Cited by 143Advanced Proteomics Techniques and ApplicationsBioinformatics and Genomic NetworksBiotin and Related Studies

Identification of Protein Interactions Involved in Cellular Signaling

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Westermarck, Jukka, Ivaska, Johanna, Corthals, Garry L.·2013·Molecular & Cellular Proteomics

Abstract

Protein-protein interactions drive biological processes. They are critical for all intra- and extracellular functions, and the technologies to analyze them are widely applied throughout the various fields of biological sciences. This study takes an in-depth view of some common principles of cellular regulation and provides a detailed account of approaches required to comprehensively map signaling protein-protein interactions in any particular cellular system or condition. We provide a critical review of the benefits and disadvantages of the yeast two-hybrid method and affinity purification coupled with mass spectrometric procedures for identification of signaling protein-protein interactions. In particular, we emphasize the quantitative and qualitative differences between tandem affinity and one-step purification (such as FLAG and Strep tag) methods. Although applicable to all types of interaction studies, a special section is devoted in this review to aspects that should be considered when attempting to identify signaling protein interactions that often are transient and weak by nature. Finally, we discuss shotgun and quantitative information that can be gleaned by MS-coupled methods for analysis of multiprotein complexes.

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Crossref · 10.1074/mcp.r113.027771

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  • CNP and ATP5PO may be functionally related or operate together to mediate phenotypic features associated with UDN-P9's disease and hypomyelinating leukodystropy-20.

    “suggesting that they may be functionally related69–71 or operate together72,73 to mediate phenotypic features associated with UDN-P9’s disease and hypomyelinating leukodystropy-20”

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Authors
Westermarck, J., Ivaska, J., & Corthals, G. L.
Affiliations
Åbo Akademi University (FI)
Corresponding author
Corthals, Garry L.
Venue
Molecular & Cellular Proteomics
Publisher
Elsevier BV
Published
2013-07
Volume
12
Issue
7
Pages
1752-1763
ISSN
1535-9476 (ISSN-L)
OpenAlex
W2147130215
Cited by
143
References
101
Citations by year
2026: 21 · 2025: 19 · 2024: 15 · 2023: 8 · 2022: 13 · 2021: 12 · 2020: 8 · 2019: 12 · 2018: 7 · 2017: 5 · 2016: 7 · 2015: 4 · 2014: 9 · 2013: 1 · 2012: 1
FWCI
0.66
Type
journal article
Language
en
Open access
Locations
  • Molecular & Cellular Proteomics (publishedVersion, cc-by)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bioinformatics and Genomic Networks
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Biotin and Related Studies
Keywords
protein-protein interaction, signal transduction, yeast two-hybrid, affinity purification, tandem affinity purification, FLAG tag, Strep-tag, protein complexes, protein interaction mapping
MeSH terms
Humans, Mass Spectrometry, Signal Transduction, Two-Hybrid System Techniques, Protein Interaction Mapping

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