Lactobacillus plantarum ‐derived extracellular vesicles induce anti‐inflammatory M2 macrophage polarization in vitro
Open accessAbstract
Probiotics offer various health benefits. Lactobacillus plantarum has been used for decades to enhance human intestinal mucosal immunity and improve skin barrier integrity. Extracellular vesicles (EVs) derived from eukaryotic or prokaryotic cells have been recognized as efficient carriers for delivery of biomolecules to recipient cells, and to efficiently regulate human pathophysiology. However, the mechanism underlying the beneficial effects of probiotic bacteria‐derived EVs on human skin is unclear. Herein, we investigated how L. plantarum ‐derived EVs (LEVs) exert beneficial effects on human skin by examining the effect of LEVs on cutaneous immunity, particularly on macrophage polarization. LEVs promoted differentiation of human monocytic THP1 cells towards an anti‐inflammatory M2 phenotype, especially M2b, by inducing biased expression of cell‐surface markers and cytokines associated with M2 macrophages. Pre‐ or post‐treatment with LEVs under inflammatory M1 macrophage‐favouring conditions, induced by LPS and interferon‐γ, inhibited M1‐associated surface marker, HLA‐DRα expression. Moreover, LEV treatment significantly induced expression of macrophage‐characteristic cytokines, IL‐1β, GM‐CSF and the representative anti‐inflammatory cytokine, IL‐10, in human skin organ cultures. Hence, LEVs can trigger M2 macrophage polarization in vitro , and induce an anti‐inflammatory phenomenon in the human skin, and may be a potent anti‐inflammatory strategy to alleviate hyperinflammatory skin conditions.
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Applications of Plant-Derived Extracellular Vesicles in Medicine
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- Authors
- Kim, W., Lee, E. J., Bae, I., Myoung, K., Kim, S. T., Park, P. J., Lee, K., Pham, A. V. Q., Ko, J., Oh, S. H., & Cho, E.
- Affiliations
- Daegu Haany University (KR)
- Yonsei University (KR)
- Amorepacific (South Korea) (KR)
- Basic Research and Innovation Division R&D Center, Amorepacific Corporation Yongin Republic of Korea
- Korea University (KR)
- Corresponding author
- Cho, Eun‐Gyung
- ORCID
- Venue
- Journal of Extracellular Vesicles
- Publisher
- Wiley
- Published
- 2020-07-17
- Volume
- 9
- Issue
- 1
- Pages
- 1793514-1793514
- ISSN
- 2001-3078 (ISSN-L)
- PMC
- PMC7480564
- OpenAlex
- W3042385885
- Cited by
- 232
- References
- 9
- Citations by year
- 2026: 58 · 2025: 66 · 2024: 42 · 2023: 25 · 2022: 26 · 2021: 13 · 2020: 2
- FWCI
- 5.35
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Journal of Extracellular Vesicles (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Ministry of Science and ICT, Republic of Korea under the National Research Foundation (NRF-2017R1D1A1B03033241, NRF-2018R1D1A1B07043290, NRF-2018R1A5A2025272)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Immunology and Microbiology › Immunology › Immune cells in cancer
- Keywords
- Lactobacillus plantarum, extracellular vesicles, M2 macrophage polarization, M2b macrophages, anti-inflammatory activity, skin immunity, THP-1 cells, IL-10, GM-CSF, IL-1β, interferon-gamma, lipopolysaccharide, probiotics, skin barrier
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