Production and Structural Characterization of Exopolysaccharides from Newly Isolated Probiotic Lactic Acid Bacteria
Abstract
In this work, four exopolysaccharide-producing lactic acid bacteria (LAB) strains, newly isolated from Tunisian spontaneously fermented foods and beverages, namely bovine and turkey meat sausages (BMS and TMS), date palm sap (DPS) and cow milk (CM), were identified as Leuconostoc citreum-BMS, Leuconostoc mesenteroides-TMS, Pediococcus pentosaceus-DPS and Leuconostoc pseudomesenteroides-CM, respectively. The isolated strains showed the ability to withstand simulated human gastrointestinal (GI) tract conditions (low pH, lysozyme, bile salts, pepsin and pancreatin) and showed high surface hydrophobicity (79-90%), besides their ability to act against Escherichia coli and Listeria monocytogenes and to produce exopolysaccharides (EPS). Therefore, these isolates can be served as potential probiotics. The produced EPS were growth-associated suggesting that they are primary metabolites. The molecular weights were higher than 10 6 Da using HPLC-SEC. 2D-NMR results indicated that all the samples were mixtures of dextran and levan, except for EPS-CM which was a levan-type EPS. Furthermore, the EPS samples showed an abitlity to inhibit and to disrupt pathogenic biofilms and showed high thermostability studied via differential scanning calorimetry (DSC) with melting points higher than 224°C making them promising to be used in thermal processed foods.
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High antibiofilm activity has been found for EPS synthetized by Leuconostoc citreum isolated from sausages.
“a high antibiofilm activity has been found for EPS synthetized by Leuconostoc citreum isolated from sausages [150].”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [150]
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- Authors
- Abid, Y., Casillo, A., Gharsallah, H., Joulak, I., Lanzetta, R., Corsaro, M. M., Attia, H., & Azabou, S.
- Affiliations
- University of Sfax (TN)
- University of Naples Federico II (IT)
- Corresponding author
- Azabou, Samia
- Venue
- International Journal of Biological Macromolecules
- Publisher
- Elsevier BV
- Published
- 2018-03
- Volume
- 108
- Pages
- 719-728
- ISSN
- 0141-8130 (ISSN-L)
- OpenAlex
- W2765250985
- Cited by
- 208
- References
- 55
- Citations by year
- 2026: 14 · 2025: 34 · 2024: 24 · 2023: 30 · 2022: 38 · 2021: 17 · 2020: 21 · 2019: 18 · 2018: 11 · 2017: 1
- FWCI
- 15.69
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- International Journal of Biological Macromolecules (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biopolymer Synthesis and Applications
- Keywords
- exopolysaccharides, lactic acid bacteria, probiotics, Leuconostoc citreum, Leuconostoc mesenteroides, Pediococcus pentosaceus, Leuconostoc pseudomesenteroides, dextran, levan, gastrointestinal survival, surface hydrophobicity, antibacterial activity, biofilm eradication, thermal stability, meat sausages, date palm sap, cow's milk
- MeSH terms
- Antibiosis, Anti-Bacterial Agents, Bile Acids and Salts, Humans, Molecular Weight, Monosaccharides, Magnetic Resonance Spectroscopy, Polysaccharides, Bacterial, Molecular Structure, Spectroscopy, Fourier Transform Infrared, Biofilms, Probiotics, Microbial Viability, Disk Diffusion Antimicrobial Tests, Lactobacillales
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