Modulation of pathogenic oral biofilms towards health with nisin probiotic
Open accessAbstract
Background Oral dysbiosis is an imbalance in the oral microbiome and is associated with a variety of oral and systemic diseases, including periodontal disease, caries, and head and neck/oral cancer. Although antibiotics can be used to control this dysbiosis, they can lead to adverse side effects and superinfections. Thus, novel strategies have been proposed to address these shortcomings. One strategy is the use of probiotics as antimicrobial agents, since they are considered safe for humans and the environment. Specifically, the Gram-positive Lactococcus lactis, a species present in the oral and gut microbiota, is able to produce nisin, which has been used worldwide for food preservation.Objective The objective of this study was to test whether a nisin probiotic can promote a healthier oral microbiome in pathogen-spiked oral biofilms.Results We found that L. lactis can prevent oral biofilm formation and disrupt 24-h and 48-h pre-formed biofilms. Finally, we demonstrate that both treatments, a nisin-producing L. lactis probiotic and nisin can decrease the levels of pathogens in the biofilms and return the diversity levels back to control or ‘healthy’ levels.Conclusion A nisin-producing probiotic, can be used to treat ‘disease-altered’ biofilms and promote healthier oral biofilms, which may be useful for improving patient oral health.
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In vitro biofilm culture systems using human saliva or defined media have served as a useful surrogate for oral biofilm research and have shed light on mechanisms involved in microbial adherence, species interactions, antibiofilm treatments, and organization of the microbial community.
“In vitro biofilm culture systems using human saliva or defined media have served as a useful surrogate for oral biofilm research and have shed light on the mechanisms involved in microbial adherence, species interactions, antibiofilm treatments, and organization of the microbial community.55, 56, 57, 58, 59”
From The oral microbiome: Role of key organisms and complex networks in oral health and disease (2021) · cites this paper as [57]
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The oral microbiome: Role of key organisms and complex networks in oral health and disease
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Self-citation
- Authors
- Radaic, A., Ye, C., Parks, B., Gao, L., Kuraji, R., Malone, E., Kamarajan, P., Zhan, L., & Kapila, Y. L.
- Affiliations
- University of California, San Francisco (US)
- Sun Yat-sen University (CN)
- Corresponding author
- Kapila, Yvonne L.
- ORCID
- Venue
- Journal of Oral Microbiology
- Publisher
- Informa UK Limited
- Published
- 2020-01
- Volume
- 12
- Issue
- 1
- Pages
- 1809302-1809302
- ISSN
- 2000-2297 (ISSN-L)
- PMC
- PMC7482728
- OpenAlex
- W3080159587
- Cited by
- 59
- References
- 49
- Citations by year
- 2026: 5 · 2025: 11 · 2024: 8 · 2023: 13 · 2022: 10 · 2021: 11 · 2020: 1
- FWCI
- 8.92
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Journal of Oral Microbiology (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- eScholarship (California Digital Library) (submittedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- eScholarship (California Digital Library) (submittedVersion)
- Funders
- AAP Sunstar (Innovation Award)
- NIH (R01 DE025225)
- Topics
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Keywords
- nisin, Lactococcus lactis, probiotics, dental plaque, oral dysbiosis, oral microbiota, biofilm eradication, periodontal disease, dental caries, oral cancer, antimicrobial peptides, oral health
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