., Michalský, D., Haluzíková, D., Mráz, M., Kasalicky, M., & Haluzı́k, M. (2008) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2008.05.001 · Cited by 89
Danielsson, A., Öst, A., Lystedt, E., Kjølhede, P., Gustavsson, J., Nyström, F. H., & Strålfors, P. (2004) · FEBS Journal · doi:10.1111/j.1432-1033.2004.04396.x · Cited by 85
Adipocytes decrease Runx2 expression in osteoblastic cells: Roles of PPARγ and adiponectin
Liu, L., Shen, W., Zhang, Z. H., Wang, L., & Kraemer, F. B. (2010) · Journal of Cellular Physiology · doi:10.1002/jcp.22291 · Cited by 79
Tumor necrosis factor‐α and interleukin‐1β increases CTRP1 expression in adipose tissue
Kim, K.-Y., Kim, H. Y., Kim, J. H., Lee, C., Kim, D., Lee, Y. H., Han, S. H., Lim, J., Cho, D. H., Lee, M.-S., et al. (2006) · FEBS Letters · doi:10.1016/j.febslet.2006.06.034 · Cited by 80
Ding, Y., Wu, Y., Zeng, R., & Liao, K. (2012) · Acta Biochimica et Biophysica Sinica · doi:10.1093/abbs/gms008 · Cited by 74
SGK1 Kinase Upregulates GLUT1 Activity and Plasma Membrane Expression
Palmada, M., Boehmer, C., Akel, A., Rajamanickam, J., Jeyaraj, S., Keller, K., & Lang, F. (2006) · Diabetes · doi:10.2337/diabetes.55.02.06.db05-0720 · Cited by 71
Regulation of insulin sensitivity by adipose tissue-derived hormones and inflammatory cytokines
Ruan, H., & Lodish, H. F. (2004) · Current Opinion in Lipidology · doi:10.1097/00041433-200406000-00009 · Cited by 65
Self-citationTrujillo, M. E., Lee, M., Sullivan, S., Feng, J., Schneider, S. H., Greenberg, A. S., & Fried, S. K. (2006) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2005-1901 · Cited by 65
Multilayered omics reveal sex- and depot-dependent adipose progenitor cell heterogeneity
Shan, B., Barker, C. S., Shao, M., Zhang, Q., Gupta, R. K., & Wu, Y. (2022) · Cell Metabolism · doi:10.1016/j.cmet.2022.03.012 · Cited by 67
Roh, H. C., Kumari, M., Taleb, S., Tenen, D., Jacobs, C., Lyubetskaya, A., Tsai, L. T.-Y., & Rosen, E. D. (2020) · Molecular Metabolism · doi:10.1016/j.molmet.2020.101086 · Cited by 62
Estrogen Sulfotransferase Inhibits Adipocyte Differentiation
Wada, T., Ihunnah, C. A., Gao, J., Chai, X., Zeng, S., Philips, B. J., Rubin, J. P., Marra, K. G., & Xie, W. (2011) · Molecular Endocrinology · doi:10.1210/me.2011-1089 · Cited by 54
Esteves, J. V. D. C., Yonamine, C. Y., Júnior, D. A. C. P., Gerlinger‐Romero, F., Enguita, F. J., & Machado, U. F. (2018) · Frontiers in Endocrinology · doi:10.3389/fendo.2018.00536 · Cited by 59
Programming of mouse obesity by maternal exposure to concentrated ambient fine particles
Chen, M., Wang, X., Hu, Z., Zhou, H., Xu, Y., Qiu, L., Qin, X., Zhang, Y., & Ying, Z. (2017) · Particle and Fibre Toxicology · doi:10.1186/s12989-017-0201-9 · Cited by 59
Shao, L., Zhou, H. J., Zhang, H., Qin, L., Hwa, J., Yun, Z., Ji, W., & Wang, M. (2015) · Nature Communications · doi:10.1038/ncomms9917 · Cited by 61
Beard, K. M., Lu, H., Ho, K., & Fantus, I. G. (2006) · Diabetes · doi:10.2337/db05-1538 · Cited by 57
Obesity as an important risk factor for certain types of cancer.
