Cited by 873Microtubule and mitosis dynamicsGenetic and Kidney Cyst DiseasesCancer Treatment and Pharmacology

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Microtubules containing acetylated alpha-tubulin in mammalian cells in culture.

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Piperno, G, LeDizet, M, Chang, X J·1987·The Journal of cell biology

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Abstract

The subcellular distribution of microtubules containing acetylated alpha-tubulin in mammalian cells in culture was analyzed with 6-11B-1, a monoclonal antibody specific for acetylated alpha-tubulin. Cultures of 3T3, HeLa, and PtK2 cells were grown on coverslips and observed by immunofluorescence microscopy after double-staining by 6-11B-1 and B-5-1-2, a monoclonal antibody specific for all alpha-tubulins. The antibody 6-11B-1 binds to primary cilia, centrioles, mitotic spindles, midbodies, and to subsets of cytoplasmic microtubules in 3T3 and HeLa cells, but not in PtK2 cells. These observations confirm that the acetylation of alpha-tubulin is a modification occurring in different microtubule structures and in a variety of eukaryotic cells. Some features of the acetylation of cytoplasmic microtubules of mammalian cells are also described here. First, acetylated alpha-tubulin is present in microtubules that, under depolymerizing conditions, are more stable than the majority of cytoplasmic microtubules. In addition to the specific microtubule frameworks already mentioned, cytoplasmic microtubules resistant to nocodazole or colchicine, but not cold-resistant microtubules, contain more acetylated alpha-tubulin than the rest of cellular microtubules. Second, the alpha-tubulin in all cytoplasmic microtubules of 3T3 and HeLa cells becomes acetylated in the presence of taxol, a drug that stabilizes microtubules. Third, acetylation and deacetylation of cytoplasmic microtubules are reversible in cells released from exposure to 0 degrees C or antimitotic drugs. Fourth, the epitope recognized by the antibody 6-11B-1 is not absolutely necessary for cell growth and division. This epitope is absent in PtK2 cells. The acetylation of alpha-tubulin could regulate the presence of microtubules in specific intracellular spaces by selective stabilization.

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Taxol stabilizes microtubules in mouse fibroblast cells.
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Self-citation
Taxol binds to polymerized tubulin in vitro
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Self-citation
Reversal of the posttranslational modification on Chlamydomonas flagellar alpha-tubulin occurs during flagellar resorption.
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Authors: Piperno, G., LeDizet, M., & Chang, X. J.
Affiliations: Rockefeller University (US)
ORCID: LeDizet, M
Venue: The Journal of cell biology
Publisher: Rockefeller University Press
Published: 1987-02-01
Volume: 104
Issue: 2
Pages: 289-302
ISSN: 0021-9525 (ISSN-L)
1540-8140
DOI: 10.1083/jcb.104.2.289
PMC: PMC2114420
OpenAlex: W2155768517
Cited by: 873
References: 13
Citations by year: 2026: 15 · 2025: 18 · 2024: 23 · 2023: 27 · 2022: 19 · 2021: 30 · 2020: 24 · 2019: 29 · 2018: 32 · 2017: 28 · 2016: 28 · 2015: 34 · 2014: 33 · 2013: 34 · 2012: 36
FWCI: 15.45
Type: journal article
Language: en
Open access: bronze
doi.org
Locations: The Journal of Cell Biology (publishedVersion)
PubMed Central (publishedVersion, cc-by-nc-sa)
PubMed (publishedVersion)
Funders: National Institute of General Medical Sciences (GM28702)
National Center for Research Resources (S07 RR077065)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Kidney Cyst Diseases
Health Sciences › Medicine › Oncology › Cancer Treatment and Pharmacology
Keywords: acetylated alpha-tubulin, alpha-tubulin acetylation, microtubules, cytoplasmic microtubules, primary cilia, centrioles, mitotic spindle, midbody, microtubule stabilization, paclitaxel, nocodazole, colchicine, deacetylation, 3T3 fibroblasts, HeLa cells, PtK2 cells
MeSH terms: Acetylation, Alkaloids, Animals, Cell Line, Cells, Cultured, Cold Temperature, Fluorescent Antibody Technique, HeLa Cells, Humans, Kinetics, Microtubules, Tubulin, Paclitaxel, Mice

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Microtubules containing acetylated alpha-tubulin in mammalian cells in culture.

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