Dietary nitrate inhibits stress-induced gastric mucosal injury in the rat
Abstract
Dietary nitrate is reduced to nitrite by some oral bacteria and the resulting nitrite is converted to nitric oxide (NO) in acidic gastric juice. The aim of this study is to elucidate the pathophysiological role of dietary nitrate in the stomach. Intragastric administration of nitrate rapidly increased nitrate and NO in plasma and the gastric headspace, respectively. Water-immersion-restraint stress (WIRS) increased myeloperoxidase (MPO) activity in gastric mucosa and induced hemorrhagic erosions by a nitrate-inhibitable mechanism. In animals that had received either cardiac ligation or oral treatment with povidone-iodine, a potent bactericidal agent, administration of nitrate failed to increase gastric levels of NO and to inhibit WIRS-induced mucosal injury. WIRS decreased gastric mucosal blood flow by a mechanism which was inhibited by administration of nitrate. These data suggested that the enterosalivary cycle of nitrate and related metabolites consisted of gastrointestinal absorption and salivary secretion of nitrate, its conversion to nitrite by oral bacteria and then to NO in the stomach might play important roles in the protection of gastric mucosa from hazardous stress.
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Oral ingestion of nitrite into the acidic environment of the stomach produces NO that augments mucus secretion, antibacterial effects of gastric fluids, and ulcer healing.
“Moreover, oral ingestion of nitrite into the acidic environment of the stomach produces NO that augments mucus secretion, antibacterial effects of gastric fluids, and ulcer healing [93–97].”
From Inorganic nitrite therapy: historical perspective and future directions (2011) · cites this paper as [95]
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- Authors
- Miyoshi, M., Kasahara, E., Park, A.-M., Hiramoto, K., Minamiyama, Y., Takemura, S., Sato, E. F., & Inoue, M.
- Affiliations
- Osaka City University (JP)
- ORCID
- Venue
- Free Radical Research
- Publisher
- Informa UK Limited
- Published
- 2003-01
- Volume
- 37
- Issue
- 1
- Pages
- 85-90
- ISSN
- 1029-2470 (ISSN-L)
- 1071-5762
- OpenAlex
- W2149637168
- Cited by
- 74
- References
- 24
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 4 · 2023: 3 · 2022: 1 · 2020: 1 · 2019: 1 · 2017: 2 · 2016: 2 · 2015: 4 · 2014: 5 · 2013: 6 · 2012: 3
- FWCI
- 1.80
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Free Radical Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Keywords
- dietary nitrate, gastric mucosal injury, water immersion stress, nitric oxide, nitrite, oral bacteria, myeloperoxidase, gastric mucosal blood flow, stress-induced gastric damage, povidone-iodine
- MeSH terms
- Animals, Diet, Gastric Mucosa, Intestinal Absorption, Male, Neutrophils, Nitrates, Nitric Oxide, Nitrites, Regional Blood Flow, Saliva, Stress, Physiological, Rats, Wistar, Potassium Compounds, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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