Cell-free extracts of Lactobacillus acidophilus and Lactobacillus delbrueckii display antiproliferative and antioxidant activities against HT-29 cell line
Abstract
Many beneficial effects of probiotic Lactobacilli on cancer prevention and therapy were previously presented. So finding probiotics with proapoptotic activities is a promising approach for cancer drug discovery. Here, the antiproliferative and antioxidant activities of cell-free extracts of Lactobacillus acidophilus and Lactobacillus delbrueckii on HT-29 cell line were evaluated employing MTT and DPPH assays. The induction of apoptosis was assessed by Hoechst staining and flow cytometry analysis which was further confirmed by expression analysis of BCL-2, BAX, caspase-3, caspase-8, and caspase-9 genes using real-time quantitative PCR. Caspase-3 activity was also analyzed. Results showed that cell viability was significantly reduced to 42.2 ± 0.01% and 19.40 ± 0.01% by 5 and 8 mg ml−1 of L. acidophilus and L. delbrueckii extracts, respectively. Apoptosis induction was shown with both bacterial extracts. Caspase-9 and caspase-3 overexpression as well as Bax/Bcl-2 ratio increase revealed the ability of both probiotics to induce intrinsic pathway-dependent apoptosis. The extrinsic pathway was also activated by L. acidophilus. At the concentration of 198 µg ml−1, L. acidophilus and L. delbrueckii had a DPPH scavenging activity of 59.37 ± 3.97% and 71.19 ± 3.64%, respectively. Taken together, these findings provide evidence for antiproliferative, proapoptotic, and antioxidant effects driven by these probiotic lactic acid bacteria (LAB) strains.
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The enhanced effect of S. salivarius CP163 and S. salivarius CP208 included the induction of colon cancer cells as partially mediated by activation of caspase-2.
“The stimulation of the death of colon cancer cells via intrinsic caspase-3 was observed after inducing a response from probiotic strains of L. delbrueckii and L. acidophilus [56,57], indicating that the enhanced effect of probiotic strains of S. salivarius CP163 and S. salivarius CP208 included the induction of colon cancer cells as partially mediated by activation of caspase-2.”
From Bioprophylactic potential of novel human colostrum probiotics via apoptotic induction of colon cancer cells and cell immune activation (2022) · cites this paper as [57]
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- Authors
- Baghbani-Arani, F., Asgary, V., & Hashemi, A.
- Affiliations
- Department of Genetics and Biotechnology, School of Biological Science, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran;
- Research and Development Laboratory, Javid Biotechnology Institute, Tehran, Iran;
- Shahid Beheshti University (IR)
- Corresponding author
- Hashemi, Atieh
- Venue
- Nutrition and Cancer
- Publisher
- Informa UK Limited
- Published
- 2019-11-11
- Volume
- 72
- Issue
- 8
- Pages
- 1390-1399
- ISSN
- 0163-5581 (ISSN-L)
- 1532-7914
- OpenAlex
- W2989471528
- Cited by
- 22
- References
- 30
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 6 · 2023: 4 · 2022: 4 · 2021: 3 · 2020: 2
- FWCI
- 1.74
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Nutrition and Cancer (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Cancer Research and Treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
- Keywords
- Lactobacillus acidophilus, Lactobacillus delbrueckii, cell-free extracts, probiotics, HT-29 cells, colorectal cancer, apoptosis, antiproliferative activity, antioxidant activity, caspase-3, caspase-9, Bax/Bcl-2 ratio, mitochondrial apoptosis, extrinsic apoptotic pathway, lactic acid bacteria
- MeSH terms
- Antineoplastic Agents, Antioxidants, Colonic Neoplasms, Humans, Lactobacillus acidophilus, Apoptosis, HT29 Cells, Probiotics, Lactobacillus delbrueckii, Cell Proliferation
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