The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.
Evidence for the presence of a proton pump of the vacuolar H(+)-ATPase type in the ruffled borders of osteoclasts.
Open accessAbstract
Microsomal membrane vesicles prepared either from chicken medullary bone or isolated osteoclasts were shown to have ATP-dependent H(+)-transport activity. This activity was N-ethylmaleimide-sensitive but resistant to oligomycin and orthovanadate, suggesting a vacuolar-type ATPase. Furthermore, immunological cross-reactivity of 60- and 70-kD osteoclast membrane antigens with Neurospora crassa vacuolar ATPase was observed when analyzed by immunoblotting. Same antibodies labeled only osteoclasts in chicken and rat bone in immunohistochemistry. Immunoelectronmicroscopy localized these antigens in apical membranes of rat osteoclasts and kidney intercalated cells of inner stripe of outer medulla. Pretreatment of animals with parathyroid hormone enhanced the immunoreaction in the apical membranes of osteoclasts. No immunoreaction was seen in osteoclasts when antibodies against gastric H+,K(+)-ATPase were used. These results suggest that osteoclast resorbs bone by secreting protons through vacuolar H(+)-ATPase.
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Cites
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Cited by
Sato, M., Grasser, W., Endo, N., Akins, R., Simmons, H., Thompson, D. D., Golub, E., & Rodan, G. A. (1991) · Journal of Clinical Investigation · doi:10.1172/jci115539 · Cited by 1,004
Effect of Proton Pump Inhibitor Pretreatment on Resistance of Solid Tumors to Cytotoxic Drugs
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Molecular and cellular biology of alveolar bone
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Tumor Acidity, Chemoresistance and Proton Pump Inhibitors
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Mechanism of osteoclast mediated bone resorption—rationale for the design of new therapeutics
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Metadata
- Authors
- Väänänen, H. K., Karhukorpi, E. K., Sundquist, K., Wallmark, B., Roininen, I., Hentunen, T., Tuukkanen, J., & Lakkakorpi, P.
- Venue
- The Journal of cell biology
- Publisher
- Rockefeller University Press
- Published
- 1990-09-01
- Volume
- 111
- Issue
- 3
- Pages
- 1305-1311
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2106091071
- Cited by
- 391
- References
- 23
- FWCI
- 5.94
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Evidence for the presence of a proton pump of the vacuolar H(+)-ATPase type in the ruffled borders of osteoclasts. https://app.cochat.ai/papers/10.1083/jcb.111.3.1305
- The paper
- Väänänen, H. K., Karhukorpi, E. K., Sundquist, K., Wallmark, B., Roininen, I., Hentunen, T., Tuukkanen, J., & Lakkakorpi, P. (1990). Evidence for the presence of a proton pump of the vacuolar H(+)-ATPase type in the ruffled borders of osteoclasts. The Journal of cell biology. https://doi.org/10.1083/jcb.111.3.1305
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
