THE DEVELOPMENT OF A RADIOIMMUNOASSAY FOR OXYTOCIN: RADIO-IODINATION, ANTIBODY PRODUCTION AND SEPARATION TECHNIQUES
Abstract
SUMMARY: A simple and rapid method is described for labelling oxytocin with 131I at a high specific activity. This method is compared with those of previous workers. A satisfactory antiserum has been raised by direct intra-lymph node injection of oxytocin adsorbed to carbon microparticles. A number of methods for separating antibody-bound from free oxytocin are described, and reasons given for preferring a procedure using ammonium sulphate precipitation. These data form the basis for developing a radioimmunoassay intended for the determination of oxytocin in human plasma.
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The human posterior pituitary contains approximately 21 μg of oxytocin, whereas that of the rat contains 0.5-1 μg.
“the human posterior pituitary contains approximately 21 μg of oxytocin 72, 73, 74, whereas that of the rat contains 0.5–1 μg.”
From Measuring Oxytocin and Vasopressin: Bioassays, Immunoassays and Random Numbers (2016) · cites this paper as [74]
By the 1980s, many laboratories had generated radioimmunoassays for oxytocin using independently produced antibodies, and most but not all required extraction.
“By the 1980s, many laboratories had generated radioimmunoassays for oxytocin, using independently produced antibodies 58, 72, 73, 74, 94, 95, 96, 97, 98] and most, but not all 99, 100, required extraction.”
From Measuring Oxytocin and Vasopressin: Bioassays, Immunoassays and Random Numbers (2016) · cites this paper as [74]
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Vandesande, F., & Dierickx, K. (1975) · Cell and Tissue Research · doi:10.1007/bf00218970 · Cited by 496 · see citing works
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Vasicka, A., Kumaresan, P., Han, G. S., & Kumaresan, M. (1978) · American Journal of Obstetrics and Gynecology · doi:10.1016/0002-9378(78)90493-3 · Cited by 74 · see citing works
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Seppälä, M. T., Aho, I., Tissari, A. H., & Ruoslahti, E. I. (1972) · American Journal of Obstetrics and Gynecology · doi:10.1016/0002-9378(72)90902-7 · Cited by 34 · see citing works
Grzegorzewski, W. J., Skipor, J., Wasowska, B. A., & Krzymowski, T. (1995) · Biology of Reproduction · doi:10.1095/biolreprod52.1.139 · Cited by 32 · see citing works
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FOX, C. A. (1973) · Clinics in Endocrinology and Metabolism · doi:10.1016/s0300-595x(73)80013-1 · Cited by 11 · see citing works
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Roca, R. A., Garófalo, E. G., Martino, I., Píriz, H., Rieppi, G., Maraffi, M., Ohahian, C., & Gadola, L. (1978) · Biology of Reproduction · doi:10.1095/biolreprod19.3.552 · Cited by 10 · see citing works
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BackgroundMethodInfluentialGale, J. S., McIntosh, J. E. A., & McIntosh, R. P. (1988) · Biochemical Journal · doi:10.1042/bj2510251 · Cited by 9 · see citing works
Nachtmann, F., Krummen, K., Maxl, F., & Riemer, E. (1981) · Analytical profiles of drug substances · doi:10.1016/s0099-5428(08)60651-6 · Cited by 8 · see citing works
[23] The Talc-resin-trichloroacetic acid test for screening radioiodinated polypeptide hormones
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Ciabattoni, G. (1987) · Handbook of experimental pharmacology · doi:10.1007/978-3-642-71809-0_3 · Cited by 8 · see citing works
Studies concerning a radioimmunoassay for oxytocin
BASHORE, R. A. (1972) · American Journal of Obstetrics and Gynecology · doi:10.1016/s0002-9378(15)32499-6 · Cited by 7 · see citing works
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Czernichow, P., DRAY, F., & Ronne, J. (1976) · Immunochemistry · doi:10.1016/0019-2791(76)90373-6 · Cited by 6 · see citing works
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Wilson, N., & Greenhouse, V. Y. (1976) · Journal of Chromatography A · doi:10.1016/s0021-9673(00)81031-6 · Cited by 5 · see citing works
Diel, F., Schneider, E., & Quabbe, H.-J. (1977) · Clinical Chemistry and Laboratory Medicine (CCLM) · doi:10.1515/cclm.1977.15.1-12.669 · Cited by 5 · see citing works
Fránek, M., & Hruška, K. (1974) · Journal of Chromatography A · doi:10.1016/s0021-9673(01)85414-5 · Cited by 4 · see citing works
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Spector, S. (1982) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(82)84042-1 · Cited by 4 · see citing works
A voltage-clamp study of the adrenaline-hyperpolarization in the guinea-pig taenia coli.
Inomata, H., & Cy, K. (1972) · PubMed Central · Cited by 4 · see citing works
The radioimmunoassay of oxytocin in human plasma
Bossuyt‐Piron, C., Driessche, R. V., & Bossuyt, A. M. (1978) · International Journal of Nuclear Medicine and Biology · doi:10.1016/0047-0740(78)90049-9 · Cited by 3 · see citing works
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OGAWA, S., FUKUCHI, S., & MIURA, T. (1978) · Folia Endocrinologica Japonica · doi:10.1507/endocrine1927.54.2_107 · Cited by 3 · see citing works
Kagan, A., & Glick, S. M. (1978) · Elsevier eBooks · doi:10.1016/b978-0-12-379260-0.50022-4 · Cited by 2 · see citing works
Immunochemical recognition of oxytocin by antiserum to L-prolyl-leucyl-glycine amide
WATKINS, W. B., & Peek, J. C. (1985) · Neuroscience Letters · doi:10.1016/s0304-3940(85)80092-6 · Cited by 1 · see citing works
Nuklearmedizinische in vitro-Diagnostik
Finck, W. (1978) · Handbuch der medizinischen Radiologie · doi:10.1007/978-3-642-81170-8_8
(1978) · Laboratory techniques in biochemistry and molecular biology · doi:10.1016/s0075-7535(08)70184-0
- Authors
- CHARD, T., KITAU, M. J., & LANDON, J.
- Venue
- Journal of Endocrinology
- Publisher
- Bioscientifica
- Published
- 1970-02-01
- Volume
- 46
- Issue
- 2
- Pages
- 269-278
- ISSN
- 0022-0795 (ISSN-L)
- 1479-6805
- OpenAlex
- W2031531128
- Cited by
- 45
- References
- 0
- Citations by year
- 2016: 1
- FWCI
- 11.36
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Radiopharmaceutical Chemistry and Applications
- Keywords
- oxytocin, radioimmunoassay, iodine-131 labeling, radioiodination, antiserum production, lymph node injection, antibody separation, ammonium sulfate precipitation
- MeSH terms
- Adsorption, Quaternary Ammonium Compounds, Animals, Antibody Formation, Antigen-Antibody Complex, Carbon, Female, Humans, Injections, Intralymphatic, Iodine Isotopes, Lymph Nodes, Methods, Oxytocin, Chemical Precipitation, Rabbits, Radioimmunoassay, Sulfates
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