Davoodi, S. H., Malekshahabi, T., Moghadam, A. M., Shahbazi, R., & Esmaeili, S. (2013) · Europe PMC (PubMed Central) · Cited by 54
Nie, Y., Ma, R. C., Chan, J. C. N., Xu, H., & Xu, G. (2012) · The FASEB Journal · doi:10.1096/fj.11-196782 · Cited by 53
Dreja, T., Jovanovic, Z., Rasche, A., Kluge, R., Herwig, R., Tung, Y. C. L., Joost, H., Yeo, G. S. H., & Al‐Hasani, H. (2009) · Diabetologia · doi:10.1007/s00125-009-1576-4 · Cited by 53
Insulin Increases Tristetraprolin and Decreases VEGF Gene Expression in Mouse 3T3–L1 Adipocytes
Cao, H., Urban, J. F., & Anderson, R. A. (2008) · Obesity · doi:10.1038/oby.2008.65 · Cited by 52
Lindholm, A., Blomquist, C. H., Bixo, M., Dahlbom, I., Hansson, T., Poromaa, I. S., & Burén, J. (2011) · Human Reproduction · doi:10.1093/humrep/der096 · Cited by 51
The anticancer and antiobesity effects of Mediterranean diet
Kwan, H. Y., Chao, X., Su, T., Fu, X., Tse, A. K., Fong, W. F., & Yu, Z. (2015) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2013.852510 · Cited by 51
Kishore, P., Li, W., Tonelli, J., Lee, D.-E., Koppaka, S., Zhang, K., Lin, Y., Kehlenbrink, S., Scherer, P. E., & Hawkins, M. A. (2010) · Science Translational Medicine · doi:10.1126/scitranslmed.3000292 · Cited by 48
LBP-4a improves insulin resistance via translocation and activation of GLUT4 in OLETF rats
Zhao, R., Qiu, B., Li, Q., Zhang, T., Zhao, H., Chen, Z., Cai, Y., Ruan, H., Ge, W.-Z., & Zheng, X. (2014) · Food & Function · doi:10.1039/c3fo60602c · Cited by 47
Self-citationHo, J., Choi, J., Lim, W., Kim, M., Lee, I., & Cho, H. (2012) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.5792 · Cited by 46
Wagner, G., Lindroos‐Christensen, J., Einwallner, E., Husa, J., Zapf, T.-C., Lipp, K., Rauscher, S., Gröger, M., Spittler, A., Loewe, R. P., et al. (2017) · Scientific Reports · doi:10.1038/srep40881 · Cited by 46
Renström, F., Burén, J., Svensson, M. K. E., & Eriksson, J. W. (2007) · Metabolism · doi:10.1016/j.metabol.2006.09.012 · Cited by 46
Multi-tissue gene-expression analysis in a mouse model of thyroid hormone resistance
Miller, L. D., McPhie, P., Suzuki, H., Kato, Y., Liu, E. T., & Cheng, S.-Y. (2004) · Genome biology · doi:10.1186/gb-2004-5-5-r31 · Cited by 46
The Multi-Level Action of Fatty Acids on Adiponectin Production by Fat Cells
Karki, S., Chakrabarti, P., Huang, G., Wang, H., Farmer, S. R., & Kandror, K. V. (2011) · PLoS ONE · doi:10.1371/journal.pone.0028146 · Cited by 45
Virtue, S., Masoodi, M., de Weijer, B. A., van Eijk, M., Mok, C. Y. L., Eiden, M. J., Dale, M. M., Pirraco, A., Serlie, M. J. M., Griffin, J. L., et al. (2015) · International Journal of Obesity · doi:10.1038/ijo.2015.34 · Cited by 44
Balasubramanian, R., Robaye, B., Boeynaems, J., & Jacobson, K. A. (2014) · PLoS ONE · doi:10.1371/journal.pone.0116203 · Cited by 42
Fain, J. N., Bahouth, S. W., & Madan, A. K. (2005) · Biochemical Pharmacology · doi:10.1016/j.bcp.2005.02.009 · Cited by 41
Park, J., Oh, S., & Cha, Y. (2014) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.6588 · Cited by 40
Kovsan, J., Osnis, A., Maissel, A., Mazor, L., Tarnovscki, T., Hollander, L., Ovadia, S., Meier, B., Klein, J. C., Bashan, N., et al. (2008) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.90486.2008 · Cited by 40
Schook, M. W., Wildt, D. E., Raghanti, M. A., Wolfe, B. A., & Dennis, P. M. (2015) · General and Comparative Endocrinology · doi:10.1016/j.ygcen.2015.05.003 · Cited by 39
