The DOI matches its Crossref record; 54 of the 54 references it cites were found in a registry or an index.
Comparison of small extracellular vesicles isolated from plasma by ultracentrifugation or size‐exclusion chromatography: yield, purity and functional potential
Open accessAbstract
Interest in small extracellular vesicles (sEVs) as functional carriers of proteins and nucleic acids is growing continuously. There are large numbers of sEVs in the blood, but lack of standardised methods for sEV isolation greatly limits our ability to study them. In this report, we use rat plasma to systematically compare two commonly used techniques for isolation of sEVs: ultracentrifugation (UC‐sEVs) and size‐exclusion chromatography (SEC‐sEVs). SEC‐sEVs had higher particle number, protein content, particle/protein ratios and sEV marker signal than UC‐sEVs. However, SEC‐sEVs also contained greater amounts of APOB+ lipoproteins and large quantities of non‐sEV protein. sEV marker signal correlated very well with both particle number and protein content in UC‐sEVs but not in all of the SEC‐sEV fractions. Functionally, both UC‐sEVs and SEC‐sEVs isolates contained a variety of proangiogenic factors (with endothelin‐1 being the most abundant) and stimulated migration of endothelial cells. However, there was no evident correlation between the promigratory potential and the quantity of sEVs added, indicating that non‐vesicular co‐isolates may contribute to the promigratory effects. Overall, our findings suggest that UC provides plasma sEVs of lower yields, but markedly higher purity compared to SEC. Furthermore, we show that the functional activity of sEVs can depend on the isolation method used and does not solely reflect the sEV quantity. These findings are of importance when working with sEVs isolated from plasma‐ or serum‐containing conditioned medium.
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Cites
Shedding light on the cell biology of extracellular vesicles
van Niel, G., D'Angelo, G., & Raposo, G. (2018) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2017.125 · Cited by 8,369
Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids
Théry, C., Amigorena, S., Raposo, G., & Clayton, A. (2006) · Current Protocols in Cell Biology · doi:10.1002/0471143030.cb0322s30 · Cited by 5,766
Kowal, J., Arras, G., Colombo, M., Jouve, M., Morath, J. P., Primdal-Bengtson, B., Dingli, F., Loew, D., Tkach, M., & Théry, C. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1521230113 · Cited by 3,426
Witwer, K. W., Buzás, E. I., Bemis, L. T., Bora, A., Lässer, C., Lötvall, J., Nolte‐‘t Hoen, E. N., Piper, M. G., Sivaraman, S., Skog, J., et al. (2013) · Journal of Extracellular Vesicles · doi:10.3402/jev.v2i0.20360 · Cited by 2,421
Pan, B.-T., & Johnstone, R. M. (1983) · Cell · doi:10.1016/0092-8674(83)90040-5 · Cited by 2,032
Harding, C., Heuser, J., & Stahl, P. (1983) · The Journal of Cell Biology · doi:10.1083/jcb.97.2.329 · Cited by 1,775
Optimized exosome isolation protocol for cell culture supernatant and human plasma
Lobb, R. J., Becker, M., Wen Wen, S., Wong, C. S. F., Wiegmans, A. P., Leimgruber, A., & Möller, A. (2015) · Journal of Extracellular Vesicles · doi:10.3402/jev.v4.27031 · Cited by 1,705
Zhang, H., Freitas, D., Kim, H. S., Fabijanic, K., Li, Z., Chen, H., Mark, M. T., Molina, H., Martin, A. B., Bojmar, L., et al. (2018) · Nature Cell Biology · doi:10.1038/s41556-018-0040-4 · Cited by 1,613
Double-stranded DNA in exosomes: a novel biomarker in cancer detection
Thakur, B. K., Zhang, H., Becker, A., Matei, I., Huang, Y., Costa-Silva, B., Zheng, Y., Hoshino, A., Brazier, H., Xiang, J., et al. (2014) · Cell Research · doi:10.1038/cr.2014.44 · Cited by 1,596
EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research
EV-TRACK Consortium, Van Deun, J., Mestdagh, P., Agostinis, P., Akay, Ö., Anand, S., Anckaert, J., Martinez, Z. A., Baetens, T., Beghein, E., et al. (2017) · Nature Methods · doi:10.1038/nmeth.4185 · Cited by 1,284
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Application of exosomes as liquid biopsy in clinical diagnosis
Zhou, B., Xu, K., Zheng, X., Chen, T., Wang, J., Song, Y., Shao, Y., & Zheng, S. (2020) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-020-00258-9 · Cited by 685
Tian, Y., Gong, M., Hu, Y., Liu, H., Zhang, W., Zhang, M., Hu, X., Aubert, D., Zhu, S., Wu, L., et al. (2020) · Journal of Extracellular Vesicles · doi:10.1080/20013078.2019.1697028 · Cited by 643
Lozano‐Andrés, E., Enciso‐Martinez, A., Gijsbers, A., Ridolfi, A., Van Niel, G., Libregts, S. F. W. M., Pinheiro, C., van Herwijnen, M. J. C., Hendrix, A., Brucale, M., et al. (2023) · Journal of Extracellular Vesicles · doi:10.1002/jev2.12376 · Cited by 56
Extracellular vesicles and lipoproteins – Smart messengers of blood cells in the circulation
Nguyen, P. H. D., Le, A. H., Pek, J. S. Q., Pham, T. T., Jayasinghe, M. K., Do, D. V., Phung, C. D., & Le, M. T. N. (2022) · Journal of Extracellular Biology · doi:10.1002/jex2.49 · Cited by 31
Extracellular Vesicles and Their Interplay with Biological Membranes
Yeo Xian Ping, J., Raj Neupane, Y., & Pastorin, G. (2022) · Physiology · doi:10.5772/intechopen.101297 · Cited by 9
Metadata
- Authors
- Takov, K., Yellon, D. M., & Davidson, S. M.
- Venue
- Journal of Extracellular Vesicles
- Publisher
- Wiley
- Published
- 2018-12-28
- Volume
- 8
- Issue
- 1
- Pages
- 1560809-1560809
- ISSN
- 2001-3078 (ISSN-L)
- OpenAlex
- W2908122377
- Cited by
- 417
- References
- 54
- FWCI
- 11.69
- Type
- journal article
- Language
- en
- Open access
- gold
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- Takov, K., Yellon, D. M., & Davidson, S. M. (2019). Comparison of small extracellular vesicles isolated from plasma by ultracentrifugation or size‐exclusion chromatography: yield, purity and functional potential. Journal of Extracellular Vesicles. https://doi.org/10.1080/20013078.2018.1560809
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