Solid Phase Radioimmunoassay for Direct Measurement of Human Plasma Oxytocin
Abstract
Synthetic oxytocin (OT) conjugated to bovine thyroglobulin by the carbodiimide reaction was injected into rabbits to raise a high titre, specific OT antiserum which was then coupled to microcrystalline cellulose activated by cyanogen bromide. High affinity of the coupled antiserum was defined by Scatchard analysis, Keq = 7.1 X 10(11)1/mol. Cross-reactivity studies revealed little binding of antiserum to analogues of OT. Iodination was performed by the Chloramine T method, giving specific activity of 125I-OT, range 1.1 - 1.7 X 10(3) Ci/mmol. After incubation for 40 hours under disequilibrium conditions, specific and non-specific bindings were 10.6 +/- 2.7% and 0.2 +/- 0.1% (n=15), respectively. Displacement of 50% 125I-OT occurred with 2.9 pg OT/tube. Coefficients of variation of standard OT concentrations (0.03 - 16 pg/tube) were less than 5%. Limit of detection was 2 pg OT/ml plasma. Recovery of synthetic OT added to non-pregnant plasma was 81.8% (n=34) at 20 pg/ml and 97.4% (n=32) at 100 pg/ml. Two patients, 17 and 18 weeks post-partum, had increases in plasma OT from less than 2 pg/ml to 18.3 and 16.0 pg/ml after 6 and 4 minutes breast feeding infants, respectively. We conclude that this solid phase OT radioimmunoassay is quick, relatively sensitive and reliable, and does not require prior extraction of plasma samples.
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By the 1980s, many laboratories had generated radioimmunoassays for oxytocin using independently produced antibodies, and most but not all required extraction.
“By the 1980s, many laboratories had generated radioimmunoassays for oxytocin, using independently produced antibodies 58, 72, 73, 74, 94, 95, 96, 97, 98] and most, but not all 99, 100, required extraction.”
From Measuring Oxytocin and Vasopressin: Bioassays, Immunoassays and Random Numbers (2016) · cites this paper as [99]
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- Authors
- Burd, J. M., Weightman, D. R., & Baylis, P. H.
- Affiliations
- Royal Victoria Infirmary (GB)
- Venue
- Journal of Immunoassay
- Publisher
- Informa UK Limited
- Published
- 1985-09
- Volume
- 6
- Issue
- 3
- Pages
- 227-243
- ISSN
- 0197-1522 (ISSN-L)
- OpenAlex
- W1999981074
- Cited by
- 13
- References
- 16
- Citations by year
- 2025: 1 · 2016: 1 · 2013: 1
- FWCI
- 1.92
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Immunoassay (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced Biosensing Techniques and Applications
- Keywords
- oxytocin, solid-phase radioimmunoassay, bovine thyroglobulin, iodination, displacement assay, breastfeeding
- MeSH terms
- Antibody Specificity, Cross Reactions, Dose-Response Relationship, Immunologic, Female, Humans, Immunosorbent Techniques, Lactation, Oxytocin, Pregnancy, Radioimmunoassay
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