Koška, J., Stefan, N., Dubois, S. G., Trinidad, C., Considine, R. V., Funahashi, T., Bunt, J. C., Ravussin, É., & Permana, P. A. (2009) · International Journal of Obesity · doi:10.1038/ijo.2009.106 · Cited by 40
Nitta, C. F., & Orlando, R. A. (2013) · PLoS ONE · doi:10.1371/journal.pone.0077306 · Cited by 42
Ribé, D., Yang, J., Patel, S. V., Koumanov, F., Cushman, S. W., & Holman, G. D. (2005) · Endocrinology · doi:10.1210/en.2004-1294 · Cited by 38
Self-citationAssociations Between Obesity and Cancer: The Role of Fatty Acid Synthase
Wang, D., & DuBois, R. N. (2012) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/djs010 · Cited by 36
MAPKs/AP-1, not NF-κB, is responsible for MCP-1 production in TNF-α-activated adipocytes
Zhang, X., Liu, Z., Li, W., Kang, Y., Xu, Z., Li, X., Gao, Y., & Qi, Y. (2022) · Adipocyte · doi:10.1080/21623945.2022.2107786 · Cited by 32
Obesity and cholangiocarcinoma
Parsi, M. A. (2013) · World Journal of Gastroenterology · doi:10.3748/wjg.v19.i4.457 · Cited by 32
Esteves, C. L., Kelly, V., Breton, A., Taylor, A. I., West, C. C., Donadeu, F. X., Péault, B., Seckl, J. R., & Chapman, K. E. (2013) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2013-1708 · Cited by 31
Early breast cancer: why does obesity affect prognosis?
Heetun, A., Cutress, R., & Copson, E. (2018) · Proceedings of The Nutrition Society · doi:10.1017/s0029665118000447 · Cited by 28
RNAi-based gene silencing in primary mouse and human adipose tissues
Puri, V., Chakladar, A., Virbasius, J. V., Konda, S., Powelka, A. M., Chouinard, M., Hagan, G. N., Perugini, R. A., & Czech, M. (2006) · Journal of Lipid Research · doi:10.1194/jlr.d600033-jlr200 · Cited by 28
Evaluation of macrophage plasticity in brown and white adipose tissue
Ortega, M. T., Xie, L., Mora, S., & Chapes, S. K. (2011) · Cellular Immunology · doi:10.1016/j.cellimm.2011.06.012 · Cited by 27
Yang, F., Duan, M., Zheng, F., Yu, L., Wang, Y., Wang, G., Lin, J., Han, S., Gan, D., Meng, Z., et al. (2021) · Cancer Letters · doi:10.1016/j.canlet.2021.09.024 · Cited by 27
Ex vivo analysis of renal proximal tubular cells
Legouis, D., Bataille, A., Hertig, A., Vandermeersch, S., Simon‐Tillaux, N., Rondeau, É., & Galichon, P. (2015) · BMC Cell Biology · doi:10.1186/s12860-015-0058-4 · Cited by 27
Wu, Y., Kim, J. Y., Zhou, S., & Smas, C. M. (2008) · BMC Genomics · doi:10.1186/1471-2164-9-397 · Cited by 27
Therapeutic Doses of Multipotent Stromal Cells from Minimal Adipose Tissue
Zhang, N., Dietrich, M. A., & Lopez, M. J. (2014) · Stem Cell Reviews and Reports · doi:10.1007/s12015-014-9508-1 · Cited by 25
Oxidation of high doses of serotonin favors lipid accumulation in mouse and human fat cells
Grès, S., Canteiro, S., Mercader, J., & Carpéné, C. (2013) · Molecular Nutrition & Food Research · doi:10.1002/mnfr.201200681 · Cited by 23
Minard, A. Y., Wong, M. K. L., Chaudhuri, R., Tan, S., Humphrey, S. J., Parker, B. L., Yang, J. Y. H., Laybutt, D. R., Cooney, G. J., Coster, A. C. F., et al. (2016) · Journal of Biological Chemistry · doi:10.1074/jbc.m116.741140 · Cited by 23
- Invited Review - Physiological Roles of Adipokines, Hepatokines, and Myokines in Ruminants
Roh, S., Suzuki, Y., Gotoh, T., Tatsumi, R., & Katoh, K. (2015) · Asian-Australasian Journal of Animal Sciences · doi:10.5713/ajas.16.0001r · Cited by 24
Albumin inhibits adipogenesis and stimulates cytokine release from human adipocytes
Schlesinger, J. B., van Harmelen, V., Alberti-Huber, C., & Hauner, H. (2006) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00172.2005 · Cited by 23
