Mechanical and Biological characterization of A Porous Poly‐L‐Lactic Acid‐Co‐ϵ‐Caprolactone scaffold for Tissue Engineering
Abstract
This article presents a method for making highly porous biodegradable scaffold that may ultimately be used for tissue engineering. Poly(L‐lactic‐co‐ϵ‐caprolactone) acid (70∶30) (PLCL) scaffold was produced using the solvent casting/leaching out method, which entails dissolving the polymer and adding a porogen that is then leached out by immersing the scaffold in distillated water. Tensile tests were performed for three types of scaffolds, namely pre‐wetted, dried, and UV‐irradiated scaffolds and their mechanical properties were measured. The pre‐wetted PLCL scaffold possessed a modulus of elasticity 0.92±0.09 MPa, a tensile strength of 0.12±0.03 MPa and an ultimate strain of 23±5.3%. No significant differences in the modulus elasticity, tensile strength, nor ultimate strain were found between the pre‐wetted, dried, and UV irradiated scaffolds. The PLCL scaffold was seeded by human fibroblasts in order to evaluate its biocompatibility by Alamar blue® assays. After 10 days of culture, the scaffolds showed good biocompatibility and allowed cell proliferation. However, the fibroblasts stayed essentially at the surface. This study shows the possibility to use the PLCL scaffold in dynamic mechanical conditions for tissue engineering
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PLCL tensile strength is closely related to the ratio of L-lactic acid to caprolactone, and higher L-lactide contents enable PLCL to exhibit higher tensile strength.
“In PLCL, the tensile strength is closely related to the ratio of L-lactic acid to caprolactone. The rigid structure of L-lactide monomers enables PLCL to exhibit higher tensile strength at higher L-lactide contents [52, 53].”
From Application of PLCL as a biodegradable polymer in biomedical engineering (2026) · cites this paper as [53]
PLCL tensile strength is typically between 20 and 60 MPa, depending on its molecular weight and proportion.
“Typically, the tensile strength is between 20 and 60 MPa, depending on its molecular weight and proportion.”
From Application of PLCL as a biodegradable polymer in biomedical engineering (2026) · cites this paper as [53]
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- Authors
- Vaquette, C., Frochot, C., Rahouadj, R., Muller, S., & Wang, X.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Vaquette, Cédryck
- Venue
- Soft Materials
- Publisher
- Informa UK Limited
- Published
- 2008-03-03
- Volume
- 6
- Issue
- 1
- Pages
- 25-33
- ISSN
- 1539-445X (ISSN-L)
- 1539-4468
- OpenAlex
- W2112784448
- Cited by
- 15
- References
- 15
- Citations by year
- 2026: 2 · 2021: 3 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 1 · 2014: 1 · 2012: 1
- FWCI
- 0.89
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Soft Materials (publishedVersion)
- Funders
- Centre National de la Recherche Scientifique
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Physical Sciences › Materials Science › Biomaterials › biodegradable polymer synthesis and properties
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Keywords
- porogen leaching, tensile properties, UV irradiation, human fibroblasts, cell seeding, biocompatibility, cell proliferation, tissue engineering scaffolds
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