CoChat
Sign Up
Cited by 214Antimicrobial Peptides and ActivitiesCancer Research and TreatmentsHead and Neck Cancer Studies

Nisin ZP, a Bacteriocin and Food Preservative, Inhibits Head and Neck Cancer Tumorigenesis and Prolongs Survival

Open access
Kamarajan, Pachiyappan, Hayami, Takayuki, Matte, Bibiana, Liu, Yang, Danciu, Theodora, Ramamoorthy, Ayyalusamy, Worden, Francis, Kapila, Sunil +1 more·2015·PLOS ONE
DOI 10.1371/journal.pone.0131008PMC PMC4489501
Open this paper in CoChat — free accountOpen PDF

Abstract

The use of small antimicrobial peptides or bacteriocins, like nisin, to treat cancer is a new approach that holds great promise. Nisin exemplifies this new approach because it has been used safely in humans for many years as a food preservative, and recent laboratory studies support its anti-tumor potential in head and neck cancer. Previously, we showed that nisin (2.5%, low content) has antitumor potential in head and neck squamous cell carcinoma (HNSCC) in vitro and in vivo. The current studies explored a naturally occurring variant of nisin (nisin ZP; 95%, high content) for its antitumor effects in vitro and in vivo. Nisin ZP induced the greatest level of apoptosis in HNSCC cells compared to low content nisin. HNSCC cells treated with increasing concentrations of nisin ZP exhibited increasing levels of apoptosis and decreasing levels of cell proliferation, clonogenic capacity, and sphere formation. Nisin ZP induced apoptosis through a calpain-dependent pathway in HNSCC cells but not in human oral keratinocytes. Nisin ZP also induced apoptosis dose-dependently in human umbilical vein endothelial cells (HUVEC) with concomitant decreases in vascular sprout formation in vitro and reduced intratumoral microvessel density in vivo. Nisin ZP reduced tumorigenesis in vivo and long-term treatment with nisin ZP extended survival. In addition, nisin treated mice exhibited normal organ histology with no evidence of inflammation, fibrosis or necrosis. In summary, nisin ZP exhibits greater antitumor effects than low content nisin, and thus has the potential to serve as a novel therapeutic for HNSCC.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1371/journal.pone.0131008

Cites

62 of 62 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Lactococcus lactis produces nisin, a bacteriocin that has been shown to reduce oral tumorigenesis and extend the life span of mice with tumors.

    “Lactococcus lactis, a commensal of the oral microbiota, produces nisin, a bacteriocin that has been shown to reduce oral tumorigenesis and extend the life span of mice with tumors.165, 166”

    From The oral microbiome: Role of key organisms and complex networks in oral health and disease (2021) · cites this paper as [166]

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

1 of the 63 references couldn’t be matched to a known paper.

Cited by

Sorted by citations

Related

Authors
Kamarajan, P., Hayami, T., Matte, B., Liu, Y., Danciu, T., Ramamoorthy, A., Worden, F., Kapila, S., & Kapila, Y.
Affiliations
University of Michigan (US)
Corresponding author
Kapila, Yvonne
ORCID
Venue
PLOS ONE
Publisher
Public Library of Science (PLoS)
Published
2015-07-01
Volume
10
Issue
7
Pages
e0131008
ISSN
1932-6203 (ISSN-L)
DOI
10.1371/journal.pone.0131008
PMC
PMC4489501
OpenAlex
W1871229721
Cited by
214
References
63
Citations by year
2026: 12 · 2025: 22 · 2024: 26 · 2023: 27 · 2022: 31 · 2021: 33 · 2020: 24 · 2019: 9 · 2018: 9 · 2017: 13 · 2016: 5 · 2015: 3
FWCI
8.42
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • eScholarship (California Digital Library) (submittedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • University of Michigan
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico
  • Peking University
  • Ciência sem Fronteiras
  • Division of Graduate Education
Topics
  • Life Sciences › Immunology and Microbiology › Microbiology › Antimicrobial Peptides and Activities
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Cancer Research and Treatments
  • Health Sciences › Medicine › Otorhinolaryngology › Head and Neck Cancer Studies
Keywords
bacteriocins, antimicrobial peptides, head and neck squamous cell carcinoma, anticancer activity, apoptosis, calpain, tumor angiogenesis, microvessel density, carcinogenesis, survival, food preservatives, oral keratinocytes, endothelial cells
MeSH terms
Animals, Carcinoma, Squamous Cell, Dose-Response Relationship, Drug, Flow Cytometry, Head and Neck Neoplasms, Humans, Mice, Nude, Mouth Neoplasms, Neoplasms, Experimental, Neovascularization, Pathologic, Nisin, Blotting, Western, Apoptosis, Cell Line, Tumor, Cell Proliferation, Mice, Microvessels, Carcinogenesis

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review