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Ultrastructural relationship of the phagophore with surrounding organelles
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Additional identification problems may arise from damage caused by improper sample collection or fixation artefacts.
“Additional identification problems may arise from damage caused by improper sample collection or fixation artefacts [67,68,155,156].”
From Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1 (2021) · cites this paper as [68]
Autophagosomes, also referred to as initial autophagic vacuoles, typically have a double membrane.
“Autophagosomes, also referred to as initial autophagic vacuoles (AVi), typically have a double membrane.This structure is usually distinctly visible by EM as two parallel membrane layers (bilayers) separated by a relatively narrower or wider electrontranslucent cleft, even when applying the simplest routine EM fixation procedure (Figure 3A) [67,68].”
From Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1 (2021) · cites this paper as [68]
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LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing.
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Rowland, A. A., & Voeltz, G. K. (2012) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3440 · Cited by 1,018
Plasma membrane contributes to the formation of pre-autophagosomal structures
Ravikumar, B., Moreau, K., Jahreiss, L., Puri, C., & Rubinsztein, D. C. (2010) · Nature Cell Biology · doi:10.1038/ncb2078 · Cited by 902
An Atg9-containing compartment that functions in the early steps of autophagosome biogenesis
Mari, M., Griffith, J., Rieter, E., Krishnappa, L., Klionsky, D. J., & Reggiori, F. (2010) · Journal of Cell Biology · doi:10.1083/jcb.200912089 · Cited by 491
Seeing is believing: The impact of electron microscopy on autophagy research
Eskelinen, E.-L., Reggiori, F., Baba, M., Kovács, A. L., & Seglen, P. O. (2011) · Autophagy · doi:10.4161/auto.7.9.15760 · Cited by 281
Self-citationChapter 10 Monitoring Autophagy by Electron Microscopy in Mammalian Cells
Ylä‐Anttila, P., Vihinen, H., Jokitalo, E., & Eskelinen, E. (2009) · Methods in Enzymology · doi:10.1016/s0076-6879(08)03610-0 · Cited by 257
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Sorted by citations
Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).
Klionsky, D. J., Abdelmohsen, K., Abe, A., Abedin, M. J., Abeliovich, H., Acevedo Arozena, A., Adachi, H., Adams, C. M., Adams, P. D., Adeli, K., et al. (2016) · Autophagy · doi:10.1080/15548627.2015.1100356 · Cited by 5,894
Self-citationGuidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1
Klionsky, D. J., Abdel-Aziz, A. K., Abdelfatah, S., Abdellatif, M., Abdoli, A., Abel, S., Abeliovich, H., Abildgaard, M. H., Abudu, Y. P., Acevedo-Arozena, A., et al. (2021) · Autophagy · doi:10.1080/15548627.2020.1797280 · Cited by 2,897
Self-citationSubcellular connectomic analyses of energy networks in striated muscle
Bleck, C. K. E., Kim, Y., Willingham, T. B., & Glancy, B. (2018) · Nature Communications · doi:10.1038/s41467-018-07676-y · Cited by 150
Metadata
- Authors
- Biazik, J., Ylä-Anttila, P., Vihinen, H., Jokitalo, E., & Eskelinen, E.-L.
- Venue
- Autophagy
- Publisher
- Informa UK Limited
- Published
- 2015-02-25
- Volume
- 11
- Issue
- 3
- Pages
- 439-451
- ISSN
- 1554-8627
- 1554-8635
- Cited by
- 149
- References
- 9
- Language
- en
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