The DOI matches its Crossref record; 53 of the 53 references it cites were found in a registry or an index.
Calpain I Induces Cleavage and Release of Apoptosis-inducing Factor from Isolated Mitochondria
Open accessAbstract
The translocation of apoptosis-inducing factor (AIF) from mitochondria to the nucleus has been implicated in the mechanism of glutamate excitotoxicity in cortical neurons and has been observed in vivo following acute rodent brain injuries. However, the mechanism and time course of AIF redistribution to the nucleus is highly controversial. Because elevated intracellular calcium is one of the most ubiquitous features of neuronal cell death, this study tested the hypothesis that cleavage of AIF by the calcium-activated protease calpain mediates its release from mitochondria. Both precursor and mature forms of recombinant AIF were cleaved near the amino terminus by calpain I in vitro. Mitochondrial outer membrane permeabilization by truncated Bid induced cytochrome c release from isolated liver or brain mitochondria but only induced AIF release in the presence of active calpain. Enzymatic inhibition of calpain by calpeptin precluded AIF release, demonstrating that proteolytic activity was required for release. Calpeptin and the mitochondrial permeability transition pore antagonist cyclosporin A also inhibited calcium-induced AIF release from mouse liver mitochondria, implicating the involvement of an endogenous mitochondrial calpain in release of AIF during permeability transition. Cleavage of AIF directly decreased its association with pure lipid vesicles of mitochondrial inner membrane composition. Taken together, these results define a novel mechanism of AIF release involving calpain processing and identify a potential molecular checkpoint for cytoprotective interventions.
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Cites
Phosphorus Assay in Column Chromatography
Bartlett, G. R. (1959) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)70226-3 · Cited by 12,801
Molecular characterization of mitochondrial apoptosis-inducing factor
Susin, S. A., Lorenzo, H. K., Zamzami, N., Marzo, I., Snow, B. E., Brothers, G. M., Mangion, J., Jacotot, E., Costantini, P., Loeffler, M., et al. (1999) · Nature · doi:10.1038/17135 · Cited by 4,060
GOLL, D. E., THOMPSON, V. F., LI, H., WEI, W., & CONG, J. (2003) · Physiological Reviews · doi:10.1152/physrev.00029.2002 · Cited by 2,829
Mediation of Poly(ADP-Ribose) Polymerase-1-Dependent Cell Death by Apoptosis-Inducing Factor
Yu, S.-W., Wang, H., Poitras, M. F., Coombs, C., Bowers, W. J., Federoff, H. J., Poirier, G. G., Dawson, T. M., & Dawson, V. L. (2002) · Science · doi:10.1126/science.1072221 · Cited by 1,826
PEST sequences and regulation by proteolysis
Rechsteiner, M., & Rogers, S. W. (1996) · Trends in Biochemical Sciences · doi:10.1016/s0968-0004(96)10031-1 · Cited by 1,640
Bid, Bax, and Lipids Cooperate to Form Supramolecular Openings in the Outer Mitochondrial Membrane
Kuwana, T., Mackey, M. R., Perkins, G., Ellisman, M. H., Latterich, M., Schneiter, R., Green, D. R., & Newmeyer, D. D. (2002) · Cell · doi:10.1016/s0092-8674(02)01036-x · Cited by 1,478
tBID, a membrane-targeted death ligand, oligomerizes BAK to release cytochrome c
Wei, M. C., Lindsten, T., Mootha, V. K., Weiler, S., Gross, A., Ashiya, M., Thompson, C. B., & Korsmeyer, S. J. (2000) · Genes & Development · doi:10.1101/gad.14.16.2060 · Cited by 1,268
Neurotoxicity induces cleavage of p35 to p25 by calpain
Lee, M.-S., Kwon, Y. T., Li, M., Peng, J., Friedlander, R. M., & Tsai, L.-H. (2000) · Nature · doi:10.1038/35012636 · Cited by 1,063
Arnoult, D. (2003) · The EMBO Journal · doi:10.1093/emboj/cdg423 · Cited by 451
Mitochondrial contact sites. Lipid composition and dynamics.
Ardail, D., Privat, J. P., Egret-Charlier, M., Levrat, C., Lerme, F., & Louisot, P. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)30583-5 · Cited by 445
Cited by
Mitochondrial membrane permeabilization in cell death
Kroemer, G., Galluzzi, L., & Brenner, C. (2007) · Physiological Reviews · doi:10.1152/physrev.00013.2006 · Cited by 3,598
Mitochondria and cell death: outer membrane permeabilization and beyond
Tait, S. W. G., & Green, D. R. (2010) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2952 · Cited by 2,593
Cell death by necrosis: towards a molecular definition
Golstein, P., & Kroemer, G. (2007) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2006.11.001 · Cited by 1,044
Cell death modalities: classification and pathophysiological implications
Galluzzi, L., Maiuri, M. C., Vitale, I., Zischka, H., Castedo, M., Zitvogel, L., & Kroemer, G. (2007) · Cell Death & Differentiation · doi:10.1038/sj.cdd.4402148 · Cited by 832
Mitochondrial Regulation of Cell Death
Tait, S. W. G., & Green, D. R. (2013) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a008706 · Cited by 530
Metadata
- Authors
- Polster, B. M., Basañez, G., Etxebarria, A., Hardwick, J. M., & Nicholls, D. G.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2005-02
- Volume
- 280
- Issue
- 8
- Pages
- 6447-6454
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2032444047
- Cited by
- 437
- References
- 53
- FWCI
- 9.97
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Polster, B. M., Basañez, G., Etxebarria, A., Hardwick, J. M., & Nicholls, D. G. (2005). Calpain I Induces Cleavage and Release of Apoptosis-inducing Factor from Isolated Mitochondria. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.m413269200
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