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Cited by 130Neuroscience and Neuropharmacology ResearchNeurogenesis and neuroplasticity mechanismsNeuroinflammation and Neurodegeneration Mechanisms

Three-dimensional morphology of astrocytes and oligodendrocytes in the intact mouse optic nerve

Butt, A. M., Colquhoun, K., Tutton, M., Berry, M.·1994·Journal of Neurocytology
DOI 10.1007/bf01184071
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Abstract

The three-dimensional morphology of astrocytes and oligodendrocytes was analysed in the isolated intact mature mouse optic nerve, by correlating laser scanning confocal microscopy and camera lucida drawings of single cells, dye-filled with lysinated rhodamine dextran or horseradish peroxidase, respectively. These techniques enabled the entire process field of single dye-filled cells to be visualized in all planes and resolved the fine details of glial morphology. Morphometric analysis showed that the processes of all astrocytes had branches ending at the pial surface, on blood vessels, and freely in the nerve; branches ending in the nerve were described to end at nodes of Ranvier in the accompanying paper. Astrocytes were classified into a single morphological population in which each cell subserved multiple functions. The results of this study do not support the contention that astrocytes can be subdivided into two morphological and functional subtypes, namely type-1 and type-2, which have process ending either at the glia limitans or at nodes, respectively. Three-dimensional analysis of oligodendrocyte units, defined as the oligodendrocyte, its processes and the axons it ensheaths, showed the provision of single myelin segments for an average of 19 nearby axons (range 12-35) with a mean internodal length of 138 microns (range 50-350 microns). Mouse optic nerve oligodendrocytes were a homogeneous population and were markedly similar to those in the rat optic nerve. The results of our analysis of oligodendrocyte morphology are consistent with the view that the number and internodal length of myelin sheaths supported by a single oligodendrocyte are related to the diameter of the ensheathed axons.

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  • Internode length and the number of internodes elaborated per OL vary greatly between CNS regions.

    “Internode length and the number of internodes elaborated per OL vary greatly between CNS regions (Butt et al., 1994; Murtie et al., 2007; Chong et al., 2012)”

    From Oligodendrocyte Dynamics in the Healthy Adult CNS: Evidence for Myelin Remodeling (2013) · cites this paper as Butt et al., 1994

  • Butt et al. (1994) measured randomly selected, dye-injected OLs in optic nerves from mice aged between P30 and P60, and counted ∼19 internodes per cell with an average length ∼138 μm.

    “Butt et al. (1994) measured randomly selected, dye-injected OLs in optic nerves from mice aged between P30 and P60, and counted ∼19 internodes per cell with an average length ∼138 μm.”

    From Oligodendrocyte Dynamics in the Healthy Adult CNS: Evidence for Myelin Remodeling (2013) · cites this paper as Butt et al. (1994)

  • There is a progressive decrease in the lengths of newly formed internodes with advancing OL birthdate, from ∼140 μm in the early postnatal period (∼P10-P20), to ∼80 μm after P30, to ∼40 μm after P50, to ∼20 μm after P120.

    “Our data and those of Butt et al. (1994) show that there is a progressive decrease in the lengths of newly formed internodes with advancing OL birthdate, from ∼140 μm in the early postnatal period (∼P10–P20), to ∼80 μm after P30, to ∼40 μm after P50, to ∼20 μm after P120 (Figure 6F).”

    From Oligodendrocyte Dynamics in the Healthy Adult CNS: Evidence for Myelin Remodeling (2013) · cites this paper as Butt et al. (1994)

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Authors
Butt, A. M., Colquhoun, K., Tutton, M., & Berry, M.
Affiliations
Guy's Hospital (GB)
ORCID
Venue
Journal of Neurocytology
Publisher
Springer Science and Business Media LLC
Published
1994-08
Volume
23
Issue
8
Pages
469-485
ISSN
  • 0300-4864 (ISSN-L)
  • 1573-7381
DOI
10.1007/bf01184071
OpenAlex
W2079377588
Cited by
130
References
94
Citations by year
2026: 2 · 2025: 3 · 2024: 5 · 2023: 4 · 2022: 8 · 2021: 5 · 2020: 6 · 2019: 4 · 2018: 5 · 2017: 4 · 2016: 3 · 2015: 2 · 2014: 7 · 2013: 9 · 2012: 4
FWCI
1.81
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • Journal of Neurocytology (publishedVersion)
  • PubMed (publishedVersion)
Funders
Wellcome Trust
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
Keywords
astrocytes, oligodendrocytes, optic nerve, three-dimensional morphology, glial morphology, astrocyte subtypes, myelination, internode length, axon diameter, glia limitans, nodes of Ranvier
MeSH terms
Animals, Astrocytes, Dextrans, Fluorescent Dyes, Horseradish Peroxidase, Mice, Inbred BALB C, Oligodendroglia, Optic Nerve, Reference Values, Rhodamines, Microscopy, Confocal, Mice, In Vitro Techniques

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