Koška, J., de Courten, B. V., Wake, D. J., Nair, S., Walker, B. R., Bunt, J. C., Permana, P. A., Lindsay, R. S., & Tataranni, P. A. (2006) · Obesity · doi:10.1038/oby.2006.175 · Cited by 23
Rheumatoid Arthritis Treatment Options and Type 2 Diabetes: Unravelling the Association
Muzio, C. D., Cipriani, P., & Ruscitti, P. (2022) · BioDrugs · doi:10.1007/s40259-022-00561-7 · Cited by 26
A chromatin immunoprecipitation (ChIP) protocol for use in whole human adipose tissue
Haim, Y., Tarnovscki, T., Bashari, D., & Rudich, A. (2013) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00598.2012 · Cited by 21
Elenkova, M., Tipton, D. A., Karydis, A., & Stein, S. H. (2018) · Journal of Periodontal Research · doi:10.1111/jre.12613 · Cited by 21
Metabolic effects of leptin receptor knockdown or reconstitution in adipose tissues
Pereira, S., O’Dwyer, S. M., Webber, T. D., Baker, R. K., So, V., Ellis, C. E., Yoon, J. S., Mojibian, M., Glavas, M. M., Karunakaran, S., et al. (2019) · Scientific Reports · doi:10.1038/s41598-019-39498-3 · Cited by 24
Kim, J. Y., Wu, Y., & Smas, C. M. (2007) · Physiological Genomics · doi:10.1152/physiolgenomics.00206.2006 · Cited by 19
Metabolic disorders and the risk of cholangiocarcinoma
Ghidini, M., Ramai, D., Facciorusso, A., Singh, J. K., Tai, W., Rijavec, E., Galassi, B., Grossi, F., & Indini, A. (2021) · Expert Review of Gastroenterology & Hepatology · doi:10.1080/17474124.2021.1946393 · Cited by 19
Westergren, H., Danielsson, A., Nyström, F. H., & Strålfors, P. (2005) · Metabolism · doi:10.1016/j.metabol.2005.01.021 · Cited by 18
Metadata
- Authors
- Ruan, H., Zarnowski, M. J., Cushman, S. W., & Lodish, H. F.
- Affiliations
- Whitehead Institute for Biomedical Research (US)
- National Institutes of Health (US)
- Corresponding author
- Lodish, Harvey F.
- ORCID
- Lodish, Harvey F.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2003-11
- Volume
- 278
- Issue
- 48
- Pages
- 47585-47593
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2016692980
- Cited by
- 168
- References
- 31
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 3 · 2023: 5 · 2022: 6 · 2021: 2 · 2020: 2 · 2019: 3 · 2018: 12 · 2017: 8 · 2016: 4 · 2015: 12 · 2014: 5 · 2013: 17 · 2012: 9
- FWCI
- 7.48
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Howard Hughes Medical Institute
- American Diabetes Association
- National Institutes of Health
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adipokines, Inflammation, and Metabolic Diseases
- Health Sciences › Medicine › Physiology (medical) › Adipose Tissue and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
- Keywords
- epididymal adipose tissue, inflammatory mediators, TNF-α, insulin resistance, GLUT1, adipocyte gene expression, NF-κB, collagenase digestion, TNF-alpha knockout mice, inflammatory cytokines
- MeSH terms
- Epidermal Cells, Adipose Tissue, Animals, Cell Adhesion, Cells, Cultured, Dose-Response Relationship, Drug, Enzyme-Linked Immunosorbent Assay, Glucose, Inflammation, Insulin, Interleukin-1, Mice, Inbred C57BL, Monosaccharide Transport Proteins, Muscle Proteins, Oligonucleotides, RNA, Messenger, Time Factors, Tumor Necrosis Factor-alpha, Signal Transduction, Down-Regulation, Interleukin-6, NF-kappa B, Collagenases, Adipocytes, RNA, Complementary, Chemokines, Reverse Transcriptase Polymerase Chain Reaction, Oligonucleotide Array Sequence Analysis, 3T3-L1 Cells, Glucose Transporter Type 1, Glucose Transporter Type 4, Mice, In Vitro Techniques
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
