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Age-associated analysis of oxidative stress parameters in human plasma and erythrocytes
Abstract
Oxidative damage accumulation in macromolecules has been considered as a cause of cellular damage and pathology. Rarely, the oxidative stress parameters in healthy humans related to the individual age have been reported. The purpose of this study was to examine the redox status in plasma and erythrocytes of healthy individuals and determine correlations between these parameters and the aging process. The following parameters were used: malondialdehyde (MDA), protein carbonyls (PCO), 4-hydroxy-2,3-trans-nonenal (HNE), reduced glutathione (GSH), glutathione disulfide (GSSG) and uric acid (UA) in blood and plasma samples of 194 healthy women and men of ages ranging from 18 to 84 years. The results indicate that the balance of oxidant and antioxidant systems in plasma shifts in favor of accelerated oxidation during ageing. That is demonstrated by increases of MDA, HNE, GSSG and by the slight decrease of erythrocytic GSH with age. As the content of UA is more determined by metabolic and nutritional influences than by the balance between prooxidants and antioxidants there was no significant age-related change observed. For plasma concentrations of HNE the first time age-dependent reference values for healthy humans are presented.
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“Studies reveal an age-related increase in the level of oxidatively modified proteins [10,11]”
From Protein oxidation - Formation mechanisms, detection and relevance as biomarkers in human diseases (2021) · cites this paper as [10]
In the citing paper
“Protein carbonyls have been found to be elevated in Alzheimer’s diseases, diabetes, renal diseases, and other disorders (Gil et al. 2006).”
From Reactive Oxygen Species, Toxicity, Oxidative Stress, and Antioxidants: Chronic Diseases and Aging (2023) · cites this paper as Gil et al. 2006
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Kędziora–Kornatowska, K., Szewczyk‐Golec, K., Czuczejko, J., de Lumen, K. V. M., Pawluk, H., Motyl, J., Karasek, M., & Kędziora, J. (2006) · Journal of Pineal Research · doi:10.1111/j.1600-079x.2006.00394.x · Cited by 46
Implication of oxidative stress as a cause of autoimmune hemolytic anemia in NZB mice
Iuchi, Y., Kibe, N., Tsunoda, S., Suzuki, S., Mikami, T., Okada, F., Uchida, K., & Fujii, J. (2010) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2010.01.012 · Cited by 46
Weber, D., Stuetz, W., Toussaint, O., Debacq-Chainiaux, F., Dollé, M. E. T., Jansen, E., Gonos, E. S., Franceschi, C., Sikora, E., Hervonen, A., et al. (2017) · Oxidative Medicine and Cellular Longevity · doi:10.1155/2017/1401452 · Cited by 46
Self-citationIuchi, Y., Okada, F., Takamiya, R., Kibe, N., Tsunoda, S., Nakajima, O., Toyoda, K., Nagae, R., Suematsu, M., Soga, T., et al. (2009) · Biochemical Journal · doi:10.1042/bj20090176 · Cited by 45
Age-dependent alteration of antioxidant defense system in hypertensive and type-2 diabetes patients
Okoduwa, S. I. R., Umar, I. A., Ibrahim, S., Bello, F., & Habila, N. (2015) · Journal of Diabetes & Metabolic Disorders · doi:10.1186/s40200-015-0164-z · Cited by 45
Winklhofer‐Roob, B. M., Faustmann, G., & Roob, J. M. (2017) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2017.04.345 · Cited by 44
Quantification of reduced glutathione by HPLC‐UV in erythrocytes of hemodialysis patients
García, S. C., Schott, K. L., Charão, M. F., Moro, A. M., Bulcão, R., Grotto, D., Valentini, J., Bohrer, D., Cardoso, S. G., & Pomblum, V. J. (2008) · Biomedical Chromatography · doi:10.1002/bmc.954 · Cited by 44
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RETRACTED: Proteomic studies on lactic acid bacteria: A review
Vinusha, K. S., Deepika, K., Johnson, T. S., Agrawal, G. K., & Rakwal, R. (2018) · Biochemistry and Biophysics Reports · doi:10.1016/j.bbrep.2018.04.009 · Cited by 43
RetractedSafety Assessment of the Human Milk-Isolated Probiotic Lactobacillus salivarius CECT5713
Lara‐Villoslada, F., Sierra, S., Díaz-Ropero, M. P., Olivares, M. M., & Xaus, J. (2007) · Journal of Dairy Science · doi:10.3168/jds.2006-685 · Cited by 42
Racine, M. L., & Dinenno, F. A. (2019) · The Journal of Physiology · doi:10.1113/jp278338 · Cited by 40
Optimization of selenizing conditions for Seleno-Lentinan and its characteristics
Ren, G., Li, K., Hu, Y., Yu, M., Qu, J., & Xu, X. (2015) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2015.08.012 · Cited by 39
Safety Assessment ofLactobacillus fermentumCECT5716, a probiotic strain isolated from human milk
Lara‐Villoslada, F., Sierra, S., Díaz-Ropero, M. P., Rodríguez, J. M., Xaus, J., & Olivares, M. M. (2009) · Journal of Dairy Research · doi:10.1017/s0022029909004014 · Cited by 39
Metadata
- Authors
- Gil, L., Siems, W., Mazurek, B., Gross, J., Schroeder, P., Voss, P., & Grune, T.
- Affiliations
- Instituto de Medicina Tropical “Pedro Kourí” (CU)
- Humboldt-Universität zu Berlin (DE)
- Heinrich Heine University Düsseldorf (DE)
- Corresponding author
- Voss, Peter
- Venue
- Free Radical Research
- Publisher
- Informa UK Limited
- Published
- 2006-01
- Volume
- 40
- Issue
- 5
- Pages
- 495-505
- ISSN
- 1029-2470 (ISSN-L)
- 1071-5762
- OpenAlex
- W2061523541
- Cited by
- 242
- References
- 59
- Citations by year
- 2026: 2 · 2025: 11 · 2024: 7 · 2023: 5 · 2022: 9 · 2021: 10 · 2020: 7 · 2019: 8 · 2018: 11 · 2017: 11 · 2016: 14 · 2015: 18 · 2014: 17 · 2013: 15 · 2012: 13
- FWCI
- 11.66
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Free Radical Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Alexander von Humboldt-Stiftung
- Deutsche Forschungsgemeinschaft
- Topics
- Health Sciences › Medicine › Biochemistry (medical) › Antioxidant Activity and Oxidative Stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Health Sciences › Medicine › Physiology (medical) › Exercise and Physiological Responses
- Keywords
- oxidative stress, aging, erythrocytes, plasma, malondialdehyde, protein carbonyls, reduced glutathione, glutathione disulfide, uric acid, age-related reference values, prooxidant-antioxidant balance, healthy adults
- MeSH terms
- Adolescent, Adult, Aged, Aged, 80 and over, Aging, Aldehydes, Erythrocytes, Female, Glutathione, Humans, Male, Malondialdehyde, Middle Aged, Oxidation-Reduction, Plasma, Sex Factors, Uric Acid, Biomarkers, Oxidative Stress, Glutathione Disulfide, Protein Carbonylation
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