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Proteomics Characterization of Mouse Kidney Peroxisomes by Tandem Mass Spectrometry and Protein Correlation Profiling
Open accessAbstract
The peroxisome represents a ubiquitous single membrane-bound key organelle that executes various metabolic pathways such as fatty acid degradation by alpha- and beta-oxidation, ether-phospholipid biosynthesis, metabolism of reactive oxygen species, and detoxification of glyoxylate in mammals. To fulfil this vast array of metabolic functions, peroxisomes accommodate approximately 50 different enzymes at least as identified until now. Interest in peroxisomes has been fueled by the discovery of a group of genetic diseases in humans, which are caused by either a defect in peroxisome biogenesis or the deficient activity of a distinct peroxisomal enzyme or transporter. Although this research has greatly improved our understanding of peroxisomes and their role in mammalian metabolism, deeper insight into biochemistry and functions of peroxisomes is required to expand our knowledge of this low abundance but vital organelle. In this work, we used classical subcellular fractionation in combination with MS-based proteomics methodologies to characterize the proteome of mouse kidney peroxisomes. We could identify virtually all known components involved in peroxisomal metabolism and biogenesis. Moreover through protein localization studies by using a quantitative MS screen combined with statistical analyses, we identified 15 new peroxisomal candidates. Of these, we further investigated five candidates by immunocytochemistry, which confirmed their localization in peroxisomes. As a result of this joint effort, we believe to have compiled the so far most comprehensive protein catalogue of mammalian peroxisomes.
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“Previous proteomic studies using isolated mammalian peroxisomes have identified ACBD5 as a peroxisomal protein (16,17).”
From Deficiency of a Retinal Dystrophy Protein, Acyl-CoA Binding Domain-containing 5 (ACBD5), Impairs Peroxisomal β-Oxidation of Very-long-chain Fatty Acids (2017) · cites this paper as [17]
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“Only four candidates (ZADH2, ACAD11, ABHD14B, and a protein similar to BACAT) contain a peroxisomal targeting signal type 1 (PTS1) as predicted by using PSORT (58).”
MethodProteomic characterization of the human centrosome by protein correlation profiling
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Self-citationQuantitation in Mass-Spectrometry-Based Proteomics
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Reumann, S., Quan, S., Aung, K., Yang, P., Manandhar-Shrestha, K., Holbrook, D., Linka, N., Switzenberg, R., Wilkerson, C. G., Weber, A. P. M., et al. (2009) · PLANT PHYSIOLOGY · doi:10.1104/pp.109.137703 · Cited by 262
ACBD5 and VAPB mediate membrane associations between peroxisomes and the ER
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Metadata
- Authors
- Wiese, S., Gronemeyer, T., Ofman, R., Kunze, M., Grou, C. P., Almeida, J. A., Eisenacher, M., Stephan, C., Hayen, H., Schollenberger, L., Korosec, T., Waterham, H. R., Schliebs, W., Erdmann, R., Berger, J., Meyer, H. E., Just, W., Azevedo, J. E., Wanders, R. J. A., Warscheid, B., & Grou, C. P.
- Affiliations
- Ruhr University Bochum (DE)
- Universidade do Porto (PT)
- Amsterdam UMC Location University of Amsterdam (NL)
- Medical University of Vienna (AT)
- Instituto de Biologia Molecular e Celular (PT)
- Heidelberg University (DE)
- Corresponding author
- Warscheid, Bettina
- ORCID
- Venue
- Molecular & Cellular Proteomics
- Publisher
- Elsevier BV
- Published
- 2007-12
- Volume
- 6
- Issue
- 12
- Pages
- 2045-2057
- ISSN
- 1535-9476 (ISSN-L)
- OpenAlex
- W2121085194
- Cited by
- 231
- References
- 70
- Citations by year
- 2026: 3 · 2025: 5 · 2024: 10 · 2023: 17 · 2022: 11 · 2021: 5 · 2020: 6 · 2019: 5 · 2018: 16 · 2017: 11 · 2016: 16 · 2015: 16 · 2014: 14 · 2013: 18 · 2012: 14
- FWCI
- 7.84
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Molecular & Cellular Proteomics (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Pure Amsterdam UMC (submittedVersion)
- Funders
- European Commission (LSHG-CT-2004-512018)
- Deutsche Forschungsgemeinschaft
- Austrian Science Fund
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Metabolism and Genetic Disorders
- Keywords
- peroxisomes, mouse kidney, peroxisome biogenesis, peroxisomal enzymes, peroxisomal proteins, protein localization, peroxisomal disorders, fatty acid oxidation, reactive oxygen species metabolism
- MeSH terms
- Animals, Chromatography, High Pressure Liquid, Kidney, Peroxisomes, Spectrometry, Mass, Electrospray Ionization, Proteomics, Mice, Tandem Mass Spectrometry
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